30,410 results

1. pGEX4T-hLIG3

   Plasmid

   #81055

   Purpose

   bacterial expression of human LIG3

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   DNA ligase 3 (LIG3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-mTurquoise2-CNA35

   Plasmid

   #61602

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mTurqouise and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAWP78-PVCpnf1-16

   Plasmid

   #198271

   Purpose

   Unmodified pPVC; contains WT PVC structural/accessory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf1-16
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-HaloTag-KanMX6

   Plasmid

   #87029

   Purpose

   Tag S. pombe genes in C terminal with HaloTag

   Depositor

   Julien Berro

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC101-AmpR EV

   Plasmid

   #208184

   Purpose

   empty plasmid vector, non-fluorescent

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Type

   Empty backbone

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLp_3050sNuc

   Plasmid

   #122030

   Purpose

   Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

   Depositor

   Geir Mathiesen, Vincent Eijsink

   Article

   Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

   Insert

   signal peptide Lp_3050
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sppA

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C3-hYAP1

   Plasmid

   #17843

   Depositor

   Marius Sudol

   Article

   Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL99

   Plasmid

   #78577

   Purpose

   Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system

   Depositor

   Xuming Mao

   Article

   Sun et al Annals of Microbiology Dec 2012, Volume

   Type

   Empty backbone

   Use

   Streptomyces expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   inducible PnitA-NitR system

   Available Since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIV643

    Plasmid

    #226182

    Purpose

    Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    uidA marker flanked with targeting regions to KU70 gene in K.phaffii
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TEF1p

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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