32,926 results

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pET22b-mEB1-eGFP-6xHis

Plasmid

#130981

Purpose

Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB1-GFP-6xHis (Mapre1 Mouse)

Tags

eGFP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-Sth1Cas9

Plasmid

#129552

Purpose

Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

Depositor

Fong Tian Wong, Mingzi M. Zhang

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

Sth1Cas9

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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ERG_pet28a

Plasmid

#131642

Purpose

Bacterial expression of ETS gene ERG

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ERG (ERG Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Sender plasmid

Plasmid

#127677

Purpose

Encodes the synthase LuxI for the quorum-sensing molecule C6-HSL.

Depositor

Friedrich Simmel

Article

Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

Insert

LuxI

Use

Synthetic Biology

Tags

LVA

Expression

Bacterial

Promoter

pT7

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

Plasmid

#72888

Purpose

fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

Insert

Gaussia luciferase, Channelrhodopsin-2, EYFP

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIKpKan

Plasmid

#129271

Purpose

Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoter

Depositor

Lindsey Burbank

Article

Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-G97 (for GFP)

Plasmid

#130715

Purpose

Plasmid encoding the bacterial expression of LAMA-G97 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-G97

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBT29-splitC

Plasmid

#122598

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeE

Plasmid

#129120

Purpose

pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

PBAD

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1750

Plasmid

#127984

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAC574

Plasmid

#127975

Purpose

AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

hygB (hygromycin resistance marker)

Use

Fungal expression

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pORTMAGE312B

Plasmid

#128969

Purpose

Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E32K

Expression

Bacterial

Mutation

E32K

Promoter

xylS-Pm

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pExp-Skp

Plasmid

#129241

Purpose

Protein expression in E.coli

Depositor

Marko Hyvönen

Type

Empty backbone

Tags

His and Strep

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

Plasmid

#110556

Purpose

pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

Depositor

Martin Pule

Article

Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

Insert

anit-RD114 light chain

Tags

mKappa

Expression

Bacterial and Mammalian

Promoter

EF-1 alpha core promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV801

Plasmid

#126044

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV807

Plasmid

#126600

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP26-1A(sfGFP)

Plasmid

#126973

Purpose

Level 1 vector. sfGFP reporter. Origin p15A (medium copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB1m

Plasmid

#120286

Purpose

Produce human recombinant mTGFB1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

monomeric Transforming Growth Factor-β1

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promotor

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB3m

Plasmid

#120289

Purpose

Produce human recombinant mTGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

monomeric Transforming Growth Factor-β3

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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