30,808 results

1. pGEX Smad3

   Plasmid

   #12630

   Depositor

   Rik Derynck

   Article

   Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 10, 2006

   Availability

   Academic Institutions and Nonprofits only

2. PAS528 (TJF059)

   Plasmid

   #65602

   Purpose

   pETDuet-1 Containing FadD-His

   Depositor

   Pamela Silver

   Article

   Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

   Insert

   FadD
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7 lacO

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

3. NCD-GFP

   Plasmid

   #24507

   Depositor

   Ron Vale

   Article

   Case et al Cell. 1997 Sep 5. 90(5):959-66.

   Insert

   NCD (ncd Fly)
   

   Tags

   GFP and His

   Expression

   Bacterial

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. Human RNF144B (27-236)

   Plasmid

   #110753

   Purpose

   Bacterial Expression of RNF144B RBR domain

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

   Insert

   RNF144B (RNF144B Human)
   

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_ACTG1_Actin

   Plasmid

   #109989

   Purpose

   Protein expression and purification of ACTG1_Actin

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   ACTG1_Actin
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T-Cdc42-wt

   Plasmid

   #12969

   Depositor

   Gary Bokoch

   Insert

   Cdc42 (CDC42 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-4T2-Rheb

   Plasmid

   #15889

   Depositor

   John Blenis

   Article

   Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

   Insert

   Rheb (RHEB Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

8. 6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

   Plasmid

   #174424

   Purpose

   Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   6xHis(Thrombin)EGFP(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSHDY-PL03-mVenus_tetR

   Plasmid

   #137663

   Purpose

   Conjugative vector derived from pSHDY. Constitutive expression of the repressor tetR, as well as aTc-inducible expression of mVenus.

   Depositor

   Ilka Axmann

   Article

   Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

   Insert

   PL03:mVenus
   

   Expression

   Bacterial

   Promoter

   PL03

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX TIF1gamma

    Plasmid

    #15734

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    TIF1 gamma (TRIM33 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pGEX 4T1 hBad

    Plasmid

    #35567

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    bad (BAD Human)
    

    Tags

    Flag

    Expression

    Bacterial

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pET-His6-Sumo-CfaC-MBP

    Plasmid

    #126018

    Purpose

    Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

    Depositor

    Thomas Muir

    Insert

    His6-Sumo-CfaC-MBP
    

    Tags

    6xHis-SUMO and HA-MBP

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG019

    Plasmid

    #126502

    Purpose

    Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

    Depositor

    Ute Romling

    Article

    Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

    Insert

    disaggregase ClpG with 6xHis-tag
    

    Tags

    C-terminal 6xHis

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE60 R124K wgMDH

    Plasmid

    #204258

    Purpose

    Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124K

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE60 R124Q wgMDH

    Plasmid

    #204259

    Purpose

    Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124Q

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE60 R130E wgMDH

    Plasmid

    #204267

    Purpose

    Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130E

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

    Plasmid

    #99224

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos3.2
    

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pECTO

    Plasmid

    #67683

    Purpose

    pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureus

    Depositor

    Philippe Bouloc

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSHS212

    Plasmid

    #87346

    Purpose

    Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCPP5616

    Plasmid

    #128727

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    avrPtoB
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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