30,307 results

1. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

   Plasmid

   #108693

   Purpose

   For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Inserts

   RNASEH2B (RNASEH2B Human)
   RNASEH2C (RNASEH2C Human)
   RNASEH2A-D34A/D169A
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Shine Dalgarno sequence upstream of start codon, …

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWaldo-GFPe-SH1446

   Plasmid

   #112507

   Purpose

   Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

   Depositor

   Simon Newstead

   Article

   Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

   Insert

   POT family transporter
   

   Use

   Tags

   C terminal TEV GFP octa-His affinity tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPerimCh

   Plasmid

   #227013

   Purpose

   Expresses periplasmic mCherry

   Depositor

   Suzan Rooijakkers

   Article

   Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

   Insert

   PelB-mCherry, arabinose inducible cytosolic GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsM and pBAD

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. evoFERNY-Cas9NG

   Plasmid

   #203329

   Purpose

   Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   evoFERNY-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nsp3 Mac1 - P43 (Oxford)

   Plasmid

   #169210

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSR62

   Plasmid

   #125076

   Purpose

   Expression of ompR under PLtetO-1, output promoter PompF146

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   ompR (ompR )
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. K84M kinase-dead Nuak1-3xflag

   Plasmid

   #97219

   Purpose

   Plasmid expressing kinase-dead mutant Nuak1

   Depositor

   Huda Zoghbi

   Article

   Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

   Insert

   Nuak1 (NUAK1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Kinase-dead K84M

   Promoter

   T7

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSG25-pBAD-tetO-deGFPssrA

   Plasmid

   #102452

   Purpose

   Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   deGFP
   

   Use

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD-tetO

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbB6k-GFP

   Plasmid

   #35357

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LlacO-1

   Available since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_NR2F2_Hormone-recep

    Plasmid

    #109826

    Purpose

    Protein expression and purification of NR2F2_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR2F2_Hormone-recep (NR2F2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. poLuc1

    Plasmid

    #154128

    Purpose

    Expresses orthogonal firefly luciferase gene in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

    Insert

    orthogonal firefly luciferase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIA1385

    Plasmid

    #166862

    Purpose

    Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-GFP-MCC

    Plasmid

    #133307

    Purpose

    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    microcin-V bacteriocin cassette (cvaC E. coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N112D (please see depositors comments)

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSK+ chickBMPR-1B DN

    Plasmid

    #49529

    Purpose

    cloning vector for dominant negative chick BMP receptor 1B

    Depositor

    Peter ten Dijke, Lee Niswander

    Article

    Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

    Insert

    BMPR-1B DN (BMPR1B Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    dominant negative mutation (K231R); also P2A, M11…

    Promoter

    T3

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

15. BB1_12_pGAP

    Plasmid

    #98498

    Purpose

    BB1 with insert: promoter PGAP

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    GAP promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pstb-LAFR5

    Plasmid

    #86169

    Purpose

    pLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid vector with double cos sites.

    Depositor

    Kay Jones

    Article

    Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SMb21651 promoter

    Available since

    Feb. 6, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBTB-3

    Plasmid

    #22819

    Depositor

    Ryan Gill

    Article

    Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBBR1-kan-Ptet-plu-gamma-ET_rhi145

    Plasmid

    #180427

    Purpose

    Genome engineering of Agrobacterium

    Depositor

    Jun Fu

    Article

    Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

    Insert

    plu-gamma-ET_rhi145
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCri-1b

    Plasmid

    #61327

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and MBP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pRS415-SPT6

    Plasmid

    #10993

    Depositor

    Francisco Malagon

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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