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pBXCA3GH

Plasmid

#47071

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), Avi-tag, GFP…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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HGS

Plasmid

#39101

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

HGS (HGS Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pET23b-proMMP-7

Plasmid

#29524

Depositor

William Parks

Article

Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-3

Insert

proMMP-7 (MMP7 Human)

Expression

Bacterial

Available Since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
p_SNIPR_target_mut_36

Plasmid

#139466

Purpose

T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

Target_mut36

Expression

Bacterial

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQA-LP574

Plasmid

#222953

Purpose

Expresses R120A LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 120 to Alanine

Promoter

T7

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MARK3_HUMAN_D0

Plasmid

#79740

Purpose

This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MARK3 (MARK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
ZP10 Snail

Plasmid

#16931

Depositor

Randall Moon

Insert

snail (snai1a Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET-NtLAtC60αβC20

Plasmid

#160873

Purpose

Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: pBAD, chaperones: T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJKW0776

Plasmid

#119024

Purpose

E.coli expression plasmid for PmSPS2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmSPS2

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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PTGDSA

Plasmid

#42452

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PTGDSA (PTGDS Human)

Tags

His6

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pJUMP18-merR_O

Plasmid

#127023

Purpose

Basic Part O- ORF; merR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part merR_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX cycT2b (P#1730)

Plasmid

#14603

Depositor

Matija Peterlin

Article

Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Insert

cycT2 (CCNT2 Human)

Tags

GST

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCas_5+7_gfp

Plasmid

#60595

Purpose

encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_5+7_gfp

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCas_2+5

Plasmid

#60594

Purpose

encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_2+5

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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GST-N1IC

Plasmid

#47612

Purpose

bacterial expression of GST-myc-Notch1 IC

Depositor

Urban Lendahl

Article

Beatus et al Development. 1999 Sep;126(17):3925-35.

Insert

Notch1 intracellular domain (Notch1 Mouse)

Tags

GST and myc

Expression

Bacterial

Mutation

contains amino acids 1753-2531

Promoter

tac

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
AND-gate-V2.0

Plasmid

#44449

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

AND-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pET-E(4130)A2

Plasmid

#168021

Purpose

Expresses E(4130)A2-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

E(4130)A2-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGST-PPARdelta

Plasmid

#16548

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR delta (PPARD Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal DNA-binding domain: aa 1-249

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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DLG4_PDZ_3

Plasmid

#103953

Purpose

Protein expression and purification of PSD95 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG4 (DLG4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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