30,808 results

1. pLRD15

   Plasmid

   #208267

   Purpose

   Production of ent-sandaracopimaradiene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   ptmT2
   osKSL10
   

   Expression

   Bacterial

   Available Since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLBH532_MBP-Cas14b1 expression

   Plasmid

   #112505

   Purpose

   Expresses 10xHis-MBP fused to Cas14b1 under t7 promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b1
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET51b(+)_MBP-nLucCP-67/68

   Plasmid

   #113922

   Purpose

   Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   MBP-NanoLucCP-67/68
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-15b-hAR-663-919

   Plasmid

   #89083

   Purpose

   bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

   Insert

   hAR ligand binding domain (AR Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 663-919

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. RHOA

   Plasmid

   #73231

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RHOA (RHOA Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00071:Ras 7-180

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ALDH18A1

   Plasmid

   #38947

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ALDH18A1 (ALDH18A1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pJK407

   Plasmid

   #72421

   Purpose

   Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   lipase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   lacks Met1 and Thr2 in annotated (v1) sequence

   Promoter

   T7

   Available Since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPINVL

   Plasmid

   #26040

   Depositor

   Ray Owens

   Article

   Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

   Insert

   u-LacZ
   

   Tags

   u-phosphatase secretion leader

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-3z/602

   Plasmid

   #26657

   Depositor

   Jonathan Widom

   Article

   Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pN006

    Plasmid

    #188470

    Purpose

    Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    AsCas12a
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBadcrtEBI_13

    Plasmid

    #63811

    Purpose

    expresses crtEBI genes for neurosporene production

    Depositor

    Howard Salis

    Article

    Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

    Insert

    crtEBI operon
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    PBAD

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTYB2-eIF4B

    Plasmid

    #37226

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF3
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pU6T7

    Plasmid

    #71462

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. K84M kinase-dead Nuak1-3xflag

    Plasmid

    #97219

    Purpose

    Plasmid expressing kinase-dead mutant Nuak1

    Depositor

    Huda Zoghbi

    Article

    Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

    Insert

    Nuak1 (NUAK1 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Kinase-dead K84M

    Promoter

    T7

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-SpyCatcher002-MBP

    Plasmid

    #216295

    Purpose

    Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SpyCatcher002-MBP
    

    Tags

    His6 tag and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMesh1

    Plasmid

    #175594

    Purpose

    Lac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    Mesh1 (Mesh1 Fly)
    

    Expression

    Bacterial

    Mutation

    Codon optimized for translation in E.coli. AGGAGG…

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_NR2F2_Hormone-recep

    Plasmid

    #109826

    Purpose

    Protein expression and purification of NR2F2_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR2F2_Hormone-recep (NR2F2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL0-BvDODAα1

    Plasmid

    #162528

    Purpose

    Beta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    DODAα1
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET11b_MutT

    Plasmid

    #73155

    Purpose

    Expresses in E. coli, E coli MutT

    Depositor

    Maurice Bessman, Sandra Gabelli

    Article

    Bessman et al J Biol Chem. 1996 Oct 11;271(41):25059-6

    Insert

    MutT
    

    Expression

    Bacterial

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only