32,218 results
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Plasmid#8562DepositorInsertHOXA5 (HOXA5 Human)
TagsHis and T7ExpressionBacterialMutationSacI site at amino acids 1-2 was used to clone in…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-SUMO-sfGFP-WT
Plasmid#212122PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
TagsHis6 and SUMOExpressionBacterialPromoteraraC and lppAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-SUMO-TAG35-sfGFP
Plasmid#212120PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-TAG35-sfGFP
M. alvus Pyl-tRNA
TagsHis6 and SUMO-TAG35ExpressionBacterialMutationE35TAG (within SUMO encoding sequence)PromoteraraC and lppAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGBW-m4046758
Plasmid#145670PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
K560GFP_17NL
Plasmid#129767PurposeExpresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assaysDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP S2TAG/E17TAG
Plasmid#68298PurposeS2TAG/E17TAG GFP reporterDepositorInsertGFP S2TAG/E17TAG
TagsHisExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/mNeptune2-nat
Plasmid#120337PurposeBacterial mNeptune2 labelingDepositorInsertmNeptune2
ExpressionBacterialPromoterproDAvailable SinceAug. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mCFP-kanMX6
Plasmid#105145PurposeYeast gene targetingDepositorInsertmCFP-kanMX6
TagsmCFPExpressionBacterial and YeastMutationA206KAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
PEP119T
Plasmid#98251PurposePseudomonas T3SS gateway vector with HA-sfGFP11-sfGFP11DepositorInsertGateway cassettee-HA-sfGFP11-11
ExpressionBacterialAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB37
Plasmid#202602PurposeArabinose inducible expression vector expressing retron Rcat-Sen2DepositorInsertRcaT-Sen2 toxin
ExpressionBacterialAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFE-hCA
Plasmid#132999PurposepFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)DepositorAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Delta DED FLIP-L
Plasmid#13790DepositorInsertDELTA DED FLIP LONG (CFLAR Human)
Tags8 x His tagExpressionBacterialMutationDelta DED removes first 186 residuesAvailable SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
YSCD44A-c002
Plasmid#175493PurposeProtein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.DepositorInsertSingle chain Fv CD44 antibody (H90)
Tags3XFLAG-EK-His8 and pelBExpressionBacterialAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNSSCP
Plasmid#183188PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under TDepositorInsertsDivIVA and superfolder GFP fusion protein
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFAB815
Plasmid#47854PurposeBIOFAB reporter plasmid for measuring rnpB T1 termination efficiencyDepositorInsertrnpB T1 terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO9Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-HsPABPC1_J
Plasmid#146642PurposeBacterial Expression of HsPABPC1DepositorInsertHsPABPC1 (PABPC1 Human)
ExpressionBacterialMutationtwo silent mutations compared to the sequence giv…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
EPHB1_HUMAN_D0
Plasmid#79694PurposeThis plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
Plasmid#101230PurposeBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C, D273C, N692A, M694A, Q695A and…PromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKS014
Plasmid#65467PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidaseDepositorInsertNtag-beta galactosidase
UseSynthetic BiologyTagsFLAGExpressionBacterialPromoterpl-TetOAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRO16Sh2
Plasmid#180604PurposeExpression of ShCAST under control of Plac with pRO1600 origin.DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
UseSynthetic BiologyExpressionBacterialPromoterpLacAvailable SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSG81-pLas-tetO-deGFPssrA
Plasmid#102454PurposeExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.DepositorInsertdeGFP
TagsssrAExpressionBacterialPromoterpLas-tetOAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLT 652
Plasmid#59997PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRha-tns
Plasmid#170630PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
ExpressionBacterialAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL8B
Plasmid#67334PurposehArl8b with stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
PP-NMT
Plasmid#25200PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLmjF32.0080:P5-L421
TagsHisExpressionBacterialAvailable SinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
CaP2AIII_pK18
Plasmid#153421PurposeSource of murine TCR-alpha constant region (TRAC*02) gene followed by P2ADepositorInsertmurine TCR-alpha constant region (TRAC*02)-P2A
ExpressionBacterialAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
RAB2B
Plasmid#25534PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a(TIGIT)
Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only