30,808 results

1. MetNI (Lm)

   Plasmid

   #78316

   Purpose

   Methionine ABC Transporter

   Depositor

   Douglas Rees

   Article

   Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

   Inserts

   MetI
   MetN
   

   Tags

   10x Histidine Tag and 6x Histidine Tag

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCSG7-H588A-NmeCas9

   Plasmid

   #71477

   Purpose

   bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-H588A-NmeCas9
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   H588A

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SARS-CoV-2_N_deltaNIDR

   Plasmid

   #157868

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDR

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   deltaNIDR

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGST-HsSyx-375-792

   Plasmid

   #179292

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Tags

   His6 + GST + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   residues 375-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET32a-Spike-S-4P

   Plasmid

   #165094

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
   

   Expression

   Bacterial

   Available Since

   March 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Dhh1-EGFP

   Plasmid

   #201418

   Purpose

   Bacterial protein expression of Dhh1

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Dhh1 (DHH1 Budding Yeast)
   

   Tags

   EGFP, TEV, 6xHis and MBP, TEV

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. tol2-mpx-cxcr4b whim-gfp

   Plasmid

   #41089

   Depositor

   Anna Huttenlocher

   Article

   Walters et al Blood. 2010 Oct 14;116(15):2803-11. Epub

   Insert

   CXCR4b-WHIM (cxcr4b Zebrafish)
   

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   deleted last 19 amino acids of cxcr4b

   Promoter

   mpx

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPSB2

   Plasmid

   #38983

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SPSB2 (SPSB2 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pET-deSpCas9-VP64-6xHis

   Plasmid

   #92117

   Purpose

   Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB164

    Plasmid

    #101667

    Purpose

    Opto-T7RNAP*(563), half expr.

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563), half expr.
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-HCF-1rep1E10A

    Plasmid

    #84607

    Purpose

    Plasmid contains HCF-1rep1E10A with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1E10A (HCFC1 Human)
    

    Tags

    GST and His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-HCF-1rep1wt

    Plasmid

    #84606

    Purpose

    Plasmid contains HCF-1rep1wt with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1wt (HCFC1 Human)
    

    Tags

    GST-TEV and His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-M-3A2

    Plasmid

    #73435

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 3A2 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

    Plasmid

    #83484

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1048A, H1053A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-PRDM16 880-1038

    Plasmid

    #53350

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 880-1038

    Promoter

    tac

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. GST-PRDM16 224-454

    Plasmid

    #53347

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 224-454

    Promoter

    tac

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pBAD*RFPEC2

    Plasmid

    #17823

    Depositor

    Jay Keasling

    Article

    Pfleger et al Biotechnol Bioeng. 2005 Dec 5. 92(5):553

    Insert

    E. coli optimized red flourescent protein (RFPec2)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. hisSUMO-ZFP57

    Plasmid

    #102716

    Purpose

    Express DNA binding domain of human Zinc finger protein 57 in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    Zinc finger protein 57 (ZNF57 Human)
    

    Tags

    hisSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPSP6+t

    Plasmid

    #48149

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCoofy12

    Plasmid

    #43977

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    OneStrep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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