32,167 results
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Plasmid#74063PurposepZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanRDepositorInsertdcas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPropionate inducible promoter (prp)Available SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
HYG-TET-OFF – ubiquitin – LEU – 4FLAG
Plasmid#193315PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4FLAG N-terminal tagging
Tags4FLAGExpressionBacterialAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-mNG
Plasmid#189722Purposetitratable expression of mNeonGreen in bacteriaDepositorInsertmNeonGreen
TagsHis-T7-EKExpressionBacterialPromoterpBADAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAH25-SceI
Plasmid#129389PurposeDerived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.DepositorInsertChloramphenicol acetyltransferase
ExpressionBacterialMutationN/APromoterCAT promoterAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0915 (CRISPR with target 3)
Plasmid#130643PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertCRISPR(target-3)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp2-GFPuv
Plasmid#139083PurposeLow phosphate inducible promoter yibDp2 for GFP expressionDepositorInsertyibDp2-GFPuv
ExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAGD289
Plasmid#228113PurposepET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoterDepositorInsertKva1 retron, ncRNA with 90 bp rpoB donor
ExpressionBacterialPromoterT7Available SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSucCD2
Plasmid#83324Purposeexpresses E. coli succinyl-CoA synthetaseDepositorInsertsucCD
ExpressionBacterialAvailable SinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYTK080
Plasmid#65187PurposeEncodes ZeocinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertZeocinR
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His-RGS4
Plasmid#204847PurposeExpress His tagged-RGS4 in bacterial cells for protein purificationDepositorAvailable SinceSept. 14, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRsetB-RoTq-Off
Plasmid#234466PurposeBacterial expression vector for RoTq-OffDepositorInsertRoTq-Off
Tags7xHisExpressionBacterialAvailable SinceMay 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJH643
Plasmid#172168PurposeReporter plasmid for pylBCD variant in pBAD backboneDepositorInsertPara sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
ExpressionBacterialMutationsfGFP(N39*, N135*, Y151*)Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)
Plasmid#29724DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTA-attB
Plasmid#18937DepositorInsertphiC31 attB
ExpressionBacterialAvailable SinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
ALDH18A1
Plasmid#38947PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-PfAgo_F
Plasmid#146265PurposeBacterial Expression of PfAgoDepositorInsertPfAgo
ExpressionBacterialMutationone silent T1704C and one non silent mutation A90…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_RBFOX2-Halo
Plasmid#175304PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHflu4
Plasmid#203882PurposepHI1 with insertion of monomeric red fluorescent protein (mRFP)DepositorInsertmonomeric red fluorescent protein
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
JMJD5
Plasmid#39113PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVS_AF
Plasmid#188950PurposeLevel 2 destination vector containing pBBR1 origin of replication and CIDAR AF scarsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002
Plasmid#228639PurposeBacterial expression of the Coxsackievirus 33 isoform B 3C proteaseDepositorInsertCoxsackievirus 33 isoform B 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a human Tankyrase2 full length Y920E
Plasmid#132639Purposeexpresses human Tankyrase2 full length Y920E in E. coliDepositorInsertTankyrase2 Y920E
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET PPL His6 MBP LIC cloning vector (2K-T)
Plasmid#37183DepositorTypeEmpty backboneTagsHis6, MBP, and PPLExpressionBacterialPromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pE6c
Plasmid#17466Purposemodified pENTR vector for C-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
DUSP16
Plasmid#38918PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only