34,232 results

Showing: 5661 - 5680 of 34232 results

Results per page:

Sort results by:

pTA-Mob 2.0 Tp

Plasmid

#188619

Purpose

Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

Depositor

Bogumil Karas

Publication

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Conjugation helper plasmid

Expression

Bacterial and Yeast

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJLG039

Plasmid

#192982

Purpose

BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter

Depositor

Clay Fuqua

Publication

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

T7 polymerase

Expression

Bacterial

Promoter

lac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18T-mini-Tn7T-Gm-Pc-tdTomato

Plasmid

#199250

Purpose

Fluorescent labelling of bacterial cells with tdTomato

Depositor

Uwe Mamat

Publication

Mamat et al Appl Environ Microbiol. 2023 May 17:e003

Insert

Codon-optimized tdTomato gene

Expression

Bacterial

Mutation

Codon optimization for Xanthomonadaceae

Promoter

Pc promoter from class III integron of Delftia a…

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
luxA+luxB/pRSETB Duet

Plasmid

#197581

Purpose

Expressing luxA and luxB under dual T7 promoter

Depositor

Takeharu Nagai

Publication

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxB

Tags

T7 tag and 6xHis

Expression

Bacterial

Promoter

T7

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUS250

Plasmid

#198322

Purpose

Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein

Depositor

Nicholas Coleman, Mark Somerville

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

cumate-inducible

Available since

April 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

Enlarge
SupD v2

Plasmid

#198598

Purpose

Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SupD

Expression

Bacterial

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc12-HiBiT

Plasmid

#198308

Purpose

Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc12-HiBiT

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnfΔpvc13

Plasmid

#198283

Purpose

Produces PVCs lacking tail fibers

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc13

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Twin-Strep(R)-Tev-GSGS-Ubiquitin

Plasmid

#186803

Purpose

Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

Depositor

Lalit Deshmukh

Publication

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

Twin-Strep(R)-Tev-GSGS-Ubiquitin

Tags

GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

Expression

Bacterial

Mutation

Strep tag Lysine's mutated to Arginine

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDule2 - Mj Acd A9

Plasmid

#197652

Purpose

E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

Depositor

Ryan Mehl

Publication

Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

Inserts

Mj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA

Tags

None

Expression

Bacterial

Mutation

Y32A, L65D, F108L, Q109E, D158S, L162T

Promoter

GlnS and lpp

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLN-AIDA-Tyr1

Plasmid

#192831

Purpose

IPTG inducible AIDA-Tyrosinase expression

Depositor

André Studart

Publication

Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

Insert

AIDA-Tyr1

Expression

Bacterial

Promoter

pL-lac

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQmod3C-GG

Plasmid

#191350

Purpose

Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Publication

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
His-MBP_G4G6 in pRSF

Plasmid

#188454

Purpose

Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

Depositor

Roberto Dominguez

Publication

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

G4G6 (GSN Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK1008 (pSL7)

Plasmid

#191003

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Publication

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-ALDH1A1

Plasmid

#189749

Purpose

The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

Depositor

Robert Sobol

Publication

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pBA630

Plasmid

#186241

Purpose

RfxCas13d Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Publication

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-RfxCas13d + crRNA negative control

Expression

Bacterial

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBK.079

Plasmid

#187218

Purpose

Expresses Eco1 RT and Cas1 + Cas2

Depositor

Seth Shipman

Publication

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 Reverse Transcriptase
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23115 and T7/lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTPH405c

Plasmid

#185726

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C) single PAM linker

Use

CRISPR

Expression

Bacterial

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBFC0625

Plasmid

#186691

Purpose

VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Publication

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_lacZ_α_1 Spacer crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pN006

Plasmid

#188470

Purpose

Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

Depositor

Le Cong

Publication

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

AsCas12a

Use

Lentiviral

Expression

Bacterial and Mammalian

Available since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge