30,808 results

1. mCas13 Twin-SUMO

   Plasmid

   #199753

   Purpose

   Expresses codon optimized miniature mCas13 for protein expression in E.coli

   Depositor

   Magdy Mahfouz

   Article

   Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

   Insert

   mCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Promoter

   LacI

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PsaA

   Plasmid

   #159997

   Purpose

   Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   PsaA (19-309)
   

   Tags

   His6-tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. gZiPro

   Plasmid

   #198441

   Purpose

   Activity/inhibition assays, 19F-NMR

   Depositor

   Ute Hellmich, Tanja Schirmeister

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   glycine linked Zika virus NS2B-NS3
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   R95*A,

   Promoter

   T7 promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-EPN-09

   Plasmid

   #79924

   Purpose

   Palm3-12GS-I3-01-myc-Late1

   Depositor

   Neil King

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN-09
   

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pTriEx-mCherry-LINXa3-nano

   Plasmid

   #73616

   Purpose

   Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa3-nano
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSP72-deltaFlincG

   Plasmid

   #19733

   Depositor

   Wolfgang Dostmann

   Article

   Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   delta-FlincG (PRKG1 Bovine)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 77-356

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

7. PEP119T

   Plasmid

   #98251

   Purpose

   Pseudomonas T3SS gateway vector with HA-sfGFP11-sfGFP11

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   Gateway cassettee-HA-sfGFP11-11
   

   Expression

   Bacterial

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCWU6-xylR-E-lepB

   Plasmid

   #190725

   Purpose

   fine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible system

   Depositor

   Chenggang Wu

   Article

   Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

   Insert

   xylR-E-lepB
   

   Expression

   Bacterial

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYTK014

   Plasmid

   #65121

   Purpose

   Encodes pTEF2 as a Type 2 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   pTEF2
   

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a_Pro-IL-36β

    Plasmid

    #214344

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36B (IL36B Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a_Pro-IL-36α

    Plasmid

    #214343

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36A (IL36A Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103_PPP1CA_Metallophos

    Plasmid

    #109934

    Purpose

    Protein expression and purification of PPP1CA_Metallophos

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PPP1CA_Metallophos
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFP.R401

    Plasmid

    #138222

    Purpose

    Expresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(IGC).TerThyX*.(SFD)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFP.R360

    Plasmid

    #138230

    Purpose

    Expresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(HGK).Ama-Ter*.(SEL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJ211_WT_hi

    Plasmid

    #128850

    Purpose

    High copy number plasmid for expression of E. coli flagellin from its natural promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBM3R1-B2

    Plasmid

    #74679

    Purpose

    BM3R1-B2 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSB2-BM3R1-PBM3R1-YFP
    

    Expression

    Bacterial

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119067

    Purpose

    Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
    

    Expression

    Bacterial

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKEW106-MbCas12a-58069

    Plasmid

    #115653

    Purpose

    Expresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR control

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    MbCas12a
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST-PRDM16 1-223

    Plasmid

    #53346

    Purpose

    bacterial expression of mouse PRDM16 fragment

    Depositor

    Shingo Kajimura, Bruce Spiegelman

    Article

    Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1-223

    Promoter

    tac

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only