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31,561 results

Showing: 1141 - 1160 of 31561 results

  5. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Tags
    6xHis-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129593-plasmid-map-sequence-id-254271

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  6. pTara:500

    Plasmid
    #60717
    Purpose
    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60717-plasmid-map-sequence-id-97574

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  8. pBad-HPII TAG392

    Plasmid
    #105848
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 392 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 392
    Promoter
    pBad/arabinose
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105848-plasmid-map-sequence-id-205565

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  10. pHL 1454

    Plasmid
    #53019
    Purpose
    AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    fimB
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Promoter
    pLlac0-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pAH107

    Plasmid
    #248727
    Purpose
    PBT2364 biosensor circuit in pNBU2 integrative Bacteroides plasmid
    Depositor
    Carolina Tropini
    Insert
    PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT2364-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
    Expression
    Bacterial
    Available Since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    248727-plasmid-map-sequence-id-496769

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  12. pJB163

    Plasmid
    #248755
    Purpose
    PBT0779+10xTetO biosensor cicuit in pNBU2 integrative Bacteroides plasmid
    Depositor
    Carolina Tropini
    Insert
    PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-10xTetO-T7Te-rrnBT1-PBT0779-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248755-plasmid-map-sequence-id-496765

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  13. pSJX023

    Plasmid
    #232326
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Promoter
    PJ23119 and Pxyl
    Available Since
    Jan. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    232326-plasmid-map-sequence-id-496249

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  17. pSHDY*in_PpetE:BCD2:MrBBS:Strep

    Plasmid
    #133973
    Purpose
    Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    MrBBS:StrepII
    Use
    Synthetic Biology
    Tags
    StrepII
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available Since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133973-plasmid-map-sequence-id-267788

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  19. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46569-plasmid-map-sequence-id-364492

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  20. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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Showing: 1141 - 1160 of 31561 results