30,245 results

1. pKSII/INSeGFP

   Plasmid

   #53787

   Purpose

   1 kb zebrafish insulin promoter driving eGFP

   Depositor

   Larry Moss

   Article

   diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.

   Insert

   INS promoter/eGFP/pA
   

   Use

   Zebrafish

   Tags

   eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   zebrafish insulin/ZIP

   Available since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pTRCHisB-TRF2∆M

   Plasmid

   #50489

   Purpose

   E. Coli N-term HexHis Tagged Protein Expression Vector

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF2 Delta Myb (TERF2 Human)
   

   Use

   Tags

   6x His, T7 tag, and TEV protease site

   Expression

   Bacterial

   Mutation

   Contains amino acids 3-437

   Promoter

   pTrc

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJMD13

   Plasmid

   #138653

   Purpose

   phiC31 integrating Streptomyces expression vector

   Depositor

   Joshua Blodgett

   Article

   Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PermE* variant sequence from pDA1652

   Available since

   June 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-2TK cyclin T1 (1-300) (P#432)

   Plasmid

   #14608

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

   Insert

   cyclin T1 (1-300) (CCNT1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-300

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

5. L1-4A

   Plasmid

   #13268

   Depositor

   Vance Lemmon

   Article

   Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pH6HTC_PEBP1-Halo

   Plasmid

   #175333

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PEBP1 (PEBP1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBRBB-Ppuf843-1200-PR

   Plasmid

   #50963

   Purpose

   Expresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroides

   Depositor

   Claudia Schmidt-dannert

   Article

   Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

   Insert

   Proteorhodopsin
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Ppuf843-1200

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK615

   Plasmid

   #71699

   Purpose

   Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSG84-pLas-TetRssrA

   Plasmid

   #102455

   Purpose

   Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   TetR (tetR )
   

   Use

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   pLas

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TBXTA-c006

    Plasmid

    #139755

    Purpose

    For protein expression/crystallography (PDB:6F59)

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, G177D (TBXT Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids E41-S224

    Promoter

    T7

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-HADH2

    Plasmid

    #67863

    Purpose

    bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    HADH2 (HSD17B10 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pE3c

    Plasmid

    #17463

    Purpose

    modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    6xMyc

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAN-PA4-RFP

    Plasmid

    #62250

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pA4

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046795

    Plasmid

    #145598

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pMCSG28-SH3-588

    Plasmid

    #185920

    Purpose

    Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

    Depositor

    Brian Kuhlman

    Article

    Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

    Insert

    SH3-588
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Designed enzyme with novel interface between huma…

    Promoter

    T7

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-AC3CtermGenomic-mCherry

    Plasmid

    #105250

    Purpose

    mouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    mCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. HTRA1_PDZ_1

    Plasmid

    #103912

    Purpose

    Protein expression and purification of HTRA1 PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    HTRA1 (HTRA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SIRT5H158Y-pET15b

    Plasmid

    #81032

    Purpose

    Bacterial expression of Human catalytic mutant SIRT5 protein

    Depositor

    David Lombard

    Article

    Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

    Insert

    Sirt5 (SIRT5 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    H158Y catalytic mutant

    Promoter

    T7

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-cyScarlet

    Plasmid

    #191260

    Purpose

    Bacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarlet

    Depositor

    Robert Campbell

    Insert

    cyScarlet
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJM160

    Plasmid

    #134169

    Purpose

    Bacterial expression of RB3 stathmin-like domain (SLD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    RB3-SLD (STMN4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues 49-189

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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