32,126 results
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WT A1-LCD +NLS
Plasmid#178658PurposeBacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)DepositorAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_T
Plasmid#74068PurposepAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecRDepositorInsertsgRNA pck (T)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterptacAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE141
Plasmid#118493Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ
Plasmid#191628PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZDepositorInsertlacZ
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLC-238
Plasmid#73192PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorAvailable SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS sfGFP
Plasmid#154467PurposepET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
B1-4
Plasmid#111466Purposetranslational elongation factor TuDepositorInserttranslational elongation factor Tu
Tags6xHisExpressionBacterialAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
DDX3XA
Plasmid#42362PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkHR113A
Plasmid#182292Purposeexpresses c-di-GMP mutant biosensor in E. coliDepositorInsertmVenusNB-MrkHR113A
TagsmVenusNB internal fusion to MrkH with a R113A poi…ExpressionBacterialMutationchanged arginine 113 to alanine, MrkH numbering …PromoterproCAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHN1_lacUV5-mNG-PDT
Plasmid#165583PurposeExpresses mNeonGreen and C-terminally fused PDTDepositorInsertmNeonGreen-PDT
TagsProtein degradation tagExpressionBacterialPromoterPtrcAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB078d8
Plasmid#79206PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2); Ppro1Available SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMMBGent
Plasmid#45475DepositorInsertGentamycin
ExpressionBacterialAvailable SinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-δWT-nAchR
Plasmid#97188PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertDelta nicotinic acetylcholine receptor (Chrnd Mouse)
ExpressionBacterialMutationWTPromoterT7Available SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP16/10
Plasmid#159615PurposeBacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10DepositorInsertsCovid-SARS2 Nsp16
Covid-SARS2 Nsp10
TagsHis6-Zb-TEV and noneExpressionBacterialMutationCodon-optimized for E. coli expressionPromoter(Bicistronic) and T7 - LacOAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA5-lenti
Plasmid#115664PurposeExpresses AcrVA5 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA5
UseLentiviralExpressionBacterial and MammalianPromoterEF-1a promoterAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIB190
Plasmid#90197PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneTagsHis-tag and Strep-tagExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension
Plasmid#176542PurposeBacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locusDepositorInsertsMBP tag
TnpB
Tags3' extension derived from endogenous locus a…ExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM112B
Plasmid#210602PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
TagsmClover3ExpressionBacterial and YeastPromoterSpH2A/Bpro (divergent)Available SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only
