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31,059 results

Showing: 5681 - 5700 of 31059 results

  2. pelB-MBP-BB0323-ss

    Plasmid
    #137067
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0323: lacks the BB0323 signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137067-plasmid-map-sequence-id-291957

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  6. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

    Plasmid
    #164614
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    stdMCP-stdHalo-RH1
    Use
    Lentiviral
    Tags
    HA tag and NLS
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164614-plasmid-map-sequence-id-325003

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  7. ITCH

    Plasmid
    #36197
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH (ITCH Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains HECT domain
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

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  10. pET28a FKBP12-V5

    Plasmid
    #194117
    Purpose
    Expresses FKBP12 protein with V5 tag
    Depositor
    Jun Liu
    Insert
    FKBP1A (FKBP1A Human)
    Expression
    Bacterial
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194117-plasmid-map-sequence-id-380734

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  14. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    129391-plasmid-map-sequence-id-273754

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  15. p405TEF1

    Plasmid
    #15968
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15968-plasmid-map-sequence-id-8453

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  18. pDx_mScarlet3-NCwt-SYFP2

    Plasmid
    #189766
    Purpose
    Dual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysis
    Depositor
    Dorus Gadella
    Article
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Insert
    mScarlet3-NCwt-SYFP2
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Mutation
    encodes the expected internal mutations relative …
    Promoter
    CMV & Rha
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189766-plasmid-map-sequence-id-375338

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Showing: 5681 - 5700 of 31059 results