30,003 results

1. pTn7-SCOUT11.B

   Plasmid

   #201005

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-TagBFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   TagBFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL-p5X

   Plasmid

   #150814

   Purpose

   Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

   Depositor

   Paul Riggs

   Type

   Empty backbone

   Use

   Tags

   malE A313V

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 22, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDuet TDP43 WT

   Plasmid

   #27462

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Coexpression

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPROK AdoHcyase

   Plasmid

   #14683

   Depositor

   Michael Hershfield

   Article

   Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

   Insert

   adenosyl-homocysteine hydrolase (AHCY Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pConRef-2H12.D11

   Plasmid

   #221151

   Purpose

   cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2)

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. vGlut1 peptide- GST

   Plasmid

   #218677

   Purpose

   Expresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   vGlut (Slc17a6 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTn7-SCOUT21.RB

   Plasmid

   #201007

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-mCherry-TagBFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   mcherry
   TagBFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest-566

   Plasmid

   #11517

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. S48I48 ELP

   Plasmid

   #68394

   Purpose

   Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   S48I48 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ath5:GFP-CAAX

    Plasmid

    #105958

    Purpose

    transgenesis, EGFP membrane

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    CAAX
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBA2219

    Plasmid

    #38152

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

    Insert

    DsbC (dsbC E. coli)
    

    Use

    Tags

    N-terminal leader peptide (cloning artifact)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET24a-ncOGT-FL

    Plasmid

    #190821

    Purpose

    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E.coli

    Promoter

    T7

    Available since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pBWB294

    Plasmid

    #140636

    Purpose

    pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TRE3G-MYCN

    Plasmid

    #104542

    Purpose

    PiggyBac vector encoding doxycycline-inducible human MYCN

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCold TDP43 Q331K

    Plasmid

    #27454

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pTD103luxI_sfGFP

    Plasmid

    #48885

    Purpose

    Positive feedback plasmid from oscillator

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    LuxI, sfGFP, and LuxR
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Mutation

    Promoter

    pLux

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTOX6

    Plasmid

    #127451

    Purpose

    Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Inserts

    tse2 toxin
    amilCP chromoprotein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized from protein sequence in FPbase

    Promoter

    pRha and tac

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9(D10A)

    Plasmid

    #74495

    Purpose

    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    Cas9 D10A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    pTeto

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDae079 (eMutaT7[transition])

    Plasmid

    #187622

    Purpose

    Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Inserts

    TadA-8e-T7RNAP
    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ABE8e-Cas9NG

    Plasmid

    #203328

    Purpose

    Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    ABE8e-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pB006

    Plasmid

    #182320

    Purpose

    It's an empty replication plasmid backbone in Bacteroides

    Depositor

    Lei Dai

    Article

    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHLUK

    Plasmid

    #64181

    Purpose

    harbours marker genes HIS3, LEU2, URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pWT019a

    Plasmid

    #107893

    Purpose

    W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    PTetO-sd2U-Cas9, PLacO-sgRNA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSGKP-spe

    Plasmid

    #117234

    Purpose

    A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Pet22b-YAP

    Plasmid

    #166443

    Purpose

    Bacterial expression of 6x His tagged YAP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-53-RtcB

    Plasmid

    #51282

    Purpose

    E. coli expression vector for E. coli RtcB RNA ligase

    Depositor

    Jay Hesselberth

    Article

    Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

    Insert

    E. coli RtcB
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polymerase

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. T4 Lysozyme WT*

    Plasmid

    #18111

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No cysteines: C54T, C97A

    Promoter

    Available since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUC57-mitoTPNOX

    Plasmid

    #87854

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    mitoTPNOX
    

    Use

    Tags

    C-terminal FLAG tag with a linker and mitochondri…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMD19-PBBa_J23117-sgRNA-Spr

    Plasmid

    #190793

    Purpose

    Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA (dummy)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23117

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTn7-SCOUT11.Y

    Plasmid

    #201004

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-sYFP2, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    sYFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23100

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge