30,808 results

1. pUC18T-mini-Tn7T-Tp-eyfp

   Plasmid

   #65035

   Purpose

   TpR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   eyfp
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLI1_pet28a

   Plasmid

   #131641

   Purpose

   Bacterial expression of ETS gene FLI1

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   FLI1 (FLI1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGI3EM22C

   Plasmid

   #135488

   Purpose

   Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr LifeAct-tdTomato
   

   Tags

   LifeAct

   Expression

   Bacterial and Yeast

   Promoter

   SpunH2A/Bpr (divergent)

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLxVi5

   Plasmid

   #167516

   Purpose

   Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, Violacein, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-Violacein
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLuxB

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPARA2

   Plasmid

   #200417

   Purpose

   Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)

   Depositor

   Kamil Witek

   Article

   Lesley et al bioRxiv

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Affibody HER2:243.BirA*-pET301/NT-DEST

   Plasmid

   #174692

   Purpose

   Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

   Depositor

   Jean Gariepy

   Article

   Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

   Insert

   Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
   

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   R118G mutation in BirA

   Promoter

   T7 promoter

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH6HTC_HSPA5-Halo

   Plasmid

   #175326

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HSPA5 (HSPA5 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbB6c-GFP

   Plasmid

   #35356

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   LlacO-1

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOP5GP

   Plasmid

   #112610

   Purpose

   Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8His-GST and Avi

   Expression

   Bacterial

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT588

    Plasmid

    #68907

    Purpose

    NanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Expression

    Bacterial

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDual-eGFP(Y203)

    Plasmid

    #63559

    Purpose

    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(Y203)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed threonine at position 203 with respect to…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pTG-mTDG

    Plasmid

    #52266

    Purpose

    Bacterial expression of mouse TDG with C-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    TDG (Tdg Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB-His-MBP-mGSDMD

    Plasmid

    #123365

    Purpose

    Expression of full-length mouse GSDMD in E. coli

    Depositor

    Hao Wu

    Article

    Orning et al Science. 2018 Nov 30;362(6418):1064-1069

    Insert

    GSDMD (Gsdmd Mouse)
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-2T_PV1_VP1

    Plasmid

    #202071

    Purpose

    Bacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    PV1 VP1 protein with Histag
    

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEND-418_Cacnes_LysA

    Plasmid

    #225607

    Purpose

    Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

    Plasmid

    #211115

    Purpose

    For tagging the C-term of Top2A with Venus

    Depositor

    Job Dekker

    Article

    Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

    Inserts

    5' C-term Top2A homology arm (TOP2A Human)
    Venus
    T2A
    Blastocidin Resistance
    5' C-term Top2A homology arm (TOP2A Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    n/a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. KTK_002

    Plasmid

    #180527

    Purpose

    Entry vector containing J23104 promoter

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    J23104 promoter
    

    Expression

    Bacterial

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-2xMreBN-mCherry-His

    Plasmid

    #133626

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    2xMreB-mCherry-His
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLRD14

    Plasmid

    #208266

    Purpose

    Production of syn-pimara-7,15-diene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    osCPS4
    osKSL4
    

    Expression

    Bacterial

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDonorGFP(Ptr)

    Plasmid

    #175578

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    Mini-Tn, GFP cassette
    

    Expression

    Bacterial

    Mutation

    NO

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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