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pBbB9a-GFP

Plasmid

#126112

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23113

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-HIS2-Vps2

Plasmid

#21494

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

VPS2 (DID4 Budding Yeast)

Use

Tags

6x His and MBP

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
His-drebrin

Plasmid

#40362

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pTHSSd_41

Plasmid

#59964

Purpose

High full length T7 RNAP* expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7* RNAP

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pACYC-GFPM2

Plasmid

#102791

Purpose

Expresses GFP under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-mTagBFP2

Plasmid

#102638

Purpose

In vitro expression of mTagBFP2 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mTagBFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCas_5+7_gfp

Plasmid

#60595

Purpose

encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_5+7_gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCas_2+5

Plasmid

#60594

Purpose

encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_2+5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pNHEJ-Cas12a-recX

Plasmid

#158714

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recX

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Pmyc

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV LIC cloning vector (2O-T)

Plasmid

#29710

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-Mocr-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Cterm_His

Plasmid

#220487

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Nterm_His

Plasmid

#220482

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Cterm_His

Plasmid

#220483

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-RSET-iGABASnFR2n-6xHis

Plasmid

#218886

Purpose

Bacterial expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

Tags

6xHis

Expression

Bacterial

Mutation

S99A F104H R168P

Promoter

T7

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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ISA431: aTc-MP6

Plasmid

#189935

Purpose

Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

danQ926, dam, seqA, emrR, ugi and cda1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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KG#205

Plasmid

#110881

Purpose

Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) D448N cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Use

Tags

Expression

Bacterial

Mutation

changed Asp 448 to Asn

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeA

Plasmid

#128943

Purpose

expresses PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Use

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75)

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-HA-GlpG

Plasmid

#155135

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

tac

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4

Plasmid

#21156

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Apr 9. ():.

Insert

DUX4 (DUX4 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_T2

Plasmid

#184864

Purpose

pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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