30,733 results

1. Annexin A7-TEV-TwinStrep

   Plasmid

   #198635

   Purpose

   Annexin A7 fibrillization and lipid binding

   Depositor

   Lalit Deshmukh

   Article

   Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

   Insert

   Annexin A7 (ANXA7 Human)
   

   Tags

   TEV-GS-Dual Strep

   Expression

   Bacterial

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet-LahT150

   Plasmid

   #166785

   Purpose

   Cleavage of double glycine motif in peptides to remove the leader sequence

   Depositor

   Wilfred van der Donk

   Article

   Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

   Insert

   LahT150
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSC101-AmpR EV

   Plasmid

   #208184

   Purpose

   empty plasmid vector, non-fluorescent

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Type

   Empty backbone

   Use

   Reporter

   Expression

   Bacterial

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available Since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNCS mCherry

   Plasmid

   #91769

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Cherry
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Promoter

   T7

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MZB209

   Plasmid

   #228492

   Purpose

   BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

   Depositor

   Richard Baker

   Insert

   BirA (birA )
   

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBHRSF184

   Plasmid

   #89482

   Purpose

   Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

   Depositor

   Hideo Iwai

   Article

   Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

   Insert

   Asparaginyl endopeptidase from Oldenlandia affinis
   

   Tags

   Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBad-CA

   Plasmid

   #105665

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBad-HisB-RRvT

   Plasmid

   #87364

   Purpose

   Expresses RRvT, a tandem heterodimer red fluorescent protein

   Depositor

   Robert Campbell

   Article

   Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

   Insert

   RRvT
   

   Tags

   His-Tag

   Expression

   Bacterial

   Promoter

   pBad (Arabinose)

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. eSrtA(4S-9) pET29b

    Plasmid

    #75146

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    N98D S102C A104V A118T F122A K134R F144L I182V E1…

    Promoter

    T7

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJL1 sortase

    Plasmid

    #106288

    Purpose

    Expresses sortase in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    sortase A pentamutant
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE-80L-V4-ELP-EEP13

    Plasmid

    #237223

    Purpose

    Expresses micelle-forming elastin-like polypeptide (ELP)-endosomal escape peptide (EEP) fusion construct in bacteria

    Depositor

    Elliot Chaikof

    Article

    Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

    Insert

    V4-ELP-EEP13
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDG02583

    Plasmid

    #104129

    Purpose

    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    NEDD8-specific protease 1 (NEDP1)
    

    Tags

    14x Histidine tag, Maltose binding protein, and S…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBB540

    Plasmid

    #27393

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    grpE, clpB
    

    Expression

    Bacterial

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBB542

    Plasmid

    #27395

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    dnaK, dnaJ, groESL
    

    Expression

    Bacterial

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-gp32-H6

    Plasmid

    #163912

    Purpose

    Expresses T4 gp32 for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    gp32
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. prhaBAD-anti-mouse-Nb-Hia5

    Plasmid

    #239626

    Purpose

    Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

    Insert

    Hia5
    

    Tags

    6HIS, MBP, TEV, and anti-Mouse Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available Since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET Nucleolin

    Plasmid

    #13037

    Depositor

    Peter Newburger

    Article

    Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

    Insert

    Nucleolin (NCL Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

    Plasmid

    #218860

    Purpose

    10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCG-ICAM(1235)-nrxn1b
    

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. elavl3:hArr-TEV;he1.1:YFP

    Plasmid

    #218855

    Purpose

    D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hArr
    

    Tags

    human Arrestin-Tobbaco Etch Virus protease fusion…

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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