30,736 results

1. 6xHis-SNAP-PI(3,4)P2 Probe

   Plasmid

   #211511

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   TAPP1 cPHx3
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPEgRNA

   Plasmid

   #172716

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   gRNA
   

   Expression

   Bacterial

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. miniTurbo-His6_pET21a

   Plasmid

   #107178

   Purpose

   expresses His6-tagged miniTurbo in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-T7-3WJdB-T

   Plasmid

   #87308

   Purpose

   Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Three-way junction dimeric Broccoli aptamer
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP1360

   Plasmid

   #119278

   Purpose

   for cloning new sgRNAs

   Depositor

   Jason Peters, Carol Gross, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. FABP1A

   Plasmid

   #42344

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   FABP1A (FABP1 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pGP-RSET-iGABASnFR2-6xHis

   Plasmid

   #218885

   Purpose

   Bacterial expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   T7

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExp-NHis

   Plasmid

   #112558

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-CjCas9

   Plasmid

   #89754

   Purpose

   Expression of CjCas9 with His tag in E.coli

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   6XHis, HA, and SV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CDC34

    Plasmid

    #25598

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CDC34 (CDC34 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pTCF236 HLA-B*40:01

    Plasmid

    #180463

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*40:01
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLG004 (RT-Abi-P2)

    Plasmid

    #157882

    Purpose

    Expresses the RT-Abi-P2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RT-Abi-P2
    

    Expression

    Bacterial

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJV53

    Plasmid

    #26904

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Che9c genes 60-61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pThermoCas9_ctrl

    Plasmid

    #100981

    Purpose

    Expresses ThermoCas9 and its sgRNA module

    Depositor

    John van der Oost

    Article

    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

    Inserts

    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    

    Tags

    None

    Expression

    Bacterial

    Promoter

    B. coagulans DSM 1 pta promoter and B. smithii xy…

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-mE1

    Plasmid

    #32534

    Depositor

    Jorge Eduardo Azevedo

    Article

    Carvalho et al Mol Biotechnol. 2011 Oct 20.

    Insert

    E1, ubiquitin-activating enzyme (Uba1 Mouse)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b RNase A

    Plasmid

    #58903

    Purpose

    Bacterial expression of bovine pancreatic ribonuclease A

    Depositor

    Ronald Raines

    Article

    delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

    Insert

    RNase A (RNASE1 Bovine)
    

    Tags

    pelB sequence

    Expression

    Bacterial

    Mutation

    See Depositor Comments below

    Promoter

    T7

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-hsFlt3L

    Plasmid

    #25074

    Depositor

    Maria Castro

    Article

    Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

    Insert

    human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)
    

    Expression

    Bacterial

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSR58.6

    Plasmid

    #63176

    Purpose

    Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaR
    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE30-His-NDK

    Plasmid

    #124136

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Nucleotide diphosphate kinase (ndk Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30_Halotag_K73T_v2

    Plasmid

    #183692

    Purpose

    Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(K73T)
    

    Expression

    Bacterial

    Mutation

    K73T in Halotag

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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