30,807 results

1. pJJS012

   Plasmid

   #188335

   Purpose

   PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   Linker::mTagBFP2::mIAA7
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b(+)_ALFA-tag-SNAP

   Plasmid

   #136628

   Purpose

   Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-NbALFA
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQexp

   Plasmid

   #25820

   Depositor

   George Church

   Article

   Tolonen et al Mol Microbiol. 2009 Dec . 74(6):1300-13.

   Insert

   pQexp
   

   Expression

   Bacterial

   Available Since

   Sept. 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR4 hNFATc1-EE

   Plasmid

   #17857

   Depositor

   Jerry Crabtree

   Article

   Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

   Insert

   NFATc1 (NFATC1 Human)
   

   Tags

   EE

   Expression

   Bacterial

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pSL4164 (pEffector_IscB,ωRNA)

   Plasmid

   #210874

   Purpose

   Expresses GstIscB and native ωRNA from constitutive promoters

   Depositor

   Samuel H. Sternberg

   Article

   Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

   Insert

   GstIscB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a (+)-Gal4-vp16

   Plasmid

   #171075

   Purpose

   Expresses Gal4-vp16 fusion protein in bacteria

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   Gal4 DBD (NEWENTRY Budding Yeast)
   

   Tags

   His and VP16 AD (413-490)

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExp-IF2

   Plasmid

   #112567

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-IF2 and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKD184

   Plasmid

   #90953

   Purpose

   Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Thiosulfate response regulator
   Superfolder GFP
   mCherry
   

   Expression

   Bacterial

   Promoter

   Bba_J23114, PltetO-1, and PphsA

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ateam1.03-nD/nA/pRSETB

   Plasmid

   #51960

   Purpose

   bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

   Depositor

   Takeharu Nagai

   Article

   Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

   Insert

   ATeam1.03-nD/nA
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. SiriusGFP

    Plasmid

    #123201

    Purpose

    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

    Insert

    SiriusGFP
    

    Use

    Mouse Targeting

    Tags

    N/A

    Expression

    Bacterial and Mammalian

    Mutation

    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDD132

    Plasmid

    #119885

    Purpose

    Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    BbaJ23104_EYFP
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMM627

    Plasmid

    #112532

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    luxCDABE
    

    Expression

    Bacterial

    Promoter

    J23107, PL(HrtO), and ProD

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQTEV-MAT2B

    Plasmid

    #34795

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    methionine adenosyltransferase II, beta (MAT2B Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid

    #178043

    Purpose

    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p403 – TDH3-MTS-mCherry-GFP1-10-His

    Plasmid

    #177730

    Purpose

    mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    mCherry-GFP1-10
    

    Tags

    Mitochondrial Targeting Sequence

    Expression

    Bacterial and Yeast

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. spCas9-NLS-MAV

    Plasmid

    #124212

    Purpose

    SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

    Depositor

    Uri David Akavia

    Article

    Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    His6, MBP, TEV Cleavage site, and two copies of S…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNIC-ZB

    Plasmid

    #26107

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Tags

    His6 - Z-basic - TEV

    Expression

    Bacterial

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-Hsp90 N(9-236)

    Plasmid

    #22481

    Depositor

    William Sessa

    Article

    Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

    Insert

    Hsp90 alpha N domain (HSP90AA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC101-ΔPS-PM,CS-RBSBBa_B0034-geneVI

    Plasmid

    #134356

    Purpose

    Accessory plasmid

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    Gene VI
    

    Expression

    Bacterial

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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