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ETV4_pet28a

Plasmid

#131645

Purpose

Bacterial expression of ETS gene ETV4

Depositor

Peter Hollenhorst

Publication

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV4 (ETV4 Human)

Tags

6xHis

Expression

Bacterial

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pgRNA-guideless

Plasmid

#129464

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Publication

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Guideless gRNA backbone

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Ub-GyrA

Plasmid

#128737

Purpose

Expresses Ub fused to GyrA intein at the C-terminal

Depositor

Sharon Rozovsky

Publication

Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

Insert

UBC

Tags

Mxe GyrA

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCAD39_mIRG1_4-462_pvp008

Plasmid

#124873

Purpose

Expresses mouse CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Publication

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (Acod1 Mouse)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 463-end.

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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(SIM)5

Plasmid

#126947

Purpose

Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Publication

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)

Expression

Bacterial

Promoter

unknown

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0374 (His-MBP-Cas8_Cas7_Cas6)

Plasmid

#130639

Purpose

Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

Depositor

Samuel H. Sternberg

Publication

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on Cas8

Expression

Bacterial

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-(SIM)1

Plasmid

#127094

Purpose

Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Publication

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

mCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Tags

mCherry

Expression

Bacterial

Promoter

unknown

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pPEPX

Plasmid

#122631

Purpose

BglFusion compatible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Publication

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pASR103

Plasmid

#122637

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Publication

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-GST-RNMT

Plasmid

#112709

Purpose

Expresses N-terminally GST-tagged human RNMT in bacterial cells

Depositor

Daniel Schoenberg

Publication

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

GST and thrombin cleavage site

Expression

Bacterial

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSM019

Plasmid

#128291

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Publication

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-sfyfp-parB

Expression

Bacterial

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV812

Plasmid

#126605

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV809

Plasmid

#126602

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV804

Plasmid

#126597

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV806

Plasmid

#126606

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSR348

Plasmid

#124713

Purpose

Expression of narX under Ptac

Depositor

Jeffrey Tabor

Publication

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narX (narX )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S193* - LacI is truncated and likely deactivated.…

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Publication

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarR2

Plasmid

#112813

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdTomato

Depositor

Chun Han

Publication

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1A(sfGFP)

Plasmid

#126960

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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