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Plasmid#65190PurposeEncodes AmpR-ColE1 as a Type 8 part to be used in the Dueber YTK systemDepositorInsertAmpR-ColE1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Smurf1 wt
Plasmid#13502DepositorInsertSMURF1 (SMURF1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGM190oriT
Plasmid#115679PurposeE. coli-Streptomyces shuttle vector for gene expressionDepositorInsertoriT, aphII, tsr
UseTagsExpressionBacterialMutationPromotertipAAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU398
Plasmid#178980Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)DepositorInsertgRAMP
UseTagsStrep-Tag II SUMOExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRT7 NT Topo arabinose
Plasmid#73446PurposeUsed for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptidesDepositorTypeEmpty backboneUseTagsGST (N-terminal), 6xHis (C-terminal)ExpressionBacterialMutationPromoterpBadAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSG320
Plasmid#115597Purposesurface-displayed Nb2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5085
Plasmid#85122PurposePkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.DepositorInsertPkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
UseTagsECFPExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP1187
Plasmid#119256Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
XOR gate-V2.0
Plasmid#44453DepositorInsertXOR-gate
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet16
Plasmid#41684DepositorInsertNpuDnaE intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEQ111m
Plasmid#40190DepositorInsertEcoRI endonuclease & EcoRI methylase
UseTagsExpressionBacterialMutationchanged Glutamic Acid 111 to GlutaminePromoterLambda PLAvailable sinceNov. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertapmA (apmA Staphylococcus aureus)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_Z17-ehaA_LacI
Plasmid#186313PurposePlasmid for inducible E coli expression of the Z17 helixCAM.DepositorInsertZ17-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas9-recX
Plasmid#158715PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recXDepositorInsertcas9
UseCRISPRTagsExpressionBacterialMutationPromoterTetOAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pETM6-4F2-mCherry
Plasmid#73421PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.DepositorInsertPromoter 4F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP4F2 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc
Plasmid#108234PurposeBroad host bacterial expression vector with constitutive Pc promoter.DepositorInsertPc promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only