32,457 results
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Plasmid#199769PurposeExpresses split-intein fused MSP26 for circularization during expression (internal His-Tag)DepositorInsertcMSP26
TagsHis6-TagExpressionBacterialMutationinternal His-TagAvailable SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-protein
Plasmid#161788PurposeRhamnose-inducible expression of 8xHIS-ABE8e in E. coliDepositorInsertABE8e base editor
Tags8xHIS and BPNLSExpressionBacterialPromoterpRhaBADAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEF2
Plasmid#43971DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastPromoterTEFAvailable SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJA5 H-2K(b)
Plasmid#180441PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2K(b)
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-8
Plasmid#228990PurposeFor bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-8
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQE30-HyPer7
Plasmid#141071PurposeBacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
TagsHisx6ExpressionBacterialPromoterT5Available SinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTCF120 H-2D(b)
Plasmid#180454PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b)
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-h-cGAS
Plasmid#127161Purposeexpression of human cGAS proteinDepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5409
Plasmid#132662PurposeRSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
ExpressionBacterialPromoterPaadA and PconIIAvailable SinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSmartBAC-S
Plasmid#107268PurposeBroad host range bacterial artificial chromosome vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-ultraID
Plasmid#172879Purposeexpresses ultraID in E. coliDepositorInsertultraID
TagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Plac-dCas9 (Plac)
Plasmid#105235PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosaDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599AAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Gm
Plasmid#105232PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosaDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attR3
Plasmid#213054PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3DepositorInsertsgRNA
ExpressionBacterialAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT87
Plasmid#109302PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tagDepositorTypeEmpty backboneTagsOmpA 1-27, 6HisExpressionBacterialPromoterT7Available SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p46Cpf1-OP2
Plasmid#98592PurposeProduces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.DepositorInsertFnCpf1
UseCRISPRExpressionBacterialMutationCodon optimized for E. coliPromoteraraBADAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Nano
Plasmid#60409PurposeE. coli expression of SspB NanoDepositorInsertSspB nano
TagsHis-MBP-TEVExpressionBacterialMutationY11K and A15EAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRKH3-ermGFP
Plasmid#27169DepositorInserterythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
ExpressionBacterialMutationpresence of an additional 183-bp region at N term…Available SinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-ALFA-TwinStrep
Plasmid#240248PurposeArabinose-inducible plasmid with ALFA and Twin-Strep tagsDepositorTypeEmpty backboneTags10x HIS, ALFA, Thrombin, and Twin-StrepExpressionBacterialPromoteraraBADAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only