30,307 results

1. pBbE5a-RFP

   Plasmid

   #35277

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLK

   Plasmid

   #64186

   Purpose

   harbours marker genes LEU2 and LYS2

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 8 pCOLI_A_GST

   Plasmid

   #160150

   Purpose

   N-term GST 3C cleavable Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNR64

   Plasmid

   #79540

   Purpose

   Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   

   Use

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACAT1

   Plasmid

   #25510

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ACAT1 (ACAT1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 30, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pGEM p21

   Plasmid

   #8443

   Depositor

   Frederic Mushinski

   Article

   Huppi et al Oncogene 1994 Oct;9(10):3017-20.

   Insert

   Mouse p21 (Cdkn1a Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

7. DT044A

   Plasmid

   #159405

   Purpose

   Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO promoter

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-Himar-dCas9

   Plasmid

   #137079

   Purpose

   Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

   Plasmid

   #122083

   Purpose

   Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII-V5-LaminB1
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of LaminB1

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJS007

    Plasmid

    #188330

    Purpose

    PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    mIAA7::wrmScarlet::Linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSW002-PpsbA_mTurquoise2

    Plasmid

    #108237

    Purpose

    Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    PsbA promoter - mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBbS2a-RFP

    Plasmid

    #35328

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-4T-2/hGGA3(GAT)

    Plasmid

    #79436

    Purpose

    Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

    Depositor

    Kazuhisa Nakayama

    Article

    Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

    Insert

    GGA3 (GGA3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 107-286

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T1-14-3-3 gamma GST

    Plasmid

    #13280

    Depositor

    Michael Yaffe

    Article

    Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pB2Hopt

    Plasmid

    #163830

    Purpose

    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B

    Depositor

    Jerome Fox

    Insert

    SpecR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pro1;ProD;pLacZ-Opt

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLZL2386

    Plasmid

    #48682

    Purpose

    pET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)

    Depositor

    Suzanne Sandmeyer

    Article

    Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

    Insert

    PFV Integrase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEERM1 Km

    Plasmid

    #64024

    Purpose

    Plasmid for integration into psbA2 site of Synechocystis 6803

    Depositor

    Pia Lindberg

    Article

    Englund et al ACS Synth Biol. 2015 Jul 13.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PpsbA2

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AdML-M3

    Plasmid

    #11244

    Depositor

    Robin Reed

    Article

    Zhou et al Proc Natl Acad Sci U S A. 2002 Sep 17. 9

    Insert

    AdML-M3
    

    Use

    Tags

    3 MS2 hairpins

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP

    Plasmid

    #134357

    Purpose

    Reporter plasmid

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Inserts

    GFP
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-mF(3230)A3

    Plasmid

    #168016

    Purpose

    Expresses MBP-F(3230)A3-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-F(3230)A3-6xHis
    

    Use

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge