30,807 results

1. HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

   Plasmid

   #99343

   Purpose

   Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

   Depositor

   Peter Wright

   Article

   Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

   Inserts

   Hif1a (776-826) (HIF1A Human)
   CBP (340-439)
   

   Tags

   His6GB1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_Nsp8

   Plasmid

   #156477

   Purpose

   Bacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSC-SmBiT

   Plasmid

   #207632

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-SmBiT
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCBB-ntH6-eGFP

   Plasmid

   #32557

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   6 His tag with Thrombin site

   Expression

   Bacterial

   Promoter

   Consitutive lac

   Available Since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET19b_4CL + STS

   Plasmid

   #139790

   Purpose

   expression of biosynthetic enzymes 4CL and STS

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Inserts

   4CL
   STS
   

   Expression

   Bacterial

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBR6k-con-GFPuv

   Plasmid

   #106387

   Purpose

   Constitutive LlacO-1 promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Expression

   Bacterial

   Promoter

   LlacO-1

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PaBphP 1-497

   Plasmid

   #36920

   Depositor

   Keith Moffat

   Article

   Yang et al Nature. 2011 Oct 16;479(7373):428-32. do

   Insert

   PaBphP-PCD
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains the photosensory core domain coding regi…

   Promoter

   T7

   Available Since

   May 18, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pFCyR5

   Plasmid

   #149480

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymR(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PCymRC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. SNX5-PX (26-170)

   Plasmid

   #119086

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX5-PX (26-170)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX5-PX (26-170) (SNX5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTJV1Sc2-tetA

    Plasmid

    #166982

    Purpose

    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    CspRecT
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pVanCC

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPro18-km

    Plasmid

    #17808

    Depositor

    Jay Keasling

    Article

    Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEXNKI-his-GST-3C-LIC

    Plasmid

    #108709

    Purpose

    Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    GST-6xHis-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEM7066

    Plasmid

    #214004

    Purpose

    PrpsM driven expression of ssrA-tagged mScarlet-I (LVA)

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    mScarlet-I-LVA
    

    Expression

    Bacterial

    Promoter

    PrpsM

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28 sf:mid.11

    Plasmid

    #191921

    Purpose

    Encodes a GFP design, sf:mid.11

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.11
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69M Y145F T167I L220V V224I

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28 sf:mid.7

    Plasmid

    #191933

    Purpose

    Encodes a GFP design, sf:mid.7

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.7
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145F V224I

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28 acid.6

    Plasmid

    #191912

    Purpose

    Encodes a GFP design, acid.6

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.6
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69L S72A Y145M T167V H181L

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28 sf:acid.3

    Plasmid

    #191926

    Purpose

    Encodes a GFP design, sf:acid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:acid.3
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69L S72A T108V Y145M V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28 mid.7

    Plasmid

    #191900

    Purpose

    Encodes a GFP design, mid.7

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.7
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145F V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b-mLin28A-cHIS

    Plasmid

    #137998

    Purpose

    Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

    Depositor

    Judy Lieberman

    Article

    O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

    Insert

    mouse Lin28A, CSD and ZnFs (residues 36–186)
    

    Tags

    C-terminal His6 tag

    Expression

    Bacterial

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH6HTC_NASP-Halo

    Plasmid

    #175340

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    NASP (NASP Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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