32,417 results
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Plasmid#44423DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only
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pET22B_SA_PcrA
Plasmid#102999PurposeThe plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linkerDepositorInsertSA_PcrA
TagsHis tagExpressionBacterialMutationPcrA 199-2423PromoterT7Available SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promSau/Lysostaphin
Plasmid#125766PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
ExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK constitutive promoterAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET32a-LIC
Plasmid#62310PurposeE. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEVDepositorTypeEmpty backboneTags6xHis, S-tag, and Trx-TagExpressionBacterialPromoterT7-lacOAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE-600n
Plasmid#13537DepositorInsertFLIP-E WT
TagsECFP and VenusExpressionBacterialAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
AK3L1
Plasmid#25541PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTW084b
Plasmid#116894PurposeActivity-independent SE-PACE APDepositorInsertsg3N
434-cI/scFv m3
ExpressionBacterialAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSU313
Plasmid#169695PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
Tags1xFLAG tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS-PesaRlux
Plasmid#47640Purposewt esaR promoter in pCS26DepositorInsertPesaR
UseSynthetic BiologyExpressionBacterialPromoterPesaRAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbS6c-RFP
Plasmid#35293DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterLlacO-1Available SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAGD236
Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
ExpressionBacterialPromoterT7Available SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Dp
Plasmid#79159PurposeExpresses the Dp biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Dp
ExpressionBacterialPromoterT7Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJP_Osc05
Plasmid#229040PurposeLight inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.DepositorInsertpBAD promoter
ExpressionBacterialAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-cAMP
Plasmid#225249PurposeBacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
ExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9_CL
Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyExpressionBacterialPromoterEc-TTL-P112 and Ec-TTL-P104Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEvoltac-EcW-TGA-h14
Plasmid#218764Purposeencodes EcWRS-h14 and EcW-tRNA-TGADepositorInsertEcWRS-h14
ExpressionBacterialMutationS8A, V144G, V146CPromotertacIAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DYNLL1_Dynein-light
Plasmid#110006PurposeProtein expression and purification of DYNLL1_Dynein-lightDepositorInsertDYNLL1_Dynein-light (DYNLL1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only