30,880 results

1. pAJM.717

   Plasmid

   #108517

   Purpose

   PTet*-YFP Reporter. aTc sensor for use in Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   PTet*-YFP
   

   Expression

   Bacterial

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPS0263

   Plasmid

   #133228

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPS0262

   Plasmid

   #133227

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29b-Ava3858-3856

   Plasmid

   #45745

   Depositor

   Emily Balskus, Christopher T Walsh

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   Ava3858-3856
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pETM6-FAS

   Plasmid

   #49810

   Purpose

   Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   fatty acyl-ACP thioesterase
   hydroxydecanoyl-ACP dehydratase
   b-ketoacyl-ACP synthase III
   3-ketoacyl-ACP reductase
   enoyl-(acyl carrier protein) reductase
   

   Expression

   Bacterial

   Mutation

   Codon-optmized. See supplemental information of …

   Promoter

   T7

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6CDG (GID4)

   Plasmid

   #119961

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   GID4 (C17orf39 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 124-289

   Promoter

   T7

   Available Since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. ALC1-c007

   Plasmid

   #173112

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   CHD1L (CHD1L Human)
   

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   L613-K879, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CuGFP-ATG8(416)

   Plasmid

   #49423

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

   Depositor

   Daniel Klionsky

   Article

   Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Promoter

   CUP1

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pENTR4-EGFP_opt

   Plasmid

   #106334

   Purpose

   Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Expression

   Bacterial and Mammalian

   Mutation

   codon optimized for gene synthesis without affect…

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQTEV-PSMD10

    Plasmid

    #31332

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. NSUN5

    Plasmid

    #25491

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NSUN5 (NSUN5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. SARS-CoV-2_N_deltalinker

    Plasmid

    #157870

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein without the linker

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    deltaliner

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTK164

    Plasmid

    #13533

    Depositor

    Wolf Frommer

    Article

    Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

    Insert

    FLIPW-CTY
    

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Available Since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGSTag Bmf

    Plasmid

    #24264

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    Bmf (BMF Human)
    

    Tags

    Gst

    Expression

    Bacterial

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pRU1097

    Plasmid

    #14462

    Depositor

    Philip Poole

    Article

    Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

    Insert

    gfp-mut3.1
    

    Expression

    Bacterial

    Available Since

    April 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. YWHAG

    Plasmid

    #39129

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    YWHAG (YWHAG Human)
    

    Tags

    Tev His6

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. p307HU

    Plasmid

    #27386

    Depositor

    David Bartel

    Article

    Shechner et al Science. 2009 Nov 27. 326(5957):1271-5.

    Insert

    unligated form of ligase ribozyme
    

    Expression

    Bacterial

    Available Since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. SIRT6

    Plasmid

    #41564

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    aa 3-318

    Promoter

    T7

    Available Since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pET28a-TGFα-GSY-SPM

    Plasmid

    #163615

    Purpose

    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    TGFa-GSY-SPM (TGFA Synthetic, Human)
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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