32,686 results
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Plasmid#176232PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4out
Plasmid#176233PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30del
Plasmid#106902PurposepQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-141
Plasmid#118525PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWJB30
Plasmid#126505PurposeSalmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30DepositorInsertadrA-8xHis
Tags8xHisExpressionBacterialPromoterPBAD30Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBCiG2
Plasmid#149470PurposeBroad-Host range expression vector, pBBR origin, CinR(AM) activator, PCin promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertCinR(AM)-GFP
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
TagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM555
Plasmid#68880PurposeNanoLuc expressed constiutively from PcfxA, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLacI241-Wasabi
Plasmid#86102PurposemWasabi reporter gene gated by temperature-sensitive LacI A241TDepositorInsertsLacIts
mWasabi
Tags6xHisExpressionBacterialMutationA241TPromoterT7A1O4/O3 and pLacIAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTE-mcs
Plasmid#20320DepositorInsertmultiple cloning site
ExpressionBacterialAvailable SinceMarch 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-Myc
Plasmid#119752PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.DepositorTypeEmpty backboneTagsGST and MycExpressionBacterialPromotertac promoterAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3(1-265)
Plasmid#100573PurposeExpresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.DepositorInsertStx3-1-265 (Stx3 Rat)
TagsGSTExpressionBacterialMutationNo transmembrane domain, residues 4-264 present.PromotertacAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN11
Plasmid#38965PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
Tags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
monomer-NI
Plasmid#27966DepositorInsertTAL-monomer-NI
ExpressionBacterialAvailable SinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
monomer-NG
Plasmid#27967DepositorInsertTAL-monomer-NG
ExpressionBacterialAvailable SinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
TRBV12-3
Plasmid#123445Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRBV7-6
Plasmid#123434Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRBV30
Plasmid#123460Purposeconstruction of TCRDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only