30,262 results
-
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pASTA3
Plasmid#24657DepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT
Plasmid#46987PurposeInducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
UseTagsGST and PreScission cleavage siteExpressionBacterialMutationcontains amino acids 190–530 of accession NP_0562…PromotertacAvailable sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG 3.1
Plasmid#193802PurposeProtein production of ChemoG3.1 in bacteriaDepositorInsertChemoG 3.1
UseTags10xHisExpressionBacterialMutationEGFP: A206K, T225RPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-24
Plasmid#79939PurposePH-Late1-I3-01-mycDepositorInsertEPN-24
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_ABCE1-Halo
Plasmid#175335PurposeProduction of HaloTag fusion proteinDepositorInsertABCE1 (ABCE1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176854PurposeExpresses membrane bound biotin ligase, BioID2, in neuronsDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)
Plasmid#169161PurposeTo express nsp10-nsp14 fusion protein in E. coliDepositorInsert14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
UseTags14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSP3535VA
Plasmid#46887PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…TagsExpressionBacterialMutationPromoterPnisAAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET Flag TEV LIC cloning vector (1L)
Plasmid#29662DepositorTypeEmpty backboneUseTagsFlag and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-Galectin-8 FL
Plasmid#68805PurposeExpress Galectin-8 FL protein in Bacterial cellsDepositorInsertGalectin-8 (LGALS8 Human)
UseTagsN-terminal 6-histidine-tagExpressionBacterialMutationNo mutationPromoterT7Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPGC-K
Plasmid#174581PurposepOPIN-GG expression vector compatible with C-terminal tagging, Kanamycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT137-splitD
Plasmid#122606Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastMutationPromoternoAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorInsertLasI
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-pro-IL18
Plasmid#183389PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertpro-IL18 (Il18 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28aAviHisCD72cCTLD
Plasmid#129066Purposegeneration of biotinylated mouse CD72c CTLD protein using bacteriaDepositorInsertCD72c (Cd72 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW334e
Plasmid#237315Purposeexpresses A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2DepositorInsertA. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-ktrA-yfp
Plasmid#227640Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertktrA-yfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only