30,234 results

1. pRSFDuet-1_VNp-hGH

   Plasmid

   #182411

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-hGH (GH1 Human)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB2-mRuby3

   Plasmid

   #104004

   Purpose

   Plasmid encoding mRuby3

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mRuby3
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGV3340

   Plasmid

   #170278

   Purpose

   Expression of mutant HIV-2 RT p51 reverse transcriptase

   Depositor

   Gregory Van Duyne

   Article

   Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

   Insert

   HIV-2 RT p51 (mutant)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pE4n

   Plasmid

   #17458

   Purpose

   modified pENTR vector for N-terminal GST tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   GST and Thrombin site

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGC_RT_mScarlet

   Plasmid

   #136967

   Purpose

   Goldengate/Greengate CDS module, containing mScarlet

   Depositor

   Klaus Theres

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINCDF

   Plasmid

   #41132

   Depositor

   Ray Owens

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn5-130

   Plasmid

   #118516

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHC01

   Plasmid

   #106900

   Purpose

   Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

   Depositor

   Jonathan Silberg

   Article

   Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

   Inserts

   LasR
   Methyl Halide Transferase
   Ethylene Forming Enzyme
   Red Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DNMT3b KO Neo

   Plasmid

   #16651

   Depositor

   Bert Vogelstein

   Article

   Rhee et al Nature. 2002 Apr 4. 416(6880):552-6.

   Insert

   DNMT3b knock-out construct (DNMT3B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pET15b/GgVcl 884-1066-ECFP

    Plasmid

    #46182

    Depositor

    Susan Craig

    Insert

    Vcl 884-1066 (VCL Chicken)
    

    Use

    Tags

    ECFP and His

    Expression

    Bacterial

    Mutation

    aa 884-1066 (tail domain)

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pDOC-GG

    Plasmid

    #149377

    Purpose

    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Inserts

    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLAX13 (CT#232)

    Plasmid

    #13918

    Depositor

    Cliff Tabin

    Article

    Logan et al Methods. 1998 Apr . 14(4):407-20.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pET21-pelB-LaG-41

    Plasmid

    #172757

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSM040

    Plasmid

    #128294

    Purpose

    asymmetric plasmid partitioning E. coli - target plasmid

    Depositor

    Matthew Bennett

    Article

    Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

    Insert

    Piq - mRFP, parS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-AtC60αβC20NtL

    Plasmid

    #160876

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: T7, chaperones: T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfuNca-Plastid

    Plasmid

    #190818

    Purpose

    Plastid targeting GFP with ble resistant gene for zeocin

    Depositor

    Peter Kroth

    Article

    Sprecher et al Algal Research. 2023 May; Vol 72: 103136

    Inserts

    Diatom plastid targeted GFP
    Bleomycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Cylindrotheca fusiformis fcp and Nitzschia captiv…

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1-A20 (OTU, aa 1-366)

    Plasmid

    #61584

    Purpose

    Expresses human A20 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Komander et al Biochem J. 2008 Jan 1;409(1):77-85.

    Insert

    A20 (TNFAIP3 Human)
    

    Use

    Tags

    GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 367-790.

    Promoter

    tac

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMP4639

    Plasmid

    #107771

    Purpose

    Expression of EYFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EYFP in pME6010
    

    Use

    Tags

    pLacZ-EYFP

    Expression

    Bacterial

    Mutation

    Promoter

    pLacZ

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#42

    Plasmid

    #153525

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#42

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#42
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZSm45 ndhII

    Plasmid

    #48889

    Purpose

    Lux-regulated expression of NDH-2 enzyme

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    ndhII and LuxR
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Mutation

    Promoter

    pLux

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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