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32,167 results

Showing: 7861 - 7890 of 32167 results

  2. pTriEx-mPA-GFP-PA-Rac1

    Plasmid
    #22022
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Tags
    6X His and mPA-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  5. pUC19-link-sfGFP-FLAG:Kan

    Plasmid
    #211833
    Purpose
    Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.
    Depositor
    Peng Chen
    Insert
    sfGFP-FLAG-Kan
    Expression
    Bacterial
    Mutation
    NA
    Available Since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211833-plasmid-map-sequence-id-418068

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  6. pOPS0254

    Plasmid
    #115506
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115506-plasmid-map-sequence-id-224294

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  10. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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  12. 4RCI

    Plasmid
    #64653
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. CD Ugi1

    Plasmid
    #177194
    Purpose
    Greengate entry vector pGGC000 modified to contain bladderwort insulator 1
    Depositor
    Wayne Parrott
    Article
    Park et al bioRxiv 2021.11.04.467280
    Insert
    Utricularia gibba insulator 1
    Expression
    Bacterial
    Available Since
    June 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177194-plasmid-map-sequence-id-361543

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  16. p006-GFP-Logic-AND

    Plasmid
    #112237
    Purpose
    GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Inserts
    GFP under control of doubly-repressible promoter
    GFP
    araC
    Expression
    Bacterial
    Promoter
    AND-gate and pLacI
    Available Since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112237-plasmid-map-sequence-id-218819

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  18. YWHAB

    Plasmid
    #39128
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YWHAB (YWHAB Human)
    Tags
    Tev His6
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. 5T5G (SETD8)

    Plasmid
    #101886
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD8 (KMT5A Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101886-plasmid-map-sequence-id-194755

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  21. BRDT

    Plasmid
    #38898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRDT (BRDT Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available Since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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Showing: 7861 - 7890 of 32167 results