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33,526 results

Showing: 8761 - 8780 of 33526 results

  2. pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

    Plasmid
    #168135
    Purpose
    Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.
    Depositor
    Feng Zhang
    Publication
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    Expression
    Bacterial
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168135-plasmid-map-sequence-id-330693

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  5. pLyGo-Ec-7

    Plasmid
    #163135
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Publication
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Nanobody, C-IgAP autotransporter
    Expression
    Bacterial
    Promoter
    P(rhaB)
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    163135-plasmid-map-sequence-id-332144

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  6. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Publication
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    169168-plasmid-map-sequence-id-332048

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  8. pMCSG53_Nsp15

    Plasmid
    #167265
    Purpose
    Bacterial expression of Nsp15
    Depositor
    Alexei Savchenko
    Insert
    Nsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Available since
    May 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167265-plasmid-map-sequence-id-331998

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  11. pID1072

    Plasmid
    #164869
    Purpose
    The pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.
    Depositor
    Keith Shearwin
    Publication
    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612
    Insert
    phiKO2 cB repressor
    Expression
    Bacterial
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    164869-plasmid-map-sequence-id-360033

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  16. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid
    #128404
    Purpose
    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Publication
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Exotoxin A (toxA P. aeruginosa)
    Tags
    6xHis tag and DsbA signal sequence for periplasmi…
    Expression
    Bacterial
    Mutation
    242DNNNS246 and 384DQNRT388 engineered glycosylat…
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128404-plasmid-map-sequence-id-261690

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  17. pJL1-MBP-4xDQNAT

    Plasmid
    #128390
    Purpose
    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites
    Depositor
    Michael Jewett
    Publication
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Maltose binding protein
    Tags
    4xDQNAT glycosylation tag and 6xHis tag
    Expression
    Bacterial
    Mutation
    A302T- please see depositor comment
    Promoter
    T7
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128390-plasmid-map-sequence-id-260183

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  20. pBBR1-Rha-ETh1h2e_yi23-kan

    Plasmid
    #166670
    Purpose
    Genome engineering of Burkholderia
    Depositor
    Jun Fu
    Insert
    ETh1h2e_yi23
    Expression
    Bacterial
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    166670-plasmid-map-sequence-id-329556

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Showing: 8761 - 8780 of 33526 results