32,838 results
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Plasmid#159548PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D
Plasmid#159569PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12DDepositorInsertHis6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D (KRAS Human)
TagsHis6-MBP-tev-AviExpressionBacterialMutationG12DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D
Plasmid#159543PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G13D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG13DAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X10-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V
Plasmid#159542PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12VDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12V (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12VAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZmUbi-5'UTR
Plasmid#154057PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)DepositorInsertPromoter and 5' UTR Ubiquitin (Zea mays)
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL12-GCaMP6f-pHuji bb118
Plasmid#158981PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.DepositorInsertGCaMP6f-pHuji
ExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833 a…PromoterBba_J23118 (sigma 70)Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
eNS2B47NS3 L30SF31S
Plasmid#155319PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 L30SF31S
Plasmid#155316PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2042.2
Plasmid#140670Purpose3-color stripeDepositorInsert3-color stripe
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdUA-lacIN
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…ExpressionBacterialPromoterEscherichia coli lacIN promoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp2-GFPuv
Plasmid#139083PurposeLow phosphate inducible promoter yibDp2 for GFP expressionDepositorInsertyibDp2-GFPuv
ExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC060-HisMS2_PLP_Env_pac
Plasmid#155040PurposeGeneration of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope GeneDepositorInsertsMaturation Protein
Coat Protein Dimer
Envelope Gene
ExpressionBacterialMutationRemove TypeIIs Restriction SitesPromoterT7Available SinceSept. 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBJG1 T7-8xHis-OGT 5N5A
Plasmid#154280PurposeBacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationN322A, N356A, N390A, N424A, N458A mutations; bact…Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HA-GlpG
Plasmid#155135PurposeExpresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1DepositorAvailable SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
PET.SUMO eIF4G1 1-200
Plasmid#158791PurposeBacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.DepositorInserteIF4G1 (codon optimized)
Tags6xHIS and SUMOExpressionBacterialMutationamino acids 1-200PromoterT7Available SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP378
Plasmid#158797PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purificationDepositorInsertdCasPhi-1 (D371A)
ExpressionBacterialAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-UAP56
Plasmid#157661PurposeExpresses UAP56 in E.coli cellDepositorInsertSpliceosome RNA helicase DDX39B (DDX39B Human)
TagsGST tagExpressionBacterialMutationdeleted amino acids 1-43Available SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCD564
Plasmid#153026PurposedxCas9(3.7), MCP-SoxS(R93A/S101A)DepositorInsertdxCas9(3.7), MCP-SoxS(R93A/S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsAvailable SinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-tVYN-Nt∆
Plasmid#154431PurposePlasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detectionDepositorInsertVenusΔC12-NtYcgRΔ-NLucΔ4
ExpressionBacterialPromoterT7Available SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2-77 pComb3xss anti-cFos nanobody
Plasmid#145834PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody S2-77 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo1
Plasmid#157805PurposeExpression of synthetic protein composed of eGFP and BRD4 bromodomain #1DepositorInserteGFP-bromo1
TagsHis6 and MBPExpressionBacterialPromoterpTACAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
C2-82 pComb3xss anti-cFos nanobody
Plasmid#145838PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C2-82 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1-71 pComb3xss anti-cFos nanobody
Plasmid#145832PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody S1-71 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluf
Plasmid#119833PurposeProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluf
TagsGSTExpressionBacterialMutationE25DPromoterT7Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-LANA
Plasmid#157801PurposeExpression of mEGFP and LANA peptide fusion proteinDepositorInsertmEGFP-LANA
TagsHis6 and MBPExpressionBacterialPromoterpTACAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3a_Ub1
Plasmid#156467PurposeBacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tagDepositorInsertNsp3a_Ub1 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf10
Plasmid#156486PurposeBacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tagDepositorInsertOrf10 (ORF10 Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19/DdmEGFP
Plasmid#154304Purposecloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideumDepositorInsertmEGFP
ExpressionBacterialAvailable SinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mlcR25-mEGFP
Plasmid#154295PurposeFluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)DepositorInsert25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
ExpressionBacterialAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
C331
Plasmid#141123PurposeRhlI expressing plasmidDepositorAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only