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GST-Hsp90 M(272-617)

Plasmid

#22482

Purpose

Please note this plasmid contains GST-Hsp90 residues 273-617.

Depositor

William Sessa

Publication

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha M domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGEX-2TK cyclin T1 (P#468)

Plasmid

#14607

Depositor

Matija Peterlin

Publication

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (CCNT1 Human)

Tags

GST

Expression

Bacterial

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAKlux2.1

Plasmid

#14081

Depositor

Attila Karsi

Publication

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Expression

Bacterial

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212829

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

Depositor

Wolfgang Enard

Publication

Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Promoter

CAG, TetOn

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4)P Probe

Plasmid

#211509

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SidC (P4C)

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pB025

Plasmid

#183092

Purpose

Expresses FnCas12a in Bacteroides and used for genome editing

Depositor

Lei Dai

Publication

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Insert

FnCas12a, gRNA, Promoter, HAB, TetR,

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P1TDPGH023

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNIC28-MacroGreen

Plasmid

#160665

Purpose

Expresses GFP fusion of a multisite mutant of the Af1521 protein

Depositor

Herwig Schüler

Publication

Garcia-Saura et al Commun Biol. 2021 Jul 28;4(1):919. doi:

Insert

Af1521 modified synthetic construct eGFP fusion

Tags

eGFP and hexahistidine_TEV recognition

Expression

Bacterial

Promoter

LacI

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Publication

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Expression

Bacterial

Promoter

Arabinose-inducible pBAD

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαi1-LgB91

Plasmid

#134342

Purpose

Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

Depositor

Julien Hanson

Publication

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gαi1-LgB91 (GNAI1 Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCasKP-hph

Plasmid

#117232

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

Plasmid

#117006

Purpose

Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-SpyTag002-MBP

Plasmid

#102831

Purpose

Bacterial expression of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Publication

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to the N-terminus of MBP via a …

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEB1-mGFPmut2

Plasmid

#103980

Purpose

Plasmid encoding mGFPmut2

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65A, V68L, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEThT-RvLEAM

Plasmid

#90033

Purpose

To express His-tagged RvLEAM in bacteria

Depositor

Takekazu Kunieda

Publication

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Tags

His

Expression

Bacterial

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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4xNLS-pMJ915v2-sfGFP

Plasmid

#88921

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUCP20T-tdTomato

Plasmid

#78469

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Publication

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

tdTomato

Expression

Bacterial

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9cur

Plasmid

#71538

Purpose

Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

Depositor

Tao Chen

Publication

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Inserts

Cas9
Plasmid curing system

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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V48 ELP

Plasmid

#68395

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

Depositor

Andrew MacKay

Publication

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V48 ELP

Expression

Bacterial

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pETM6-E12-vioABECD

Plasmid

#66537

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

Depositor

Mattheos Koffas

Publication

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOPINS3C

Plasmid

#41115

Depositor

Ray Owens

Publication

Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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