30,234 results

1. pPBO.ACT003

   Plasmid

   #182713

   Purpose

   aTc inducible dCasRx-IF3

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   dCasRx
   

   Use

   CRISPR

   Tags

   3x(GGGS)-Escherichia Coli Initiation Factor 3

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pTet

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-His6-Ubc9-K14R

   Plasmid

   #185759

   Purpose

   Bacterial expression of His6-Ubc9-K14R

   Depositor

   Jeffrey Bode

   Article

   Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   K14R

   Promoter

   T7

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL1-mTFP1

   Plasmid

   #102635

   Purpose

   In vitro expression of mTFP1 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mTFP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CSNK1G1

   Plasmid

   #38934

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CSNK1G1 (CSNK1G1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. ETS2_pet28a

   Plasmid

   #131664

   Purpose

   Bacterial expression of ETS gene ETS2

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETS2 (ETS2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-mCherry-LOVSD

   Plasmid

   #81033

   Purpose

   Mammalian expression of the super-dark state of LOV2

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOVSD
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   C450A, L514K, G528A, L531E, N538E

   Promoter

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCoofy5

   Plasmid

   #43989

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His6-Sumo1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJKW0770

   Plasmid

   #119023

   Purpose

   E.coli expression plasmid for PmSPS1

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Insert

   PmSPS1
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKB230

   Plasmid

   #170017

   Purpose

   Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   Protegrin-1
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCol1a1-frt (FVB)

    Plasmid

    #63575

    Purpose

    Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.

    Depositor

    Ivo Huijbers

    Article

    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

    Insert

    Col1A-frt-hygro-pA
    

    Use

    Mouse Targeting; Frt/flpe

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    None

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGST-RXRalpha

    Plasmid

    #16547

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Insert

    RXR alpha (RXRA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-terminal DNA-binding domain: aa 1-224

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSUMO His6 SUMO PYL1 (36-211)

    Plasmid

    #177843

    Purpose

    Bacterial Expression of PYL1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    PYR1-like 1 (PYL1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEL1203

    Plasmid

    #196024

    Purpose

    Expression/purification of 6xHis-TEV-MlaC

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. R701-X22-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

    Plasmid

    #159571

    Purpose

    E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D (KRAS Human)
    

    Use

    Tags

    His6-MBP-tev-Avi

    Expression

    Bacterial

    Mutation

    G13D

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKCiR5

    Plasmid

    #149462

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CinR(AM)-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCin

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4Z3C (TET3)

    Plasmid

    #119969

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    TET3 (TET3 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 48-98

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pTL2-IncB-GFP11x7

    Plasmid

    #113031

    Purpose

    Expresses IncB tagged with 7 copies of GFP11 under a tetracycline inducible promoter

    Depositor

    Kevin Hybiske

    Article

    Wang et al Pathog Dis. 2018 Mar 1;76(2). pii: 48301

    Insert

    IncB (incB Chlamydia trachomatis)
    

    Use

    Tags

    GFP11x7 and flag

    Expression

    Bacterial

    Mutation

    Promoter

    tet inducible

    Available since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNB23

    Plasmid

    #103058

    Purpose

    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp

    Depositor

    Dirk Bumann

    Article

    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

    Insert

    GFP-OVA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hydrogen peroxide-inducible promoter katGp

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-6xHis-BglII

    Plasmid

    #196654

    Purpose

    Derived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII site

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRiboI-Sec-APEX2m

    Plasmid

    #176845

    Purpose

    Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

    Depositor

    Jessica Seeliger

    Article

    Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

    Insert

    pRibo-Sec-APEX2m
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRibo, theophilline riboswitch inducible hsp60 pr…

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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