31,184 results
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Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5052
Plasmid#128729PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAM1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGJJ001
Plasmid#183619PurposebindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA
Plasmid#108549PurposeBacterial Target-AID vectorDepositorInsertdCas9-PmCDA1
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mNeonGreen
Plasmid#205020PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorA-mNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL3
Plasmid#101912PurposeExpresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyExpressionBacterialPromoterT7+LacOAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AcrIIA2
Plasmid#101042PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA2
TagsGSTExpressionBacterialAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
DT037A
Plasmid#159398PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoterDepositorInsertpr.J23101-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialPromoterJ23101 (strong) promoterAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT31
Plasmid#89666Purposeexpression of doubleTAL staple protein dT31 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT31
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT27
Plasmid#89665Purposeexpression of doubleTAL staple protein dT27 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT27
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only