32,826 results
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Plasmid#169015PurposeExpresses GST-p37 (human, codon optimized for expression in bacteria)DepositorInsertp37 (UBXN2B Human)
TagsGSTExpressionBacterialMutationsequence was codon optimized for expression in E.…PromotertacAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL
Plasmid#169105PurposeExpresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXZX353
Plasmid#169457PurposeMATα (alpha) fragment in pTOPO vectorDepositorInsertMatA
ExpressionBacterialPromoterlacAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
POLArIS_NbVim-cp10GFP
Plasmid#171020PurposeExpresses NbVim-cp10GFP#2 in mammalian cellsDepositorInsertanti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
Tagscp10GFPExpressionBacterial and MammalianPromoterCMV promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ1
Plasmid#167130PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterFrr promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS30: pTarget(PmcCAST)
Plasmid#168163PurposeTarget plasmid containing PmcPSP1 and attachment site for PmcCAST.DepositorInsertsPAM for PmcCAST + PmcPSP1
tRNA-Val
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS31: pTwinStrep-SUMO-PmcTnsAB
Plasmid#168164PurposePmcTnsAB expression construct for protein purification.DepositorInsertPmcCAST TnsAB
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD
Plasmid#168153PurposeInducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS17: pTwinStrep-SUMO-AvTnsD
Plasmid#168150PurposeAvTnsD expression construct for protein purification.DepositorInsertAvCAST TnsD
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS18: pHelper(PmcCAST)_PmcPSP1
Plasmid#168151PurposeInducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS15: pTwinStrep-SUMO-AvTnsB
Plasmid#168148PurposeAvTnsB expression construct for protein purification.DepositorInsertAvCAST TnsB
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS16: pTwinStrep-SUMO-AvTnsC
Plasmid#168149PurposeAvTnsC expression construct for protein purification.DepositorInsertAvCAST TnsC
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS14: pTwinStrep-SUMO-AvTnsA
Plasmid#168147PurposeAvTnsA expression construct for protein purification.DepositorInsertAvCAST TnsA
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD
Plasmid#168135PurposeInducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.DepositorInsertsAvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFCiKm7
Plasmid#167521PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.DepositorInsertCinRAM-KmR
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLtR5
Plasmid#167505PurposeBroad-Host range expression vector, RSF1010 origin, TetR repressor, PLtetO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertTetR-RFP
UseSynthetic BiologyExpressionBacterialPromoterPLtetO-1Available SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-7
Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialPromoterP(rhaB)Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)
Plasmid#169168PurposeConjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.DepositorInsertMAP1LC3B (MAP1LC3B Human)
Tags6X Histidine Tag, TEV cleavage site, mCherryExpressionBacterialPromoterT7 lac promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
TagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp15
Plasmid#167265PurposeBacterial expression of Nsp15DepositorInsertNsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
TagsHis6-TEV siteExpressionBacterialMutationnoneAvailable SinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
APneg-SNAP25c
Plasmid#168013Purposenegative selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
APneg-SNAP25b
Plasmid#168012Purposenegative selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pID1072
Plasmid#164869PurposeThe pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.DepositorInsertphiKO2 cB repressor
ExpressionBacterialAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
poRFP-oGFP
Plasmid#154130PurposeExpresses orthogonal gfp and orthogonal rfp genes in bacterial cellsDepositorInsertsorthogonal gfp
orthogonal rfp
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp10_SARS-CoV-2
Plasmid#167851PurposeBacterial expression of the SARS-CoV-2 protein nsp10DepositorInsertnsp10
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)
Plasmid#167268PurposeOverexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coliDepositorInsertHOB
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
ScSpe1 pET-20b(+)
Plasmid#117145PurposeExpresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21DepositorInsertSPE1
TagsHistidineExpressionBacterialPromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-X(5010)B1
Plasmid#168019PurposeExpresses X(5010)B1-6xHis in bacterial cellsDepositorInsertX(5010)B1-6xHis
TagsHisx6ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -