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pLG169

Plasmid

#124367

Purpose

Xylose inducible system with the CRE site for expression in B. subtilis

Depositor

Christopher Voigt

Publication

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pxyl

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMCSG7(gfcC)

Plasmid

#176197

Purpose

gfcC protein expression

Depositor

Mark Saper

Publication

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcC

Tags

6xHis-TeV

Expression

Bacterial

Promoter

T7

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_mmHUR-Halo

Plasmid

#175311

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (Elavl1 Mouse)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-HBB-nLuc

Plasmid

#175432

Purpose

Used to generate IVT RNA for translation extracts

Depositor

Britt Glaunsinger

Publication

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

HBB-nLuc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172504

Purpose

Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF RBP-FL P53

Plasmid

#174042

Purpose

Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

Depositor

Stewart Loh

Publication

Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

Insert

P53 gene (TP53 Human)

Tags

Thermoanaerobacter Tencongenesis ribose binding p…

Expression

Bacterial

Promoter

T7

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCK255

Plasmid

#171155

Purpose

Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Publication

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

I-SceI_sacB

Expression

Bacterial

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

Plasmid

#174424

Purpose

Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

Depositor

Thomas Pucadyil

Publication

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1 (DNM1L Human)

Tags

6xHis(Thrombin)EGFP(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pETx-SR6c3

Plasmid

#176211

Purpose

Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Publication

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6c3

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Publication

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Expression

Bacterial and Insect

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i3-TEV-Strep

Plasmid

#174501

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i3

Depositor

Manos Mavrakis

Publication

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)

Tags

TEV-Strep

Expression

Bacterial

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD_Ub11TCA

Plasmid

#174517

Purpose

Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

Depositor

Jason W Chin

Publication

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

Ubiquitin

Tags

6xHis

Expression

Bacterial

Mutation

Recoded with TCA replacing codon 11 of wt ubiquit…

Promoter

araBAD

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS1

Plasmid

#145342

Purpose

Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term eGFP

Depositor

Rob Parton

Publication

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDTFendoNA2

Plasmid

#174731

Purpose

Expresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.

Depositor

Jukka Finne

Publication

Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

Insert

DT386F

Expression

Bacterial

Mutation

Deleted amino acids 412-560 (the receptor binding…

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH625_pJH_LldR_PlldR-sfGFP.0TAG

Plasmid

#163724

Purpose

Lactate-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp

Expression

Bacterial

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH474_pTech_Para-supP

Plasmid

#163718

Purpose

Arabinose-inducible expression of supP

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

supP

Expression

Bacterial

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

Plasmid

#163719

Purpose

Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG

Expression

Bacterial

Mutation

dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH643

Plasmid

#172168

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam

Expression

Bacterial

Mutation

sfGFP(N39*, N135*, Y151*)

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH645

Plasmid

#172169

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

Inserted amber codon immediately after initiator …

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINO_F2

Plasmid

#171926

Purpose

Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Publication

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_F2

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Available since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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