33,128 results
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Plasmid#160433PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to ThrDepositorInsertE. coli AlkB
TagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Threonine; deleted t…PromoterT7Available SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTHMT_hnRNPF_FL
Plasmid#139114Purposeexpress FL hnRNPF with N-terminal maltose binding protein tagDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtTcR1AtR2NtB2
Plasmid#160889PurposeExpression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-Tc
ExpressionBacterialPromoterrbcS-Tc: T7, chaperones: T12Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2B2
Plasmid#160880PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S1: T7, chaperones: T7Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
18 pCOLI_A_Trx
Plasmid#160255PurposeN-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags6xHis and ThioredoxinExpressionBacterialPromoterT7Available SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
11 pCOLI_A_MBP
Plasmid#160247PurposeN-term MBP 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags6xHis and MBPExpressionBacterialPromoterT7Available SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13-IDR-mCherry
Plasmid#145280PurposeBacterial Expression of 6xHis HOXD13 IDR mCherry Fusion ProteinDepositorInsertHOXD13-IDR (HOXD13 Human)
ExpressionBacterialAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21c-His-Xa-Gluc-Xa-C9D-TGinsert
Plasmid#124660Purposereporter protein which emits bright blue fluorescenceDepositorInsertGaussia luciferase-C9D-TGinsert
TagsIEGRTMGDDDGDDDGDDD(FactorXa,TMG,C9D-Tag) and MHHH…ExpressionBacterialMutationchanged glutamic acid 100 to alanine, and glycine…PromoterT7 promoterAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT-9218
Plasmid#124225PurposeBacterial SpCas9 expressionDepositorInsertSpCas9
UseCRISPRExpressionBacterialPromoterTetR/TetAAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RNAPII-CTD wt #14-HA
Plasmid#160688PurposeExpression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.DepositorInsertRNA Polymerase II CTD
ExpressionBacterialMutationC terminal domain onlyAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLNtC60αC20C60β
Plasmid#160874PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
ExpressionBacterialPromoterrbcL: pBAD, chaperones: T7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLAtC60αβC20
Plasmid#160873PurposeExpression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
ExpressionBacterialPromoterrbcL: pBAD, chaperones: T7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
5 pCOLI_A_STREP
Plasmid#160147PurposeN-term 2xStrepII 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags2xStrepIIExpressionBacterialPromoterT7Available SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAD1
Plasmid#154347PurposepUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistanceDepositorInsertShBle
UseMicroalgaeExpressionBacterialMutationnonePromoterViolaxanthin Chlorophyll a Binding Protein Precur…Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP4
Plasmid#160087PurposeBacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4DepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EIF4E_IF4E
Plasmid#110010PurposeProtein expression and purification of EIF4E_IF4EDepositorInsertEIF4E_IF4E (EIF4E Human)
ExpressionBacterialAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR2F2_Hormone-recep
Plasmid#109826PurposeProtein expression and purification of NR2F2_Hormone-recepDepositorInsertNR2F2_Hormone-recep (NR2F2 Human)
ExpressionBacterialAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PLCG1_SH3
Plasmid#110035PurposeProtein expression and purification of PLCG1_SH3DepositorInsertPLCG1_SH3 (PLCG1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PTPN22_Y-phosphatase
Plasmid#110048PurposeProtein expression and purification of PTPN22_Y-phosphataseDepositorInsertPTPN22_Y-phosphatase (PTPN22 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_KPNB1_HEAT
Plasmid#110017PurposeProtein expression and purification of KPNB1_HEATDepositorInsertKPNB1_HEAT (KPNB1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only