32,934 results

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HBT-GCaMP6-HA

Plasmid

#107433

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

GCaMP6S

Tags

HA

Expression

Bacterial

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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B1-4

Plasmid

#111466

Purpose

translational elongation factor Tu

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor Tu

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDJSVT89

Plasmid

#109303

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

TamA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 NEDD4L WW domain #2

Plasmid

#104205

Purpose

Bacterial expression of WW domain #2 from NEDD4L

Depositor

Sachdev Sidhu, Marius Sudol

Insert

NEDD4L WW domain #2 (NEDD4L Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pHAT4

Plasmid

#112585

Purpose

Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His

Expression

Bacterial

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AC

Plasmid

#108686

Purpose

Plasmid for assembly of donor DNA templates consisting of two expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-His-hCE

Plasmid

#112710

Purpose

Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNGTT (RNGTT Human)

Tags

6xHis and Stop codons placed after RNGTT sequence…

Expression

Bacterial

Mutation

R594H

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-FRT

Plasmid

#113398

Purpose

expresses gRNA for Cas9 FRT targetting

Depositor

Jan Michiels

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

exo, beta, gam, sgRNA-FRT

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-10080.1

Plasmid

#111410

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 10080 bases in length

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-ACKN

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-5544.1

Plasmid

#111405

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-GH'

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.944

Plasmid

#108523

Purpose

PCin-YFP Reporter. OHC14 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCin-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1 sortase

Plasmid

#106288

Purpose

Expresses sortase in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

sortase A pentamutant

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGEXNKI-his-GST-3C-LIC

Plasmid

#108709

Purpose

Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-6xHis-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA

Plasmid

#110203

Purpose

Recipient vector for Cranial Neural Crest-specific expression of protein of interest

Depositor

Tatjana Sauka-Spengler

Insert

enhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA

Tags

eGFP

Expression

Bacterial

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.713

Plasmid

#108514

Purpose

PLuxB-YFP Reporter. OC6 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PLuxB-YFP

Expression

Bacterial

Available Since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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B7-5

Plasmid

#111493

Purpose

translational initiation factor 1

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 1

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B7-4

Plasmid

#111492

Purpose

Met-tRNA formyltransferase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Met-tRNA formyltransferase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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Human USP30 (64-502, construct 13, MG-26-82)

Plasmid

#110746

Purpose

Bacterial expression of human USP30 (construct 13)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Mutation

F348D, M350S, I353E + insertion deletion: 64-178;…

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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KG#121

Plasmid

#110879

Purpose

Expresses the C. elegans unc-31 cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

rab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Mutation

Missing first 408bp

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Kan)

Plasmid

#110076

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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