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pHH04_32_sRNA-A_Sp_coD

Plasmid

#120900

Purpose

the component plasmid of a regulated device

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Inserts

pECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
sRNA-A

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-156

Plasmid

#118538

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD*RFPEC2

Plasmid

#17823

Depositor

Jay Keasling

Article

Pfleger et al Biotechnol Bioeng. 2005 Dec 5. 92(5):553

Insert

E. coli optimized red flourescent protein (RFPec2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA4-lenti

Plasmid

#115663

Purpose

Expresses AcrVA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARF6-V5-His6

Plasmid

#67415

Purpose

Expression of hArf6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBTK1003 (pSL2)

Plasmid

#190998

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAM002

Plasmid

#170006

Purpose

Encodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PpmrD

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-SMR1

Plasmid

#136361

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-SMR2

Plasmid

#136362

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR1

Plasmid

#136359

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR2

Plasmid

#136360

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcrRNA.con

Plasmid

#61285

Purpose

Constitutive repeat-only E. coli CRISPR array

Depositor

Chase Beisel

Article

Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX CrkL-SH2

Plasmid

#46419

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Crkl (CRKL Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 9-108

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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RAB2B

Plasmid

#25534

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

RAB2B (RAB2B Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBetI-E1

Plasmid

#74677

Purpose

BetI-E1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSE1-BetI-PBetI-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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p404CYC1

Plasmid

#15971

Depositor

Nicolas Buchler, Fred Cross

Insert

CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

CYC1 promoter is between SacI and XbaI restrictio…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mStrawberry-15b

Plasmid

#206868

Purpose

Express tTALE-mStrawberry

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mStrawberry

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-566

Plasmid

#104195

Purpose

Expresses human AR-1-566 from bacterial expression vector pGEX-5X-1 as GST fusion

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-566 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-10XHis-GST-HRV-dHer1cys

Plasmid

#73218

Purpose

Bacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)

Depositor

Marcel Bruchez

Article

Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

Insert

10XHis-GST-HRV-dHer1cys

Tags

10XHis-GST-HRV and The dimer of the Her1 Affibody…

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSY14-ptb

Plasmid

#90490

Purpose

pSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

sceC

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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