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pHAT

Plasmid

#112582

Purpose

Used for expression of protein in E. coli as non-cleavable N-terminal 6His-tag fusions

Depositor

Marko Hyvönen, Johan Peränen

Publication

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His

Expression

Bacterial

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28 His10-FcRɣ

Plasmid

#113021

Purpose

for purification of His10-FcRɣ intracellular domain

Depositor

Ron Vale

Publication

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

His10-FcRɣ intracellular domain

Expression

Bacterial

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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Pediculus humanus corporis PINK1 (143 - 575)

Plasmid

#110751

Purpose

Bacterial expression of PhPINK1 (crystallography)

Depositor

David Komander

Publication

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Tags

N-His6-SUMO

Expression

Bacterial

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAB078d9

Plasmid

#79207

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCD296.7

Plasmid

#113320

Purpose

dCas9, MCP-TetD, J107 scRNA.b1

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-TetD, J107 scRNA.b1_MS2

Use

CRISPR

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-8064.1

Plasmid

#111407

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 8064 bases in length (v1)

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-ACK

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-Pspac

Plasmid

#112778

Purpose

Promoter repressed by LacI (not natively produced in B. subtilis 168)

Depositor

Chris French

Publication

Wicke et al bioRxiv 185108

Insert

Pspac promoter

Expression

Bacterial

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCD175

Plasmid

#113316

Purpose

dCas9, MCP-AsiA, rpoD_F563Y

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-T4.AsiA, rpoD_F563Y

Use

CRISPR

Expression

Bacterial

Mutation

F563Y

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCB32

Plasmid

#111911

Purpose

Expresses gRNA to target Nte1 locus and has HygR marker

Depositor

Yi Tang

Insert

gRNA cassette targeting Nte1 locus

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

SNR52p

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK619

Plasmid

#110619

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pHL 636

Plasmid

#53015

Purpose

AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Inserts

fimE
fimB

Use

Synthetic Biology

Tags

Asp terminator, RBS(st7), and T1 terminator

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Endophilin SH3 domains

Plasmid

#47401

Depositor

Peter McPherson

Publication

Micheva et al J Biol Chem. 1997 Oct 24;272(43):27239-4

Insert

Endophilin

Tags

GST

Expression

Bacterial

Mutation

SH3 domain (aa 292-352)

Promoter

tac

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
Endophilin III Full

Plasmid

#47410

Depositor

Peter McPherson

Insert

Endophilin III

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pCRISPR_RAF1_94

Plasmid

#111826

Purpose

Knockout Raf1

Depositor

Steve Shih

Publication

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA targeting RAF1 94-114 (RAF1 Human)

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCDFNKI-his-3C-LIC-strep

Plasmid

#108705

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Publication

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

His-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-kan

Plasmid

#108706

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Publication

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET-2HMG-eGFP

Plasmid

#112737

Purpose

Express 2HMG-eGFP in E. coli

Depositor

Kyubong Jo

Publication

Lee et al RSC Adv, 2016, 6, 46291-46298

Insert

2HMG-eGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET his-mRFP

Plasmid

#113553

Purpose

For bacterial expression and purification of mRFP

Depositor

Jeanne Stachowiak

Publication

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

his-mRFP

Expression

Bacterial

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pME mKate2-dynactin1-811

Plasmid

#105971

Purpose

entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

Depositor

Caren Norden

Publication

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSe_31

Plasmid

#109235

Purpose

PUPsp1 GFP reporter

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PUPsp1

Available since

July 23, 2018

Availability

Academic Institutions and Nonprofits only

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