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pDG04439

Plasmid

#216365

Purpose

xhNup133-Nb2t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup133-Nb2t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGLO-Pink-SHE

Plasmid

#220781

Purpose

Expresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

Depositor

Angela Leu

Insert

raspberry chromoprotein (mRFP1)

Tags

6xHis

Expression

Bacterial

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-TEX264-deltaLRR-His6

Plasmid

#220215

Purpose

Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

Depositor

Kristijan Ramadan

Publication

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

His6

Expression

Bacterial

Mutation

c.4-75del / p.2-24del

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAGD248

Plasmid

#219498

Purpose

Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K

Expression

Bacterial

Promoter

pBAD

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pDmDERusk

Plasmid

#213702

Purpose

Vector for red-light-induced bacterial gene expression

Depositor

Andreas Moeglich

Publication

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Expression

Bacterial

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDrDERusk

Plasmid

#213703

Purpose

Vector for red-light-induced bacterial gene expression

Depositor

Andreas Moeglich

Publication

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Expression

Bacterial

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV099

Plasmid

#215305

Purpose

Expresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Publication

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PCymRC

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV094

Plasmid

#215302

Purpose

Expresses sfGFP under the Anderson J23119 promoter with a pRO1600 backbone.

Depositor

Brian Pfleger

Publication

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

J23119

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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peSCBE3-NG-Hypa

Plasmid

#218165

Purpose

This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

Depositor

Yuhui Sun

Publication

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

escbe3-NG-Hypa
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Expression

Bacterial

Mutation

Mutation in the portion of deaminase hAPOBEC3A : …

Promoter

rpsL promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT21.RB

Plasmid

#201007

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-mCherry-TagBFP, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
TagBFP

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11

Plasmid

#200991

Purpose

pLv1-pUC18R6KT-miniTn7-Gm, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pIV pm

Plasmid

#218101

Purpose

Phagemid that expresses LacI, and pIV under the Lac promoter

Depositor

Kevin Esvelt

Publication

Gretton et al bioRxiv 2024.03.20.585782

Insert

pIV

Expression

Bacterial

Promoter

Lac promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptet1

Plasmid

#218367

Purpose

Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptac

Plasmid

#218364

Purpose

Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1333

Plasmid

#217966

Purpose

Bacterial expression of RAN with an N-terminal 6His tag and a TEV cleavage site.

Depositor

Andrew Bowman

Publication

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

RAN (RAN Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_His-CBD-3C-EmPNGaseF

Plasmid

#218193

Purpose

Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

Depositor

Florence Pojer

Insert

PNGaseF

Tags

tufA-His-CBD-3C

Expression

Bacterial

Mutation

codon optimized Ecoli, residues 41-354

Promoter

T7

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NylCp2-TS_pET28a

Plasmid

#215421

Purpose

Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2-TS

Expression

Bacterial

Mutation

D122G/H130Y/D36A/E263Q

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LYS-Leu2

Plasmid

#218114

Purpose

Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

Depositor

Carl Denard

Publication

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-NH10-GG

Plasmid

#215806

Purpose

Cloning of target gene with N terminal non cleavable His10 tag

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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