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pMAL-PUM2

Plasmid

#120385

Purpose

Bacterial expression of MBP-Pumillio2 fusion protein

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

PUM2 (PUM2 Human)

Tags

6xHIS and MBP

Expression

Bacterial

Promoter

LacI

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG216

Plasmid

#114002

Purpose

Level 2 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFAB815

Plasmid

#47854

Purpose

BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rnpB T1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO9

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pAH18

Plasmid

#154397

Purpose

Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome

Use

Synthetic Biology

Expression

Bacterial

Promoter

NA

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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HOXA10 (human) HIS-tag pET

Plasmid

#8516

Depositor

Corey Largman

Article

Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

Insert

HOXA10 (HOXA10 Human)

Expression

Bacterial

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
LB335 (Bcl-2 from ATG to -1281)

Plasmid

#15382

Depositor

Linda Boxer

Article

Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from ATG to -1281 with P2

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pAB061i2

Plasmid

#79229

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87A); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV Flag dPDZ-PTEN (1-400)

Plasmid

#22232

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

Insert

PTEN (Pten Mouse)

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

PDZ binding domain deleted. Contains amino acids …

Available Since

March 19, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET16b-Antp

Plasmid

#86866

Purpose

Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

Depositor

Michael Chin

Article

Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

Insert

Linker-Antp

Tags

Antp

Expression

Bacterial

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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Reb_65 aka PAS626

Plasmid

#71231

Purpose

Reb_1 with RebA(Y82A)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, Y82A)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pET22b-T5-mad32TAG

Plasmid

#188997

Purpose

Express madanin-1 with TAG at 32 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

madanin-1 with TAG at 32 position

Expression

Bacterial

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-mad

Plasmid

#188998

Purpose

Express wildtype madanin-1

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

wildtype madanin-1

Expression

Bacterial

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEDF-PhdRS

Plasmid

#127445

Purpose

Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

Depositor

Wenshe Liu

Article

Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

N346A-C348A mutations on PylRS

Promoter

araBAD

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMflET-o4

Plasmid

#101314

Purpose

Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Inserts

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
ereB resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRO16Sh2

Plasmid

#180604

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLac

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSG81-pLas-tetO-deGFPssrA

Plasmid

#102454

Purpose

Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Tags

ssrA

Expression

Bacterial

Promoter

pLas-tetO

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSS2 (hS100B)

Plasmid

#26774

Depositor

Gary Shaw

Article

Smith et al Biochemistry. 1996 Jul 9. 35(27):8805-14

Insert

S100B (S100B Human)

Expression

Bacterial

Available Since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pJJH1260

Plasmid

#36913

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Inserts

ScURA3
ScURA3

Expression

Bacterial, Mammalian, and Y…

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pH6HTC_BTF3-Halo

Plasmid

#175324

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

BTF3 (BTF3 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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