30,807 results

1. pET28a-DDX43

   Plasmid

   #134574

   Purpose

   Express human DDX43 protein (WT, full-length) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43 (DDX43 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length WT

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLG017 (AVAST type 1)

   Plasmid

   #157895

   Purpose

   Expresses the AVAST type 1 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   AVAST type 1 (STAND ATPase with N-terminal serine protease)
   

   Expression

   Bacterial

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDonor_Tn-pOUT

   Plasmid

   #213905

   Purpose

   Mariner transposon with outward-facing pJ23104 promoter

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and outward-facine pJ23104 promoter
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FHL-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129628

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   FHL-cpmTq2-Calcium-lifetime-sensor
   

   Tags

   6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

   Expression

   Bacterial and Mammalian

   Promoter

   CMV,&Rhamnose

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSETB-roGFP1-R12

   Plasmid

   #83311

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

   Insert

   roGFP1-R12
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

   Promoter

   T7

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pNM58

   Plasmid

   #128409

   Purpose

   System with AIP and xylose inducible agrCA sensor

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Inserts

   argCA
   gfpmut2
   

   Expression

   Bacterial

   Promoter

   P3 and PxylR

   Available Since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. FnR9-10

   Plasmid

   #182567

   Purpose

   Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 9-10
   

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. T7 promoter + filler DNA reporter plasmid

   Plasmid

   #156454

   Purpose

   A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   NeoR/KanR
   TcR
   

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7 and tet

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFCM1

   Plasmid

   #64948

   Purpose

   source of CmR FRT cassette

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   FRT-cat-FRT
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. ykgMO-IGS-sfGFP

    Plasmid

    #207231

    Purpose

    Calprotectin sensing promoter with sfGFP reporter

    Depositor

    Arthur Prindle

    Article

    Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    ykgMO-IGS
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_mouse-pro-IL18

    Plasmid

    #183389

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (Il18 Mouse)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Biomolecular FeedBack (FB)

    Plasmid

    #109391

    Purpose

    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pJ23100mut-dCas9-T- phtpG1-sgRNA
    

    Expression

    Bacterial

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAH-CTX1-rhadCas9

    Plasmid

    #129391

    Purpose

    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Burkholderia cenocepacia codon-optimized dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Entire dCas9 codon optimized for the GC-rich B. c…

    Promoter

    PrhaBAD

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx-mVenus-Zdk1

    Plasmid

    #81047

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNHEJ-recAmu-sacB

    Plasmid

    #158717

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recA
    

    Expression

    Bacterial

    Mutation

    R61C

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAJT194

    Plasmid

    #202293

    Purpose

    Type IIS cloning plasmid for LPattB5 integrations w/ OriT

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    sfGFP
    

    Expression

    Bacterial

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKK233-hCaM

    Plasmid

    #47598

    Depositor

    Emanuel Strehler

    Article

    Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

    Insert

    CaM (CALM3 Human)
    

    Expression

    Bacterial

    Mutation

    contains 469 bp fragment (nt 92-559)

    Promoter

    trc

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. PJQ49

    Plasmid

    #134657

    Purpose

    Contains Promoter 3 with bsa1 cut sites for inserting sgRNA

    Depositor

    Jason Peters, Oren Rosenberg

    Article

    Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Anderson BBa_J23115

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. A1-LCD deltahexa

    Plasmid

    #169714

    Purpose

    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deleted

    Depositor

    Tanja Mittag

    Article

    Martin et al Science. 2020 Feb 7;367(6478):694-699. d

    Insert

    hnRNPA1 (HNRNPA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hnRNPA1-A Low complexity domain including amino a…

    Promoter

    T7

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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