Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

Your Selections

Bacterial Expression

30,880 results

Showing: 8781 - 8800 of 30880 results

  2. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Expression
    Bacterial
    Promoter
    PBAD and PLtetO
    Available Since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

    Enlarge

  4. PTP1B-1-401

    Plasmid
    #78683
    Purpose
    bacterial expression of PTP1B aa 1-401
    Depositor
    Eli Chapman
    Insert
    PTPN1 (PTPN1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    delta 402-435
    Promoter
    araBAD
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78683-plasmid-map-sequence-id-152426

    Enlarge

  5. pET28-iLOV-Rsn2

    Plasmid
    #61687
    Purpose
    bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV
    Depositor
    Brian Smith
    Insert
    Ranaspumin-2
    Tags
    His and iLOV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61687-plasmid-map-sequence-id-259998

    Enlarge

  8. p35u4

    Plasmid
    #174786
    Purpose
    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system
    Depositor
    Katherine Berry
    Article
    Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.126
    Insert
    MS2 coat protein
    Tags
    λCI
    Expression
    Bacterial
    Mutation
    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…
    Promoter
    Synthetic Constitutive Promoter
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174786-plasmid-map-sequence-id-361842

    Enlarge

  12. NEK2

    Plasmid
    #39163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NEK2 (NEK2 Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    T175A inactivating mutant
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. RAB43

    Plasmid
    #25513
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB43 (RAB43 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pEF005-sgRNA-shuffle-in

    Plasmid
    #104441
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104441-plasmid-map-sequence-id-201145

    Enlarge

  19. pEF004-sgRNA-shuffle-in

    Plasmid
    #104440
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Promoter
    U6 promoter
    Available Since
    Dec. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    104440-plasmid-map-sequence-id-201143

    Enlarge

Showing: 8781 - 8800 of 30880 results