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pGEX-2T PTP-1B

Plasmid

#8602

Depositor

Ben Neel

Insert

PTP-1B (PTPN1 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pBAD-smURFP-RBS-HO-1

Plasmid

#80341

Purpose

Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

Depositor

Erik Rodriguez, Roger Tsien

Publication

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Tags

6 Histidine Tag

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET28b-BE1

Plasmid

#73018

Purpose

Expresses BE1 with N-terminal His6 tag in E. Coli

Depositor

David Liu

Publication

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SpyLigase

Plasmid

#51722

Purpose

Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

Depositor

Mark Howarth

Publication

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SpyLigase

Tags

His6 Tag

Expression

Bacterial

Promoter

T7

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-SrtAdelta59

Plasmid

#51138

Purpose

expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

Depositor

Hidde Ploegh

Publication

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.aureus SrtA delta 59

Tags

6x His

Expression

Bacterial

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pGEX-GST-AGO2

Plasmid

#24317

Depositor

Carl Novina

Publication

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO2 (AGO2 Human)

Tags

GST

Expression

Bacterial

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pMLM36 (aka pBR-UV5-GP-FD2)

Plasmid

#21871

Depositor

Keith Joung

Publication

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWT PolI

Plasmid

#11721

Depositor

Lawrence Loeb

Publication

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I (polA E. coli)

Expression

Bacterial

Mutation

wild-type

Available since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pET21b-Casp1-His

Plasmid

#11809

Depositor

Guy Salvesen

Insert

Caspase-1 (CASP1 Human)

Tags

His

Expression

Bacterial

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDae079 (eMutaT7[transition])

Plasmid

#187622

Purpose

Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Publication

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Inserts

TadA-8e-T7RNAP
PmCDA1-T7RNAP

Expression

Bacterial

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-IPP1-His

Plasmid

#118978

Purpose

Bacterial expression plasmid for Inorganic pyrophosphatase

Depositor

Sebastian Maerkl

Publication

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

inorganic diphosphatase IPP1 (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Publication

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTriEx4-H6-FGAm (FlincG3)

Plasmid

#49202

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Publication

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FlincG3

Tags

His tag + other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Met to Lys in cpEGFP region similar to CGaMP3

Promoter

CMV

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJBEI-6409

Plasmid

#47048

Purpose

BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

Depositor

Taek Soon Lee

Publication

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene

Use

Synthetic Biology; Bglbrick

Expression

Bacterial

Mutation

R364S mutation in MK

Promoter

PlacUV5

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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FLAG-Cdk9

Plasmid

#28100

Depositor

Andrew Rice

Publication

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cdk9 (CDK9 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMV306hsp+Lux

Plasmid

#26159

Depositor

Brian Robertson, Siouxsie Wiles

Publication

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTriEx-PA-Rac1

Plasmid

#22024

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Tags

6X His

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pENTR11 WT NFAT1

Plasmid

#11791

Depositor

Anjana Rao

Insert

NFAT1 (Nfatc2 Mouse)

Tags

HA

Expression

Bacterial

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pPSU150

Plasmid

#173836

Purpose

Expresses 150 kD Penn State ladder protein

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABMBPpepN

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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