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Plasmid#220229PurposeBacterial expression of AcK in E. coli BL21_DE3 (pLys) or similarDepositorInsertAcetate kinase (ackA )
UseTagsHistagExpressionBacterialMutationPromoterT7Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC31
Plasmid#209996PurposeContains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmidsDepositorInsert3' Connector (3C2SN)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC32
Plasmid#209997PurposeContains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmidsDepositorInsert3' Connector (3C5CSN)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC52
Plasmid#209991PurposeContains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C2SN)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC51
Plasmid#209990PurposeContains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C1CSN)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LASP1_LIM
Plasmid#109780PurposeProtein expression and purification of LASP1_LIMDepositorInsertLASP1_LIM (LASP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbE7k-RFP
Plasmid#35315DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Fast-FT
Plasmid#31916DepositorInsertFast-FT
UseTagsHisExpressionBacterialMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PP7v3
Plasmid#107230PurposeExpresses 24x optimized PP7 stem loops (version 3) in bacterial cellsDepositorInsert24xPP7v3
UseTags3x FLAG-tag and P2A siteExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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6xHis-MBP-BrCas12b
Plasmid#182276PurposeExpresses recombinant BrCas12bDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis, T7, Thrombin siteExpressionBacterialMutationPromoterT7Available sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbA2c-RFP
Plasmid#35326DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTetAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR2F2_Hormone-recep
Plasmid#109826PurposeProtein expression and purification of NR2F2_Hormone-recepDepositorInsertNR2F2_Hormone-recep (NR2F2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-hTFIIB
Plasmid#171084PurposeExpresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIBDepositorInsertTF2B (GTF2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR-SF61
Plasmid#163916PurposetRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codonDepositorInsertSF5Phe tRNA synthetase
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-8
Plasmid#172772PurposeBacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135T
Plasmid#160433PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to ThrDepositorInsertE. coli AlkB
UseTagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Threonine; deleted t…PromoterT7Available sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mNectarine
Plasmid#21717PurposeBacterial expression of pH-sensitive monomeric mNectarine red fluorescent proteinDepositorInsertmNectarine fluorescent proteins
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSP64T (DM#111)
Plasmid#15030DepositorInsertbeta-globin 5' and 3' UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1
Plasmid#81047Purposemammalian expression of Zdk1DepositorInsertZdk1
UseTags6x His tag, FLAG, and mVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only