30,880 results

1. dFP-mini

   Plasmid

   #120543

   Purpose

   Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

   Depositor

   Brian Chow

   Article

   Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

   Insert

   de novo fluorescence-activating protein mini (dFP-mini)
   

   Tags

   6xHis - TEV cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP18-merR_O

   Plasmid

   #127023

   Purpose

   Basic Part O- ORF; merR; transcription factor

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part merR_o
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC18T-mini-Tn7T-Gm-Pc-mKate2

   Plasmid

   #199249

   Purpose

   Fluorescent labelling of bacterial cells with mKate2

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized mKate2 gene
   

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKNR5

   Plasmid

   #149466

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   NahR(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PSalTTC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWJB30

   Plasmid

   #126505

   Purpose

   Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

   Depositor

   Ute Romling

   Article

   Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

   Insert

   adrA-8xHis
   

   Tags

   8xHis

   Expression

   Bacterial

   Promoter

   PBAD30

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-SK-QS

   Plasmid

   #24370

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   QS
   

   Expression

   Bacterial

   Available Since

   April 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTetQCas-8+IS186

   Plasmid

   #170636

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
   

   Expression

   Bacterial

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRE57I-GNAc

   Plasmid

   #170637

   Purpose

   DNA donor for transposition

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   Mini-Tn6677, GNAc cassette, lacI
   

   Expression

   Bacterial

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAH-CTX1-rhadCas9-native

   Plasmid

   #129392

   Purpose

   Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   S. pyogenes dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine, Histidine 840 to Alanine

   Promoter

   PrhaBAD

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1delZn3

    Plasmid

    #173936

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6MIW

    Plasmid

    #127291

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    6xHis-TEV (MHHHHHHSSGRENLYFQG)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pZ001 (AacC2c1 locus)

    Plasmid

    #70166

    Purpose

    Expresses AacC2c1 full locus

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    AacC2c1 locus
    

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b-T5-mad32TAG

    Plasmid

    #188997

    Purpose

    Express madanin-1 with TAG at 32 position

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Insert

    madanin-1 with TAG at 32 position
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b-T5-mad

    Plasmid

    #188998

    Purpose

    Express wildtype madanin-1

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Insert

    wildtype madanin-1
    

    Expression

    Bacterial

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEDF-PhdRS

    Plasmid

    #127445

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

    Depositor

    Wenshe Liu

    Article

    Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Expression

    Bacterial

    Mutation

    N346A-C348A mutations on PylRS

    Promoter

    araBAD

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-HA-MEK5

    Plasmid

    #53166

    Purpose

    Expresses GST-HA tagged human MEK5 from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. A0A075HNX4

    Plasmid

    #177695

    Purpose

    expresses a fatty alcohol oxidase in E. coli

    Depositor

    Martin Engqvist

    Article

    Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

    Insert

    Long chain alcohol oxidase
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJOE6090.1

    Plasmid

    #135080

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

    Insert

    P-rhaP-BAD-Strep-tag II-eGFP
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQadd2

    Plasmid

    #135662

    Purpose

    Clostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.

    Depositor

    Andrew Tolonen

    Article

    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

    Insert

    Lox511/66 and LoxFas/71
    

    Use

    Clostridial vector, pamβ1 origin

    Expression

    Bacterial

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTU1-A-SP44-mVenus-I

    Plasmid

    #163763

    Purpose

    Expresses Streptomyces codon-optimised mVenus-I

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    mVenus-I
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    SP44

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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