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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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32,453 results

Showing: 8481 - 8500 of 32453 results

  9. RHOAA-c033

    Plasmid
    #205116
    Purpose
    RHOA protein expression
    Depositor
    Paul Brennan
    Insert
    RHOA (RHOA Human)
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205116-plasmid-map-sequence-id-411206

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  11. pMCSG53_Nsp9

    Plasmid
    #167264
    Purpose
    Bacterial expression of Nsp9
    Depositor
    Alexei Savchenko
    Insert
    Nsp9 (residues 1-113) (ORF1ab SARS-CoV-2)
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Available Since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167264-plasmid-map-sequence-id-331338

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  16. pETM33_Nsp3d_Ub2_PLPro

    Plasmid
    #156472
    Purpose
    Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp3d_Ub2_PLPro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156472-plasmid-map-sequence-id-307744

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  18. EHMT1 (2IGQ)

    Plasmid
    #25504
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EHMT1 (EHMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C-terminal domain, residues 951-1235
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. p404GAL1

    Plasmid
    #17417
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17417-plasmid-map-sequence-id-8592

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  20. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Tags
    4FLAG
    Expression
    Bacterial
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193315-plasmid-map-sequence-id-382367

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Showing: 8481 - 8500 of 32453 results