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GST-rhodopsin-CTS

Plasmid

#86071

Purpose

A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Rhodopsin (RHO Bovine)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 309-348 of Rhodopsin

Promoter

tac

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pQE30-rSyntenin1

Plasmid

#89428

Purpose

bacterial expression of rat Syntenin1

Depositor

Fritz Rathjen

Insert

SDCBP

Tags

His

Expression

Bacterial

Promoter

T5

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF-casBCDE(g8)

Plasmid

#89727

Purpose

Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

Depositor

Konstantin Kuznedelov, Konstantin Severinov

Publication

Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

CRISPR array

Expression

Bacterial

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCK300

Plasmid

#87766

Purpose

empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI

Depositor

John Heap

Publication

Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTS2

Plasmid

#87798

Purpose

For constructing SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

Depositor

Yu-Zhu Zhang

Publication

Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

Type

Empty backbone

Tags

6xHis and Strep II

Expression

Bacterial

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHT-497

Plasmid

#87756

Purpose

Bacterial expression vector for producing 6x histidine tagged rat DYRK1A kinase domain (residues 1-497)

Depositor

Yu-Wen Hwang

Insert

Gene coding for rat DYRK1A kinase domain (residues 1-497)

Tags

6X histidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L-MM

Plasmid

#89611

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

Depositor

Leemor Joshua-Tor

Publication

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L VVEL293MM Mutant (DOT1L Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET tdTomato LIC cloning vector (u-tdTomato)

Plasmid

#86926

Purpose

pET LIC cloning vector for yORF-tdTomato

Depositor

Chris Jeans

Type

Empty backbone

Tags

tdTomato

Expression

Bacterial

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSHS248

Plasmid

#87354

Purpose

S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

Depositor

Jennifer Doudna

Publication

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S/S867C/N1054C

Promoter

T7

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pTYB11-PTBP1-RRM1

Plasmid

#89153

Purpose

Recombinant expression of PTBP1-RRM1 in E.coli

Depositor

Frédéric Allain

Publication

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)

Tags

Intein and chitin binding domain

Expression

Bacterial

Promoter

T7

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-PTBP1-RRM34

Plasmid

#89156

Purpose

Recombinant expression of PTBP1-RRM34 in E.coli

Depositor

Frédéric Allain

Publication

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)

Tags

hexa His

Expression

Bacterial

Mutation

contains RNA Recognition Motif 3+4

Promoter

T7

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-PTBP1CCtoSS

Plasmid

#89157

Purpose

Recombinant expression of PTBP1 C250S,C251S in E.coli

Depositor

Frédéric Allain

Publication

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)

Plasmid

#87735

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Publication

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCRRNAcos

Plasmid

#78492

Purpose

Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.

Depositor

David Bikard

Publication

Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

Insert

tracrRNA

Expression

Bacterial

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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His 3C MKK3 1-347

Plasmid

#87878

Purpose

E. coli expression of MKK3 aa 1-347

Depositor

Peter Mace

Insert

MKK3 (MAP2K3 Human)

Tags

3C protease site and 6xHis

Expression

Bacterial

Mutation

aa 1-347

Promoter

T7

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET mRuby2 LIC cloning vector (u-mRuby2)

Plasmid

#86932

Purpose

pET LIC cloning vector for yORF-mRuby2

Depositor

Chris Jeans

Type

Empty backbone

Tags

mRuby2

Expression

Bacterial

Promoter

T7

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET mClover LIC cloning vector (u-mClover)

Plasmid

#86929

Purpose

pET LIC cloning vector for yORF-mClover

Depositor

Chris Jeans

Type

Empty backbone

Tags

mClover

Expression

Bacterial

Promoter

T7

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FbGH30

Plasmid

#86463

Purpose

Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

Depositor

Jan-Hendrik Hehemann

Publication

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FbGH30

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDnaK-Wasabi

Plasmid

#86110

Purpose

mWasabi reporter gene driven by DnaK promoter

Depositor

Mikhail Shapiro

Publication

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Tags

6xHis

Expression

Bacterial

Promoter

pDnaK

Available since

Feb. 25, 2017

Availability

Academic Institutions and Nonprofits only

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