30,235 results

1. MAC-C-GFP

   Plasmid

   #172973

   Purpose

   MAC-tagged GFP expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. H6SUMO-IntC-P53(304-393)

   Plasmid

   #200316

   Purpose

   bacterial expression of P53 TET-CTD for segmental labeling

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (304-393) (TP53 Human)
   

   Use

   Tags

   His6-SUMO and designed intein C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. H6GB1tevP53(1-303)IntN

   Plasmid

   #200312

   Purpose

   Bacterial expression of P53 NTAD-DBD for segmental labeling

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His6GB1tev and designed intein N

   Expression

   Bacterial

   Mutation

   M133L, V203A, N239Y, N268D

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-PAK2 wt

   Plasmid

   #31671

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   p21-activated protein kinase 2 (PAK2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pKSS

   Plasmid

   #39272

   Depositor

   Peter Kast

   Article

   Kast et al Gene. 1994 Jan 28;138(1-2):109-14.

   Type

   Empty backbone

   Use

   Positive-selection cloning vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. p109.1

   Plasmid

   #44518

   Depositor

   Michael Grunstein

   Article

   Hecht et al Cell. 1995 Feb 24;80(4):583-92.

   Insert

   Histone 4
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains full length (aa 1-102)

   Promoter

   Available since

   May 21, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCF1135

   Plasmid

   #222106

   Purpose

   E. coli - Veillonella shuttle plasmid with single selectable marker

   Depositor

   J Christopher Fenno

   Article

   Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

   Type

   Empty backbone

   Use

   E. coli-veillonella shuttle plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pET28a_DmKHC[1-421]-SNAP-6xHis

   Plasmid

   #196975

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   6xHis and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-GFP-mHP1alpha

   Plasmid

   #181912

   Purpose

   Bacterial protein expression vector for GFP-tagged HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM33_WDR5_WD

    Plasmid

    #178491

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    WDR5_WD (WDR5 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-parkin 141-465

    Plasmid

    #45971

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 141-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6C1A (MBD2)

    Plasmid

    #119958

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD2 (MBD2 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAKgfp2

    Plasmid

    #14077

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    lacIq repressor gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pSF-pMB1'-t7- YFP

    Plasmid

    #186564

    Purpose

    pMB1' ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-4T2 Parkin RBR

    Plasmid

    #31255

    Depositor

    Rachel Klevit

    Article

    Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

    Insert

    Parkin (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    RBR domain, residues 217-465

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pJKR-L-cdaR

    Plasmid

    #62558

    Purpose

    Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBbA0c-RFP

    Plasmid

    #35373

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBbS0k-RFP

    Plasmid

    #35369

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28b-MBP-TEV-PWWP_DNMT3A

    Plasmid

    #186970

    Purpose

    Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    DNMT3A PWWP domain (DNMT3A Human, Synthetic)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    PWWP domain (residues 278–427)

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAN-NOR

    Plasmid

    #62306

    Purpose

    synthetic circuit: NOR gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Inserts

    A2NT sgRNA
    A2NT sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF and pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge