30,735 results

1. pCgCas9_recT

   Plasmid

   #153337

   Purpose

   Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pP2-phiV10

   Plasmid

   #196333

   Purpose

   Plasmid expressing chimeric P2-phiV10 tail fiber

   Depositor

   Baojun Wang

   Article

   Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

   Insert

   chimeric tail fiber P2-phiV10
   

   Expression

   Bacterial

   Promoter

   P2 late promoter

   Available Since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPSU150

   Plasmid

   #173836

   Purpose

   Expresses 150 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABMBPpepN
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbS5a-OptoCre-REDMAP

   Plasmid

   #228465

   Purpose

   Light-inducible Cre recombinase split with REDMAP photoreceptors for bacterial expression

   Depositor

   Mary Dunlop

   Article

   Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

   Insert

   OptoCre-REDMAP
   

   Use

   Cre/Lox

   Expression

   Bacterial

   Promoter

   pLacUV5

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-GST-His6-2_HBD

   Plasmid

   #171870

   Purpose

   Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

   Depositor

   Kaiwei Liang

   Article

   Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

   Insert

   Tandem repeats Hybrid binding domain of Rnase H1
   

   Tags

   GST- and His10-tag

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE-Tn5-145

   Plasmid

   #118529

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTcI42-Wasabi

   Plasmid

   #86118

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI42
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, K6N, S33T, Y61H, L119P, F122C

   Promoter

   pR/pL and pR/pL, LacI

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b(+)_rPD-L1nb

   Plasmid

   #226715

   Purpose

   Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Tags

   HA tag and Histidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLZ12Km2-P23R:TA:fflucRT

   Plasmid

   #88901

   Purpose

   Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

   Depositor

   Thomas Proft

   Article

   Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

   Insert

   toxin-antitoxin + Red-shifted firefly luciferase
   

   Expression

   Bacterial

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTcI-Wasabi

    Plasmid

    #86101

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    pR/pL and pR/pL, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-BiFC

    Plasmid

    #87856

    Purpose

    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    

    Tags

    BiFC C-terminal fragment

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pP2-P1(S')

    Plasmid

    #196334

    Purpose

    Plasmid expressing chimeric P2-P1(S') tail fiber

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    chimeric tail fiber P2-P1(S') and chaperone protein U'
    

    Expression

    Bacterial

    Promoter

    P2 late promoter

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGDP1 NDM-1

    Plasmid

    #112883

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    NDM-1 (blaNDM-1 Klebsiella pneumoniae)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C3-ZO-2

    Plasmid

    #27422

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pLVX-Tight-Puro_Olig2

    Plasmid

    #64842

    Purpose

    A lentiviral vector for the expression of mouse Olig2 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Olig2 (Olig2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. tFucci(SA)5

    Plasmid

    #153520

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(SA)5.
    

    Tags

    AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHyo182

    Plasmid

    #173136

    Purpose

    Expresses pheS_A294G in e.coli

    Depositor

    Seokhee Kim

    Article

    Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

    Insert

    pheS_A294G
    

    Expression

    Bacterial

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBB530

    Plasmid

    #27391

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    grpE
    

    Expression

    Bacterial

    Available Since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. YopH (residues 164-468)

    Plasmid

    #79749

    Purpose

    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    YopH (residues 164-468)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169193

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    14His-SUMO

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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