30,263 results

1. 6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

   Plasmid

   #174424

   Purpose

   Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(Thrombin)EGFP(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET_RGG-RGG-RGG

   Plasmid

   #124938

   Purpose

   Expression of RGG-RGG-RGG

   Depositor

   Benjamin Schuster, Matthew Good, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSJ111

   Plasmid

   #78707

   Purpose

   Modular vector for constitutive secreted amylase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   amyEopt
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-FOXO3a WT

   Plasmid

   #1790

   Depositor

   Michael Greenberg

   Article

   Brunet et al Cell 1999 Mar 19;96(6):857-68.

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-Tight-Puro_Nkx2.2

   Plasmid

   #64840

   Purpose

   A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Nkx2.2 (Nkx2-2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE-Tn7-146

   Plasmid

   #118562

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSW149

   Plasmid

   #122248

   Purpose

   pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

   Depositor

   Sarah Walker

   Article

   Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

   Insert

   TIF4631 (TIF4631 Budding Yeast)
   

   Use

   Tags

   Intein-Chitin-binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-tdMCP-24xGCN4

   Plasmid

   #205157

   Purpose

   express tdMCP-24xGCN4

   Depositor

   Weirui Ma

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   tdMCP-24xGCN4
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWSK129-stm2585

   Plasmid

   #174401

   Purpose

   S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS hTSP-5

    Plasmid

    #13002

    Depositor

    Jack Lawler

    Article

    Newton et al Genomics. 1994 Dec . 24(3):435-9.

    Insert

    COMP (COMP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pCS-PesaRlux

    Plasmid

    #47640

    Purpose

    wt esaR promoter in pCS26

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    PesaR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PesaR

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE-60NA-mCherry2C

    Plasmid

    #160461

    Purpose

    Encodes an mCherry variant that displays lower cytotoxicity in bacteria

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    mCherry2C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. SARS_pNIC-CTB10H

    Plasmid

    #153056

    Purpose

    Expression construct for producing Avi tagged Seryl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    SARS (SARS1 Human)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a_SYK

    Plasmid

    #224240

    Purpose

    Expression of human SYK Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    syk (SYK Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a_LYN

    Plasmid

    #224238

    Purpose

    Expression of human LYN Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    lyn (LYN Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNHA5

    Plasmid

    #223647

    Purpose

    TurboID protein purification

    Depositor

    François-Xavier Campbell-Valois

    Article

    Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

    Insert

    TurboID
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available since

    Oct. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pNHA6

    Plasmid

    #223648

    Purpose

    BioID protein purification

    Depositor

    François-Xavier Campbell-Valois

    Article

    Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

    Insert

    BioID
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available since

    Oct. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pNHA7

    Plasmid

    #223649

    Purpose

    birA protein purification

    Depositor

    François-Xavier Campbell-Valois

    Article

    Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

    Insert

    BioID_G118R
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G118R

    Promoter

    LacI

    Available since

    Oct. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pSR1058

    Plasmid

    #208743

    Purpose

    Characterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Insert

    PHbs
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PHbs

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM-60-NusA-PfAgo_F

    Plasmid

    #146270

    Purpose

    Bacterial Expression of PfAgo

    Depositor

    Elisa Izaurralde

    Insert

    PfAgo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one non silent mutation A907G (I303V) and one sil…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge