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Plasmid#103976PurposePlasmid encoding SapphireDepositorInsertSapphire
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S72A, Y145F,…PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA
Plasmid#99875PurposeExpression of GFP-AURKA for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEMG-RIS
Plasmid#225132PurposeFor genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
HisMS2_VLP_FluA
Plasmid#175955PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]DepositorInsertM gene
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pBS hTSP-3
Plasmid#12995DepositorInsertTSP-3 (THBS3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-mko1
Plasmid#78467PurposeBroad range bacterial fluorescence labelingDepositorInsertmKO1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mKOκ-EC18154
Plasmid#137077PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
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SpyTag003-OspC
Plasmid#159998PurposeExpresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cellsDepositorInsertSpyTag003-OspC (20-209) C129S
UseTagsHis6-tag and Thrombin cleavage siteExpressionBacterialMutationC129S mutation improves expression level.PromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His10-SHP1_PTPase (243-541aa)
Plasmid#180812PurposeExpression of 10*His tagged human SHP1 phosphatase domain (243-541aa)DepositorInsertSHP1_PTPase (243-541aa) (PTPN6 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAN3944
Plasmid#74702PurposeCircuit plasmid: 0x1CDepositorInsertCircuit 0x1C
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH385-GST-TEV-SUP35
Plasmid#29744DepositorInsertSUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
UseTagsGSTExpressionBacterialMutationwild typePromoterAvailable sinceAug. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2D4
Plasmid#15786DepositorInsertUbiquitin conjugating enzyme UbE2D4 (UBE2D4 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEW3: pET-32a Dot1L(2-416)
Plasmid#124098PurposeDot1L(2-416) expression plasmidDepositorInsertDot1L(2-416) (DOT1L Human)
UseTagsTrx-His-TEVExpressionBacterialMutationPromoterT7Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-3xHA-SAPAP3
Plasmid#190200PurposeExpresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytesDepositorInsert3xHA-SAPAP3 (Dlgap3 Mouse)
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
Plasmid#101230PurposeBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C, D273C, N692A, M694A, Q695A and…PromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS2k-RFP
Plasmid#35330DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTetAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAB078d9
Plasmid#79207PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1+2+3); Ppro1Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His6 ProteinG TEV LIC cloning vector (2P-T)
Plasmid#29713DepositorTypeEmpty backboneUseTagsHis6-ProteinG-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only