30,237 results

1. BB3rN_14*

   Plasmid

   #98550

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SBC009876

   Plasmid

   #226283

   Purpose

   Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

   Depositor

   Nigel Scrutton

   Article

   Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

   Inserts

   Coniferyl alcohol acyltransferase
   Eugenol synthase 1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEAST_J23101-amidase

   Plasmid

   #128135

   Purpose

   The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

   Depositor

   Jean-Loup Faulon

   Article

   Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

   Insert

   amidase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. L1dRSLE

   Plasmid

   #13266

   Depositor

   Vance Lemmon

   Article

   Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   RSLE region removed

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pRSETb-tVYN-TmΔ

   Plasmid

   #149694

   Purpose

   High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

   Depositor

   Ming Hammond

   Article

   Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

   Insert

   VenusΔC12-TmYcgRΔ-NLucΔ4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PdnaK-IR3-IR3-Bxb1-CadR-pZa

   Plasmid

   #204686

   Purpose

   Cadmium responsive genetic circuit

   Depositor

   Urartu Seker

   Article

   Akboga et al Biosensors (Basel). 2022 Feb 16;12(2):12

   Insert

   Bxb1 (Bxb1p45 Synthetic)
   

   Use

   Synthetic Biology

   Tags

   CadR

   Expression

   Bacterial

   Mutation

   Promoter

   Inducible, HspR is its repressor

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSP1D1R1

   Plasmid

   #97323

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1R1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of R residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasyG1_hph

   Plasmid

   #184912

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSW003

   Plasmid

   #111249

   Purpose

   Broad host-range bacterial vector

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pC002 - LshC2C2 locus into pACYC184

    Plasmid

    #79151

    Purpose

    Endogenous LshC2c2 locus cloned into pACYC184

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    LshC2c2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. SLX4

    Plasmid

    #73242

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SLX4 (SLX4 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX SH2-B-SH2

    Plasmid

    #46479

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2-B (SH2B1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 515-639

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-6His-SUMO-Nsp14

    Plasmid

    #154956

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pTE103

    Plasmid

    #59398

    Purpose

    MethyloBrick expression vector, fumC promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PfumC

    Available since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UBC13-PGEX4T

    Plasmid

    #25464

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    Ubc13 (UBC13, MMS2 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pET22b-EC12WTC9A

    Plasmid

    #47924

    Purpose

    Expresses E-cadherin EC12 WT with C9A mutation in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Use

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    changed Cysteine 9 to Alanine

    Promoter

    T7

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103 BAG3 WW domain

    Plasmid

    #104266

    Purpose

    Bacterial expression of WW domain from BAG3

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    BAG3 WW domain (BAG3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-MRPP1

    Plasmid

    #67862

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP1 (TRMT10C Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. dFP-mini

    Plasmid

    #120543

    Purpose

    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

    Depositor

    Brian Chow

    Article

    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

    Insert

    de novo fluorescence-activating protein mini (dFP-mini)
    

    Use

    Tags

    6xHis - TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAL630

    Plasmid

    #167836

    Purpose

    Encodes OptoLAC3 circuit

    Depositor

    Jose Avalos

    Article

    Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

    Insert

    lacI
    

    Use

    Synthetic Biology

    Tags

    ssrA (AAV)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge