30,258 results
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Plasmid#78202PurposeExpression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A D63C T174A T175N D224C C287A)
UseTagsHis6ExpressionBacterialMutationK33A D63C T174A T175N D224C C287APromoterunknownAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-E2
Plasmid#194590PurposeBacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertE2 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE
Plasmid#73438PurposeProdeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.DepositorInsertPG6-vioABE
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPG6 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-Optimized E6AP
Plasmid#165100Purposeecoli optimized expression of GST-fusion of human E6APDepositorInsertE6AP isoform 3 (UBE3A Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB1 (CK2alpha)
Plasmid#27083DepositorInsertCK2alpha (CSNK2A1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-ABK
Plasmid#126035PurposeEfficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.DepositorInsertstRNA synthetase 1
tRNA synthetase 2
tRNA for TAG codon
UseTagsExpressionBacterialMutationL274M, C313A, Y349FPromoteraraBAD promoter, glnS promoter, and proK promoterAvailable sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-33
Plasmid#214342PurposeProtein overexpression in bacteriaDepositorInsertIL33 (IL33 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
GST-RNF126
Plasmid#138643PurposeBacterial expression of GST-RNF126 fusion proteinDepositorInsertRNF126 (RNF126 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-eCFP
Plasmid#78476PurposeBroad range bacterial fluorescence labeling (ECFP)DepositorInserteCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52
Plasmid#98678PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-ultraID
Plasmid#172879Purposeexpresses ultraID in E. coliDepositorInsertultraID
UseTagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-Smad3 WT
Plasmid#27010DepositorInsertSmad3 (SMAD3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISTfu1_reRNA
Plasmid#205355PurposeBacterial expression of ISTfu1 TnpB and its reRNADepositorInsertISTfu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
p405MET25
Plasmid#15961DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-T4gp43(WT)
Plasmid#228203PurposeExpression in BL21(DE3) of WT T4 DNA polymeraseDepositorInsert43
UseTagsSce VMA intein-chitin binding domainExpressionBacterialMutationPromoterT7Available sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-Ubiquitin
Plasmid#223236PurposeBacterial expression construct for GST-Tagged Ubiquitin production and purificationDepositorInsertUbiquitin
UseTagsExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE80l ACA
Plasmid#196471PurposeProtein expression of SpyTag-ELP-CarHC-ELP-SpyTagDepositorInsertACA
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-C7
Plasmid#194591PurposeBacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertC7 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDawn-Ag43
Plasmid#107742PurposeExpresses Ag43 under the control of a blue-light transcriptional regulatorDepositorInsertAg43 (flu )
UseTagsExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpLambdaAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only