30,808 results
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Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p6187
Plasmid#198158Purposebacterial expression of sifA::HaloTag fusion, mutant formDepositorInsertPsifA sifA delta331-336::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
TagsHaloTag-HAExpressionBacterialAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptac-sfGFP
Plasmid#139934PurposeIPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLacI-Ptac-sfGFP
ExpressionBacterialAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-HBB-nLuc
Plasmid#175432PurposeUsed to generate IVT RNA for translation extractsDepositorInsertHBB-nLuc
UseLuciferaseExpressionBacterialPromoterT7Available SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PT7-deGFP-MGapt
Plasmid#67741PurposeT7 promoter expressing deGFP-MG aptamer fusionDepositorInsertUTR1-deGFP-MGapt-T7term
UseSynthetic BiologyExpressionBacterialPromoterPT7Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG_UTu1D
Plasmid#233867PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistantDepositorInsertsallo-tRNA UTu1D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG) and …PromoterEM7, araBAD, and native As SelDAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB117
Plasmid#228481Purpose"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)DepositorTypeEmpty backboneTagsGST (glutathione S-transferase)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET41c-PhTET2
Plasmid#182428PurposeExpresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coliDepositorInsertTetrahedral aminopeptidase TET2 from P. hirokoshii
ExpressionBacterialAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR1H2_Hormone-recep
Plasmid#109827PurposeProtein expression and purification of NR1H2_Hormone-recepDepositorInsertNR1H2_Hormone-recep (NR1H2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AK1
Plasmid#39134PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
Nck
Plasmid#186133PurposeFor expression of recombinant Nck in bacteria cells.DepositorInsertNCK1 (NCK1 Human)
TagsGSTExpressionBacterialMutationAmino acid residues 1-377, with mutations: C139S,…PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7RNAP-Q737P
Plasmid#175356PurposeSupport plasmid for T7_ori plasmidsDepositorInsertT7RNAP-Q737P
ExpressionBacterialMutationQ737PAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmAmetrine1.2
Plasmid#42171DepositorInsertmAmetrine1.2
Tags6HisExpressionBacterialPromoteraraBADAvailable SinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCA24N_6xHis-TEV-rpsA
Plasmid#83585PurposeExpress E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tagDepositorAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcher-sfGFP
Plasmid#107420PurposeFor production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
TagsHis tag, StrepII, and superfolder GFPExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG-RGG
Plasmid#124938PurposeExpression of RGG-RGG-RGGDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
mt deltaN20 TFB2MdeltaC3
Plasmid#205062PurposeTFB2M protein expressionDepositorAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEZY19
Plasmid#18668PurposepET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.DepositorTypeEmpty backboneUseGateway destination vectorTagsHisExpressionBacterialPromoterT7Available SinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD101
Plasmid#131402PurposeE. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD101
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAT205
Plasmid#134882PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only