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pcDNA3-HA-human OCRL

Plasmid

#22207

Depositor

Pietro De Camilli

Publication

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Oculocerebrorenal syndrome of Lowe (OCRL Human)

Use

Retroviral

Tags

HA

Expression

Bacterial and Mammalian

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pH3U3-zif268 (omega)

Plasmid

#18046

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

zif268 binding site

Expression

Bacterial

Mutation

The preferred binding site for zif268 is inserted…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Type

Empty backbone

Tags

6xHis, T7 tag, Xpress tag, mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Publication

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET_RTX-6xHis

Plasmid

#145029

Purpose

pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

Depositor

Andrew Ellington

Publication

Bhadra et al BioProtocol 2021

Insert

RTX

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET29b-IPP1-His

Plasmid

#124137

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Inorganic pyrophosphatase (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Publication

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CMV-FlicR1

Plasmid

#74142

Purpose

Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

Depositor

Robert Campbell

Publication

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FlicR1

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mCardinal-pBAD

Plasmid

#54800

Purpose

Localization: Protein Expression Vector , Excitation: 604, Emission: 659

Depositor

Michael Davidson

Publication

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

6xHis and mCardinal

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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Pet28a_Synthetic_Human_H2A.1

Plasmid

#42634

Depositor

Joe Landry

Insert

Human H2A.1 (H2AC13 Human, Synthetic)

Tags

Untagged

Expression

Bacterial

Mutation

Codon Optimized

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pBAD/HisB-PAmCherry1

Plasmid

#31931

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

His

Expression

Bacterial

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

araB

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pOPINF

Plasmid

#26042

Depositor

Ray Owens

Publication

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

His3C-lacZ

Tags

MAHHHHHHSSGLEVL

Expression

Bacterial, Insect, and Mamm…

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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p406ADH1

Plasmid

#15974

Depositor

Nicolas Buchler, Fred Cross

Insert

ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

ADH1 promoter is between SacI and XbaI restrictio…

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET32a-HD46Q

Plasmid

#11515

Depositor

Pamela Bjorkman

Publication

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 46Q glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 46 glutamines, vector m…

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHN924

Plasmid

#178885

Purpose

Expression of GST-His8-HRV3C

Depositor

Kazuhiro Abe

Insert

human rhinovirus 3C protease

Tags

GST, His8

Expression

Bacterial

Promoter

T7

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKAR2-Br512

Plasmid

#161875

Purpose

Expresses Br512 polymerase in E.coli

Depositor

Andrew Ellington

Publication

Maranhao et al medRxiv

Insert

Modified DNA Polymerase I Large Fragment (Br512)

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7, tetPA

Available since

Nov. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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PCV-Cas9

Plasmid

#123643

Purpose

Expresses Cas9 with an N-terminal HUH-tag called PCV2

Depositor

Wendy Gordon

Publication

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Tags

(EAAAK)8 linker and His6 + SUMO

Expression

Bacterial

Mutation

2-116

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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phage UbiC tagRFP-T-DDX6

Plasmid

#119947

Purpose

Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

Depositor

Jeffrey Chao

Publication

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

DDX6 (DDX6 Human)

Use

Lentiviral

Tags

tagRFP-T

Expression

Bacterial and Mammalian

Promoter

UbiC

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pACYC-Taq

Plasmid

#102793

Purpose

Expresses Taq DNA polymerase under WT T7 promoter

Depositor

Andrew Ellington

Publication

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pKM444

Plasmid

#108319

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor

Expression

Bacterial

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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