30,880 results
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pTn7xKS-dTomato
Plasmid#117395PurposeTn7 tagging vector pTn7xKS with dTomato expression scaffoldDepositorInsertdTomato
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-TelgRNA_PuroR
Plasmid#84782PurposeExpresses telomere-specific sgRNA.DepositorInserttelomere-specific sgRNA
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092694
Plasmid#153907PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stopDepositorInsertfragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationwtPromoterPromoter | t7 consensus from NEB, G is +1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXB1-EXO1b D173A
Plasmid#68269PurposeBacterial expression of human EXO1 D173A (catalytically-dead) mutantDepositorInsertEXO1 (EXO1 Human)
TagsMxe intein/chitin binding domainExpressionBacterialMutationcatalytically-dead D173A. Please see depositor co…PromoterT7Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA15_G-alpha
Plasmid#109782PurposeProtein expression and purification of GNA15_G-alphaDepositorInsertGNA15_G-alpha (GNA15 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-psba1-TetR-PL22-dCas9-SpR
Plasmid#73223PurposeContains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorInsertdCas9 from S. pyogenes
Tagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPL22Available SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCri_L830-antiSH2-OptoMB-V416L
Plasmid#159936PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
ExpressionBacterialMutationAsLOV2 V416LAvailable SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCri_L889-antiSH2-OptoMB-Triple
Plasmid#159937PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
ExpressionBacterialMutationAsLOV2 V416I_G528A_N538EAvailable SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHL 1075
Plasmid#53004PurposepCon:fhlA::mCherry, pLlacO-1:sodB::gfpDepositorInsertsfhlA
sodB
UseSynthetic BiologyTagsGFP and mCherryExpressionBacterialMutationcontains −309 to +96 bp relative to start codon a…PromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #3
Plasmid#104210PurposeBacterial expression of WW domain #3 from NEDD4DepositorInsertNEDD4 WW domain #3 (NEDD4 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMrfp
Plasmid#59773PurposeDerived from pCM62; ampR, tetR, PBAD - rfpDepositorInsertrfp
ExpressionBacterialPromoterpBADAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D
Plasmid#159541PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12DAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbB11a-GFP
Plasmid#126114PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23150Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-HALO
Plasmid#188943PurposeT7 expression vector for making his6- and HA3-tagged HALO proteinDepositorInserthis6-HA3-HALO
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-141
Plasmid#118525PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM33_TNKS_TANK-peptide-bind
Plasmid#178489PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertTNKS_TANK-peptide-bind (TNKS Human, Synthetic)
TagsHis-GSTExpressionBacterialPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBTBXh-1
Plasmid#26077DepositorTypeEmpty backboneTagsHisExpressionBacterialAvailable SinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only
