33,128 results
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Plasmid#196185PurposeCo-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-HisDepositorInsertsTagsGST, HA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only
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pXD70LacZ6-LacI-Ptac(LacO)-LacZ
Plasmid#191621PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2XDepositorInsertlacZ
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.0-Pct5.1-crRNA(AarI)
Plasmid#191633PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDrop
Plasmid#184872PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
csr-1_hH1_mEos3.1_V5
Plasmid#191750PurposePhotoconvertible fluorescent nuclear reporter.DepositorAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod2S-GG
Plasmid#191346PurposeClostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHN1_lacUV5-mNG-PDT
Plasmid#165583PurposeExpresses mNeonGreen and C-terminally fused PDTDepositorInsertmNeonGreen-PDT
TagsProtein degradation tagExpressionBacterialPromoterPtrcAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.436
Plasmid#184953PurposeExpress -Eco1 RT and ncRNADepositorInsertEco1: ncRNA(wt) and RT
ExpressionBacterialPromoterT7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PAJ698
Plasmid#178947PurposeExpresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)DepositorInsertChimeric Tail Fiber Fusion P2-gpH and S16 gp37
UseUnspecifiedExpressionBacterialAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6_rpoA-U
Plasmid#190967PurposeTest the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_nat
Plasmid#184911PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHG7
Plasmid#188255PurposeIntegrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB083
Plasmid#181952PurposeIPTG-inducible expression of PhoQDepositorInsertPhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
ExpressionBacterialPromoterPhoQ-Ptac; lacI-PlacIqAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-lepB
Plasmid#190725Purposefine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-lepB
ExpressionBacterialAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1009 (pSL9)
Plasmid#191004PurposeExpresses E2 Crimson on a broad host range pBBR1 origin plasmidDepositorInsertE2-Crimson
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1003 (pSL2)
Plasmid#190998PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-3C-FIP200-GFP
Plasmid#187832PurposePlasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-NDP52
Plasmid#187828PurposePlasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His
Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
TagsHA-6HIS and MKIEEExpressionBacterialAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only