30,535 results

1. p405MET25

   Plasmid

   #15961

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   MET25 promoter is between SacI and XbaI restricti…

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-TEV-FUS

   Plasmid

   #29629

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE60-EFTu-His

   Plasmid

   #124127

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Elongation factor Tu (tufB Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaG94-15

   Plasmid

   #228971

   Purpose

   For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-15
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaMIgG-14

   Plasmid

   #228994

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-14
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-LaTdT-39

   Plasmid

   #228980

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-39
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSH624-ssr

   Plasmid

   #199241

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   single-stranded DNA recombineering
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq-Ptrc

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCP20T-ecfp

   Plasmid

   #78463

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-2TK cyclin T1 (P#468)

   Plasmid

   #14607

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

   Insert

   cyclin T1 (CCNT1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pUA66 theta GFP

    Plasmid

    #105607

    Purpose

    Expresses GFP with no promoter

    Depositor

    Pamela Silver

    Article

    Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    No Promoter

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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