32,168 results
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Plasmid#40865DepositorAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only
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pGBK CUP1 EGFP
Plasmid#170210PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFPDepositorArticleInsertenhanced green fluorescent protein
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-mTDGa.0
Plasmid#81050Purposebacterial expression of murine TDGDepositorAvailable SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-cyScarlet
Plasmid#191260PurposeBacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarletDepositorInsertcyScarlet
TagsHis tagExpressionBacterialAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCH6 - pBait-ChiX
Plasmid#174662Purposearabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.DepositorInsertChiX
Tags1xMS2 hairpinExpressionBacterialPromoterpBadAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWarf(+)
Plasmid#34562DepositorInsertHRV 3C-GPA-GFP-8HIS
ExpressionBacterialAvailable SinceFeb. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PEPD
Plasmid#31328DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12DKR-J
Plasmid#58306PurposeGeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.DepositorInsertGenomic cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_5+7_gfp
Plasmid#60595Purposeencodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.DepositorInsertCas_5+7_gfp
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_2+5
Plasmid#60594Purposeencodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2DepositorInsertCas_2+5
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTc302
Plasmid#207458PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertMTdTc302 (Dntt Mouse)
Tags10xHis-MBPExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg24 1B
Plasmid#197046PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.DepositorInsertAtg24
Tags6His-TEVExpressionBacterialAvailable SinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a HAB1 (172-511)
Plasmid#177844PurposeBacterial Expression of HAB1DepositorInsertProtein phosphatase 2C 16
Tags6xHis-GSTExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6c3
Plasmid#176211PurposeBacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6c3
Tags6XHisExpressionBacterialPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB230
Plasmid#170017PurposeEncodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Protegrin-1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
XNOR-gate-V2.0
Plasmid#44454DepositorInsertXNOR-gate
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
KTK_354
Plasmid#180525PurposeKTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
METT10D
Plasmid#25485PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMCSG53_Nsp15
Plasmid#167265PurposeBacterial expression of Nsp15DepositorInsertNsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
TagsHis6-TEV siteExpressionBacterialMutationnoneAvailable SinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-51b(+) ChemoG5
Plasmid#193805PurposeProtein production of ChemoG5 in bacteriaDepositorInsertChemoG5
Tags10xHisExpressionBacterialMutationEGFP:A206K, T225R; HT7: E143R, E147R, L271EPromoterT7Available SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Reb_140 aka PAS625
Plasmid#71230PurposeReb_1 with RebA(S88P)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA(S88P)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP -R
Plasmid#48887PurposePositive feedback plasmid from oscillator without luxRDepositorInsertsfGFP and luxI
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
130_pSKB3-LUMP-P.leioghnati
Plasmid#65894PurposeBacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetimeDepositorInsertLUMP
ExpressionBacterialAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #1
Plasmid#104208PurposeBacterial expression of WW domain #1 from NEDD4DepositorInsertNEDD4 WW domain #1 (NEDD4 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLitR-L1
Plasmid#74683PurposeLitR-L1 gateDepositorInsertPTac-RBSL1-LitR-PLitR-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
Tags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-Available SinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLMB51
Plasmid#40083DepositorInsertsgusA
tauR
tau promoter
ExpressionBacterialAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pETM30
Plasmid#85768Purposebacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
TagsGST and HisExpressionBacterialMutationcatalytic domain (aa 212-514)PromoterT7-lacAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR3-IR3-Bxb1-CadR-pZa
Plasmid#204686PurposeCadmium responsive genetic circuitDepositorInsertBxb1 (Bxb1p45 Synthetic)
UseSynthetic BiologyTagsCadRExpressionBacterialPromoterInducible, HspR is its repressorAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only