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pYTK035

Plasmid

#65142

Purpose

Encodes I-SceI (ORF) as a Type 3 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

I-SceI (ORF)

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK027

Plasmid

#65134

Purpose

Encodes pREV1 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pREV1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK009

Plasmid

#65116

Purpose

Encodes pTDH3 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pTDH3

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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PAS528 (TJF059)

Plasmid

#65602

Purpose

pETDuet-1 Containing FadD-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Promoter

T7 lacO

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pZS*11-yfp0

Plasmid

#53241

Purpose

express yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)

Depositor

Philippe Cluzel

Article

Subramaniam et al Proc Natl Acad Sci U S A. 2013 Feb 5;110

Insert

YFP

Expression

Bacterial

Promoter

PLtetO1

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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HGXPRT

Plasmid

#64201

Purpose

Expression of Plasmodium falciparum HGXPRT

Depositor

Vern Schramm

Article

Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi

Insert

Hypoxanthine guanine xanthine phosphoribosyltransferase

Tags

Histidine tag

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pSG 3/4

Plasmid

#63868

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UGA near N-term

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 853

Plasmid

#63873

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAA near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG lacZ

Plasmid

#63874

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

+1 frameshift near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 12DP

Plasmid

#63876

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

-1 frameshift near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 163

Plasmid

#63871

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 34-11

Plasmid

#63869

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UGA near N-term

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pJW4.16

Plasmid

#62871

Purpose

Bacterial expression of MBP-MBK-2

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2

Tags

MBP

Expression

Bacterial

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-AND

Plasmid

#62308

Purpose

synthetic circuit: AND gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A1NT sgRNA
A4NT sgRNA
A2NT sgRNA
A1NT

Use

CRISPR

Expression

Bacterial

Promoter

PA2, PPhlF, pA4, and pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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GST-auxilin(547-910)

Plasmid

#62572

Purpose

Codes for a GST-auxilin fusion protein that is functional as a co-factor in hsc70-dependent clathrin coat disruption

Depositor

Ernst Ungewickell

Article

Holstein et al J Cell Biol. 1996 Nov;135(4):925-37.

Insert

Auxilin

Tags

GST

Expression

Bacterial

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only
H6-epsin 1 (144-575)

Plasmid

#63008

Purpose

expresses the adaptor protein epsin that lacks its ENTH-domain in bacteria

Depositor

Ernst Ungewickell

Article

Kalthoff et al J Biol Chem. 2002 Mar 8;277(10):8209-16.

Insert

Epsin 1 without ENTH domain (Epn1 Rat)

Tags

His tag

Expression

Bacterial

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD2 (OTU, aa 132-314)

Plasmid

#61410

Purpose

Expresses human OTUD2 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-131 and aa 315-348.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_11

Plasmid

#60571

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_11

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_10

Plasmid

#60570

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_10

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge