30,234 results

1. pBAD-Ag43

   Plasmid

   #107743

   Purpose

   Expresses Ag43 under the control of an L-arabinose transcriptional regulator

   Depositor

   Ingmar Riedel-Kruse

   Article

   Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

   Insert

   Ag43 (flu )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   removed all pstI cut sites with silent mutations,…

   Promoter

   pBAD

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAT1A

   Plasmid

   #38852

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAT1A (Mat1a Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pDR111_Bgal NLS

   Plasmid

   #188396

   Purpose

   Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Use

   Tags

   PhoD secretion peptide and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Phyper spank

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21c(+)-mtPheRS

   Plasmid

   #31080

   Depositor

   Linda Spremulli

   Article

   Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

   Insert

   Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Promoter

   T7

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

5. phage UbiC SNAP-KDEL

   Plasmid

   #104982

   Purpose

   Lentiviral vector expressing SNAP-KDEL for ER labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   SNAP-KDEL
   

   Use

   Lentiviral

   Tags

   Secretion signal

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHCKAn-phoAp-GRFT

   Plasmid

   #158747

   Purpose

   low phosphate phoAp induction of Griffithsin

   Depositor

   Michael Lynch

   Article

   Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

   Insert

   phoAp Griffithsin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   phoA

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CMV-mCherry-hPMCA2x/b

   Plasmid

   #111571

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2x/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT29-splitD

   Plasmid

   #122599

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUMO-LIC-CHIKV-nsP2h

   Plasmid

   #127935

   Purpose

   Expresses CHIKV nsP2h (1-465 aa) in bacterial cells

   Depositor

   Dahai Luo

   Article

   Law et al Proc Natl Acad Sci U S A. 2019 May 7;116

   Insert

   CHIKV nsP2h
   

   Use

   Tags

   His6-Sumo-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule2-IBBN (G2)

    Plasmid

    #85501

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. RAB27B

    Plasmid

    #25545

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB27B (RAB27B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pET-3c-rhVTN-C

    Plasmid

    #30224

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

    Insert

    rhVitronectin with C terminus cleavage (VTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids: 399-478

    Promoter

    Available since

    May 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6NM4

    Plasmid

    #162257

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    PRDM9 (PRDM9 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. T7RNAP Cyslight V174C

    Plasmid

    #205078

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V174C

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7C-DNAJB6

    Plasmid

    #177657

    Purpose

    Recombinant protein synthesis of human DNAJB6

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    DNAJB6 (DNAJB6 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-T7Max-UTR1-deGFP-8xHis-T500

    Plasmid

    #178422

    Purpose

    Expression of deGFP via a T7 Promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    8xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pL0M-S-tdTomato-EC18158

    Plasmid

    #137078

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    tdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH6HTC_IGF2BP1-Halo

    Plasmid

    #175300

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-His10-SHP1_PTPase (243-541aa)

    Plasmid

    #180812

    Purpose

    Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SHP1_PTPase (243-541aa) (PTPN6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbB12a-GFP

    Plasmid

    #126115

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23151

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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