30,239 results

1. pET28-OmpT

   Plasmid

   #68862

   Purpose

   construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

   Depositor

   Neil Kelleher

   Article

   Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

   Insert

   OmpT (ompT )
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EHF_pet28a

   Plasmid

   #131660

   Purpose

   Bacterial expression of ETS gene EHF

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   EHF (EHF Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-4T1-WDR5

   Plasmid

   #59969

   Purpose

   Bacterial Expression GST-WDR5

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pBbB19a-GFP

   Plasmid

   #126131

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Human_TFAM_dHMGA_pET28

   Plasmid

   #167329

   Purpose

   Expresses TFAM dHMGA mutant in bacterial cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Truncation mutant containing linker, HMGB, and C-…

   Promoter

   T7

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. IBMc460

   Plasmid

   #161940

   Purpose

   Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PPhlF

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBbB15a-GFP

   Plasmid

   #126130

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23100

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBbB18a-GFP

   Plasmid

   #126133

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23117

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBbB20a-GFP

   Plasmid

   #126132

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   S23200

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBbB13a-GFP

    Plasmid

    #126116

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBbB10a-GFP

    Plasmid

    #126113

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23116

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MT2a

    Plasmid

    #105693

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 2a

    Depositor

    Artur Krężel

    Article

    Drozd et al Metallomics. 2018 Apr 25;10(4):595-613.

    Insert

    Metallothionein 2a (MT2A Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJOE6090.1

    Plasmid

    #135080

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

    Insert

    P-rhaP-BAD-Strep-tag II-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. gNS2B47NS3 L30SF31S

    Plasmid

    #155316

    Purpose

    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutant

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L30 to S, F31 to S

    Promoter

    M13

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-his6-HA3-HALO

    Plasmid

    #188943

    Purpose

    T7 expression vector for making his6- and HA3-tagged HALO protein

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    his6-HA3-HALO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDDRFP-A1B1

    Plasmid

    #36291

    Depositor

    Robert Campbell

    Article

    Alford et al Chem Biol. 2012 Mar 23;19(3):353-60.

    Insert

    Dimerization dependent RFP-A1B1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pHAGE2-TetOminiCMV-cMyc

    Plasmid

    #136614

    Purpose

    Dox-inducible lentiviral vector expressing mouse cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    cMyc (Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-28a-His6-SUMO-VAMP3

    Plasmid

    #135552

    Purpose

    Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP3
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

    Plasmid

    #222989

    Purpose

    Bacterial expression of iGeoCas9(G) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(G)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGex-6p2_FANCM

    Plasmid

    #221379

    Purpose

    Expresses FANCM in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Fanconi anemia group M protein (FANCM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge