30,249 results

1. araC-pBAD-gusA-pBAV1k

   Plasmid

   #30502

   Depositor

   Ichiro Matsumura

   Article

   Murin et al Appl Environ Microbiol. 2011 Oct 21.

   Insert

   beta-glucuronidase (gusA Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHlyIIR-H1

   Plasmid

   #74681

   Purpose

   HlyIIR-H1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSH1-HlyIIR-PHlyIIR-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 130_pSKB3-LUMP-P.leioghnati

   Plasmid

   #65894

   Purpose

   Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetime

   Depositor

   Gerard Marriott

   Article

   Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

   Insert

   LUMP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-4T-2 RanGAP

   Plasmid

   #13379

   Depositor

   Mary Dasso

   Article

   Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

   Insert

   RanGAP (RANGAP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pH

   Plasmid

   #64178

   Purpose

   harbours marker gene HIS3

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET3d::strep-AID

   Plasmid

   #15924

   Depositor

   Nina Papavasiliou

   Article

   Dickerson et al J Exp Med. 2003 May 19. 197(10):1291-6.

   Insert

   AID (AICDA Human)
   

   Use

   Tags

   strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQTEV-STX6

   Plasmid

   #31581

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   syntaxin 6 (STX6 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHis-zfCSF3a

   Plasmid

   #65612

   Purpose

   express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

   Insert

   Csf3a (csf3a Zebrafish)
   

   Use

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pMS8: pTns(AvCAST)

   Plasmid

   #168141

   Purpose

   Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   AvTnsD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJ601

    Plasmid

    #29509

    Depositor

    Jonathan Tait

    Insert

    annexin VI (ANXA6 Human)
    

    Use

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1.

    Promoter

    Available since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pSEVA12DKR-J

    Plasmid

    #58306

    Purpose

    GeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Insert

    Genomic cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21d-colEwt2/Imwt2

    Plasmid

    #107127

    Purpose

    plasmid for expression of WT colicin endonuclease and immunity pair 2

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEwt2/Imwt2
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-MK2

    Plasmid

    #30187

    Depositor

    Bruce Tempel

    Insert

    MK2 (Kcna2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pTriEx-mVenus-Zdk1-Rac1 Q61L

    Plasmid

    #81137

    Purpose

    mammalian expression of Zdk1 tagged Rac1 Q61L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    Rac1 (RHOA Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. T7RNAP Cyslight A188C

    Plasmid

    #205083

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Insert

    T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S N88C

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T-1 EGFP-Sumo2

    Plasmid

    #13385

    Depositor

    Mary Dasso

    Article

    Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

    Insert

    Sumo2 (SUMO2 Human)
    

    Use

    Tags

    EGFP and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pGEX6p-1-PafA-XIAP

    Plasmid

    #113402

    Purpose

    Expresses PafA-XIAP in bacteria

    Depositor

    Min Zhuang

    Article

    Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

    Insert

    PafA-XIAP
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLITMUS*-PrpoN-CroAct3-PBBa_J23106-geneIII

    Plasmid

    #134354

    Purpose

    Phagemid

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    CroAct3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid

    #86668

    Purpose

    for generation of BirA tagged strains using CRISPR tagging technology

    Depositor

    David Sibley

    Insert

    Linker-BirA-3xHA-HXGPRT 3'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge