31,059 results
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Plasmid#36920DepositorInsertPaBphP-PCD
TagsHisExpressionBacterialMutationContains the photosensory core domain coding regi…PromoterT7Available SinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp8
Plasmid#156477PurposeBacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tagDepositorInsertNsp8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDI
Plasmid#60555Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneTagsHis6-hPDIExpressionBacterialAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPA016 (delta 86-130 Parkin)
Plasmid#102357Purposeexpresses Rat Parkin with residues 86-130 deletedDepositorInsertParkin (Prkn Rat)
TagsGSTExpressionBacterialMutationdeleted residues 86 to 130PromotertacAvailable SinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_li_Tq2CFP
Plasmid#136971PurposeGoldengate/Greengate C-tag module, containing Tq2CFP fused to a flexible linkerDepositorInsertTq2CFP
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
RBKS
Plasmid#25544PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPDS1-mEGFP-IRES-puro2b
Plasmid#26464DepositorAvailable SinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
GST-FUS 465-526
Plasmid#44983DepositorInsertFUS (465-526aa) (FUS Human)
TagsGSTExpressionBacterialMutationcontains amino acids 465-526PromotertacAvailable SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo1
Plasmid#157805PurposeExpression of synthetic protein composed of eGFP and BRD4 bromodomain #1DepositorInserteGFP-bromo1
TagsHis6 and MBPExpressionBacterialPromoterpTACAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-TPR
Plasmid#190822PurposeFor expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags6x HisExpressionBacterialMutationC467X. Also codon optimized for expression in E.c…PromoterT7Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSFBAD09
Plasmid#11963DepositorInsertSspDnaEC
TagsCBDExpressionBacterialAvailable SinceSept. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpP-T500
Plasmid#153302PurposeExpresses ClpP protease in the cell-free TX-TL systemDepositorInsertclpP (clpP Escherichia coli)
ExpressionBacterialAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTBX-3
Plasmid#26069DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYB-Dual(mNG(yeast-opt))
Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
ExpressionBacterial and YeastPromoterpGAL1(yeast) and pT7-RNAP(bacteria)Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G1
Plasmid#40174DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS3
Plasmid#109287PurposeExpresses proteins in mycobacteriaDepositorTypeEmpty backboneExpressionBacterialMutationNew promoter/cloning siteAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHD_v2
Plasmid#32183DepositorInsertTALE Monomer HD
ExpressionBacterialAvailable SinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNG_v2
Plasmid#32181DepositorInsertTALE Monomer NG
ExpressionBacterialAvailable SinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEB1-coVenNB
Plasmid#104019PurposePlasmid encoding coVenNBDepositorInsertcoVenNB
UseLow copyExpressionBacterialMutationSame protein sequence as Venus NB (codon optimize…PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVHed05
Plasmid#82351PurposeContains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetRDepositorInsertsUseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRC1765
Plasmid#141346PurposeMini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET15-EF1p_mFAP2b
Plasmid#159469PurposeEncodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p_mFAP2b
Tags6xHis-thrombin-TEVExpressionBacterialAvailable SinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBA2257
Plasmid#38151DepositorInsertMBP-proMeCPA fusion protein
Tagsmaltose binding proteinExpressionBacterialPromotertacAvailable SinceAug. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-gfp
Plasmid#128141PurposeDinoflagelate backbone for gfp expressionDepositorInsertGreen fluorescence protein
ExpressionBacterialPromoterDinoflagellate potential promotersAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAT4
Plasmid#112585PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusionsDepositorTypeEmpty backboneTags6HisExpressionBacterialAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
TPMT
Plasmid#25497PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV106
Plasmid#63196PurposepRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
J-HARS-4
Plasmid#166529PurposescFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-SARS-7
Plasmid#166527PurposescFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2-TP53
Plasmid#111089PurposepET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.DepositorInsertTP53 (TP53 Human)
ExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFCyR5
Plasmid#149480PurposeBroad-Host range expression vector, RSF1010 origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPCymRCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mEos3.2-A69T
Plasmid#98571Purposephotoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmEos3.2-A69T
Tags7xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Ets1-ETS
Plasmid#85735PurposeExpresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440DepositorInsertDNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Mouse, Human)
Tags6xHis following thrombin cleavage site LVPR|GExpressionBacterialAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_P_12_asn_mbfA
Plasmid#90289PurposeBB1 promoter mbfA gene of Aspergillus niger between FS1 and FS2DepositorInsertmbfA
ExpressionBacterialAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
ExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-mEGFPBb
Plasmid#118234PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFPDepositorInsertflagellin promoter driven mEGFP
ExpressionBacterialAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Fer-SH2
Plasmid#46425DepositorInsertFer (FER Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 450-558PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ALDH1A1
Plasmid#189749PurposeThe construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.DepositorAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only