30,808 results

1. pILxR5

   Plasmid

   #149497

   Purpose

   Broad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLuxB

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Reb_65 aka PAS626

   Plasmid

   #71231

   Purpose

   Reb_1 with RebA(Y82A)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebA(G53A, Y82A)

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pJPK13

   Plasmid

   #80775

   Purpose

   Cloning vector compatible with ColE1 plasmids with high copy number

   Depositor

   Gregory Phillips

   Article

   Peterson et al Plasmid. 2008 May;59(3):193-201. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcrRNA.con

   Plasmid

   #61285

   Purpose

   Constitutive repeat-only E. coli CRISPR array

   Depositor

   Chase Beisel

   Article

   Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pG6(5'Pro) (P#1477)

   Plasmid

   #14659

   Depositor

   Matija Peterlin

   Article

   Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

   Insert

   UAS repeats
   

   Expression

   Bacterial

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pGMCKq1-10M1-sspBmyc

   Plasmid

   #27069

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

   Insert

   tetR-PmyctetO-sspB
   

   Expression

   Bacterial

   Available Since

   June 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSG020

   Plasmid

   #126503

   Purpose

   Pseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHis

   Depositor

   Ute Romling

   Article

   Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

   Insert

   disaggregase ClpGgi with 6xHis-tag
   

   Tags

   C-terminal 6xHis

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-45b-HOXD13-IDR-mCherry

   Plasmid

   #145280

   Purpose

   Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13-IDR (HOXD13 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVHD

   Plasmid

   #61303

   Purpose

   Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

   Type

   Empty backbone

   Tags

   HA

   Expression

   Bacterial

   Promoter

   PV10

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28b_NOSER

    Plasmid

    #124244

    Purpose

    Bacterial 6xHIS tagged protein expression without the addition of any potential phosphorylation sites to the tagged protein.

    Depositor

    Attila Fehér

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF525-EF1a-Hygro-P2A-AcrVA4-lenti

    Plasmid

    #115663

    Purpose

    Expresses AcrVA4 in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA4
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EF-1a promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGSTag Flag Jnk2a2

    Plasmid

    #15744

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a2 (MAPK9 Human)
    

    Expression

    Bacterial

    Available Since

    March 6, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pB2H2*

    Plasmid

    #163834

    Purpose

    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)

    Depositor

    Jerome Fox

    Insert

    SpecR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    TCPTP R222M

    Promoter

    Pro1;ProD;pLacZ-Opt

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIntegrase_10

    Plasmid

    #60581

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_10
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJS124

    Plasmid

    #207593

    Purpose

    GFP-BIB H20A expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-BIB H20A
    

    Expression

    Bacterial

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDWJ27

    Plasmid

    #207604

    Purpose

    GFP-BIB expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-BIB
    

    Expression

    Bacterial

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)

    Plasmid

    #86933

    Purpose

    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. RIT1

    Plasmid

    #55662

    Purpose

    expression of mouse RIT1

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RIT1 (Rit1 Mouse)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-NKX3.1

    Plasmid

    #42896

    Depositor

    Edward Gelmann

    Article

    Ju et al Biochemistry. 2009 Nov 10;48(44):10601-7

    Insert

    NKX3.1 (NKX3-1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

20. MK14_HUMAN_D0

    Plasmid

    #79680

    Purpose

    This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK14
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only