32,069 results

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pCoofy12

Plasmid

#43977

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

OneStrep

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pHCKan-INSyibp-Nef

Plasmid

#134593

Purpose

Low Phosphate inducible Nef

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

HIV Nef protein

Tags

His tag

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR:G249A

Plasmid

#79163

Purpose

Expresses inactive WspR G249A under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Mutation

G249A

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pKM061

Plasmid

#134174

Purpose

Bacterial expression of GST-HJURP

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

HJURP (HJURP Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pQMD

Plasmid

#48103

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

myc tag

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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REM2

Plasmid

#55682

Purpose

expression of mouse REM2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

REM2 (Rem2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
Dapp1-PH pET11a

Plasmid

#71376

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Dapp1-PH (DAPP1 Human)

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 148-273

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pED14xS-1

Plasmid

#134800

Purpose

IPTG inducible expression of S-TAGA qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

S-TAGA qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED14xS-WT

Plasmid

#134804

Purpose

IPTG inducible expression of WT S tRNA scaffold tRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Bottom-T

Plasmid

#87310

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-SpyTag003

Plasmid

#216282

Purpose

Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SUMO-SpyTag003

Tags

His6 and SpyTag003

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBS/KS-mId3sp

Plasmid

#16045

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id3 (Id3 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 365-635

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pRG/III-hs-MBP-T43NTR-TrxA-H10

Plasmid

#121677

Purpose

Bacterial expression of neurotensin receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60

Insert

neurotensin receptor (Ntsr1 Rat)

Tags

TrxA-H10

Expression

Bacterial

Mutation

deleted amino acids 1-42

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
pBEAST_J23101-bphC

Plasmid

#128137

Purpose

The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

bphC

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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cp193g7

Plasmid

#25950

Depositor

Robert Campbell

Article

Carlson et al Protein Sci. 2010 Aug;19(8):1490-9. doi:

Insert

cp193g7

Tags

His

Expression

Bacterial

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
pGEX4T1-GST-eIF4G1-His6

Plasmid

#37231

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF4631 (TIF4631 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

tac

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pKS13

Plasmid

#24627

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

pdc, adhB

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
pET28a(+) 6xHis-TEV-IFIT2

Plasmid

#53558

Purpose

bacterial expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pET15b I-SceI (Ctermhis -MH)

Plasmid

#195613

Purpose

Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

Depositor

Frederick Gimble

Article

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_VCP_AAA

Plasmid

#109964

Purpose

Protein expression and purification of VCP_AAA

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VCP_AAA (VCP Human)

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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