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Bacterial Expression

30,307 results

Showing: 6241 - 6260 of 30307 results

  5. pHN872

    Plasmid
    #178830
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A
    Use
    Tags
    GST, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178830-plasmid-map-sequence-id-351931

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  6. pTEV5_ RpMatB_2

    Plasmid
    #78206
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do
    Insert
    matB (His6 C106A S170A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A S170A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78206-plasmid-map-sequence-id-146045

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  9. BtuF

    Plasmid
    #92209
    Purpose
    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter
    Depositor
    Douglas Rees
    Article
    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99
    Insert
    BtuF (btuF )
    Use
    Tags
    6x His Tag and PelB Signal Sequence
    Expression
    Bacterial
    Mutation
    BtuF signal sequence removed and uses the PelB si…
    Promoter
    T7 Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92209-plasmid-map-sequence-id-183562

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  15. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

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  16. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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  20. PGL4.11-COL1A1mSMAD

    Plasmid
    #230917
    Purpose
    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant potential SMAD bindin…
    Promoter
    COL1A1
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230917-plasmid-map-sequence-id-458138

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Showing: 6241 - 6260 of 30307 results