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pSWAP_Entry_H2_beta_CM

Plasmid

#184870

Purpose

pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pNS2-sigmaVL

Plasmid

#26756

Depositor

Michael Elowitz

Article

Dunlop et al Nat Genet. 2008 Dec . 40(12):1493-8.

Insert

CFP, mCherry, and cI-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM Kan + p15A BsaIR Amp

Plasmid

#206208

Purpose

Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM and BsaIR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLysscys255

Plasmid

#36429

Depositor

Pete Lollar, Emine Gunhan

Article

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Use

Tags

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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ZFYVE16

Plasmid

#39099

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ZFYVE16 (ZFYVE16 Human)

Use

Tags

His6-Zb-TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pMTS-hFluc

Plasmid

#177716

Purpose

Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Use

Tags

Mitochondrial Targeting Sequence MTS SS

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHLU

Plasmid

#64167

Purpose

harbours marker genes HIS3, LEU2 and URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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drebrin-His

Plasmid

#40363

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Use

Tags

His and T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pMD1 anti-GFP nanobody-Tn5

Plasmid

#215567

Purpose

Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

Depositor

Isabelle Mansuy

Article

Maria A. et al bioRxiv 2024.07.12.603224

Insert

anti-GFP nanobody-Tn5 fusion

Use

Tags

3xFlag and intein-CBD

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK803

Plasmid

#207138

Purpose

Assembled Tn10 with CP25 promoter driving gfp

Depositor

Nicholas Shikuma

Article

Alker et al ISME Commun. 2023 Sep 19;3(1):98. doi: 1

Insert

Tn10-CP25-GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

CP25

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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mt SSB

Plasmid

#205057

Purpose

SSB1 protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

SSB1 (SSB Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSiM1

Plasmid

#199380

Purpose

Encodes dual cistronic membrane localized GCaMP6s and mScarlet-I optimized for C. elegans expression

Depositor

Hang Lu

Insert

GCaMP6s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX

Use

Tags

Expression

Bacterial

Mutation

no mutations, except added subcellular localizati…

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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p406 – TDH3-Elo3-GFP11-Ura

Plasmid

#177734

Purpose

ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

Elo3 (ELO3 Budding Yeast)

Use

Tags

GFP11 and HDEL

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M337P

Plasmid

#98675

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337P

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M337P

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A326P

Plasmid

#98672

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A326P

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A326P

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDB038a

Plasmid

#99211

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

amber codons at positions 29 and 184 of gIII

Promoter

Ppsp and PproK

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX_hDDX5_FL_D248N

Plasmid

#88872

Purpose

inducible expression of GST-DDX5 D248N in bacteria

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

D248N

Promoter

Tac

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDD87

Plasmid

#119892

Purpose

The pLPD03031 promoter from Rhodococcus opacus PD630 expressing GFP+ on the pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

Insert

pLPD03031_GFP+

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLBH559_Tet-HisCas14a1Locus

Plasmid

#112502

Purpose

Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMyrPalm_mEGFP_IRES_puro2b

Plasmid

#21038

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pMyrPalm

Use

Tags

mEGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only