30,808 results

1. pQE32-pr-mEosFP (mEosFP-V69T)

   Plasmid

   #99213

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEosFP
   

   Expression

   Bacterial

   Mutation

   monomeric variant with mutation V69T for primed c…

   Promoter

   T5

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT100.106b

   Plasmid

   #122601

   Purpose

   clone APOBEC1 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   APOBEC1
   

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH674_pTech_PlldR-HHRz-supP

   Plasmid

   #163728

   Purpose

   Lactate-inducible expression of supP, preceded by a hammerhead ribozyme

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   hhrz-supP
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAWP78-PVCpnf_pvc13-ALFA

   Plasmid

   #198301

   Purpose

   Produces PVCs retargeted with ALFA tag

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-ALFA
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Cre-6xHis

   Plasmid

   #62939

   Purpose

   Expression of Cre-6xHis in bacterial of cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cre recombinase
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD182

   Plasmid

   #90952

   Purpose

   Expresses ThsS under the Ptac promoter

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Insert

   ThsS
   

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTD103aiiA

   Plasmid

   #48884

   Purpose

   Negative feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Danino et al Nature. 2010 Jan 21;463(7279):326-30. do

   Insert

   aiiA and LuxR
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Promoter

   pLux

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

   Plasmid

   #136684

   Purpose

   Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 (BECN1 Human)
   

   Tags

   Trigger Factor

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAF

   Plasmid

   #104152

   Purpose

   Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

   Depositor

   Robert Craigie

   Article

   Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

   Insert

   Barrier-to-autointegration factor (BAF) (BANF1 Human)
   

   Tags

   N-terminal His tag with thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183741

    Purpose

    Expresses Ki67 (8 repeats+LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67 LR domain + 8 Ki-67 repeats

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEutRwc

    Plasmid

    #216334

    Purpose

    An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

    Depositor

    Mark Styczynski

    Article

    McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

    Inserts

    EutR
    Superfolder green fluorescent protein
    

    Expression

    Bacterial

    Available Since

    Dec. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCRISPomyces-AsCas12j-2

    Plasmid

    #191655

    Purpose

    All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

    Depositor

    Fong Tian Wong

    Article

    Tan et al bioRxiv 2021.10.28.465406

    Insert

    AsCas12j-2
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. env8 reverse J1/13

    Plasmid

    #99831

    Purpose

    GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

    Depositor

    Robert Batey

    Article

    Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

    Insert

    env8 reverse J1/13
    

    Tags

    GFPuv

    Expression

    Bacterial

    Mutation

    J1/13 sequence is reversed

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGST2-TSG101 (2-145)

    Plasmid

    #184810

    Purpose

    Bacterial expression of TSG101 UEV domain (aa 2-145)

    Depositor

    James Hurley

    Article

    Im et al Structure. 2010 Nov 10. 18(11):1536-47.

    Insert

    TSG101 (TSG101 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2-145 only

    Promoter

    tac

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNVLTv2-pvdIJD-Prha-base

    Plasmid

    #169244

    Purpose

    Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    rhaRS
    

    Expression

    Bacterial

    Promoter

    rhaS

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD/HisD-PATagRFP

    Plasmid

    #31945

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

    Insert

    PATagRFP
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

    Promoter

    araB

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBJ3

    Plasmid

    #167132

    Purpose

    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    Frr promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFTK055

    Plasmid

    #171327

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    rTA2S-M2 (TetR-3xVP16) transcriptional activator
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT-Hrp48-ADARcd-E488Q-V5

    Plasmid

    #139685

    Purpose

    Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Hrp48, ADAR (Adar Fly)
    

    Tags

    V5

    Expression

    Bacterial and Insect

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM33_USP7_MATH

    Plasmid

    #178490

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    USP7_MATH (USP7 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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