30,736 results

1. pGT-Kh2

   Plasmid

   #122587

   Purpose

   MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-nanoHER2-SpyTag003

   Plasmid

   #216312

   Purpose

   Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   nanoHER2-SpyTag003
   

   Tags

   His6 tag and SpyTag003

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a MDHM_YEAST

   Plasmid

   #204231

   Purpose

   Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHM_YEAST (MDH1 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Does not include transit peptide and includes a M…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Raf-1 GST RBD 1-149

   Plasmid

   #13338

   Depositor

   Channing Der

   Article

   Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

   Insert

   Raf-1 (RAF1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPC010.AAV

   Plasmid

   #171175

   Purpose

   Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   BBa_J23119 and Sp.pCas9, BBa_J23107

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCasAb-apr

   Plasmid

   #121998

   Purpose

   Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSW002-Pc-mRuby2

   Plasmid

   #111250

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mRuby2
   

   Expression

   Bacterial

   Mutation

   mRuby2 is codon optimized for expression in P. fl…

   Promoter

   Pc

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P2(GST-SNAP-Shp2tSH2)

   Plasmid

   #177907

   Purpose

   Baculo expression of GST tag fused to SNAPtag and human Shp2 tandem SH2 domains

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   Shp2 tandem SH2 domains (aa 6-218 only) (PTPN11 Human)
   

   Tags

   GST-PreScission cut site-SNAP tag

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLS416

   Plasmid

   #239127

   Purpose

   Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

   Type

   Empty backbone

   Use

   Centromeric

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. ReU_pET29b

    Plasmid

    #215426

    Purpose

    Expression construct for ReU gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    ReU
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEM7063

    Plasmid

    #214001

    Purpose

    PrpsM driven expression of ssrA-tagged eGFP (LVA)

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    eGFP-LVA
    

    Expression

    Bacterial

    Promoter

    PrpsM

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PCBD2A-c103

    Plasmid

    #53624

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    PCBD2A
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET MBP His6 LIC cloning vector (2Cc-T)

    Plasmid

    #37237

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM201

    Plasmid

    #13078

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    Ptac-gam-bet-exo operon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-RBD

    Plasmid

    #15247

    Depositor

    Martin Schwartz

    Article

    Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

    Insert

    Rhotekin (Rtkn Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 7-89 (Rho binding domain)

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. hsDCX-pHAT

    Plasmid

    #83928

    Purpose

    hsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

    Depositor

    Gary Brouhard

    Article

    Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

    Insert

    human Doublecortin isoform b (DCX Human)
    

    Tags

    6-His tag and Strep-TagII

    Expression

    Bacterial

    Available Since

    Dec. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. USP2

    Plasmid

    #36894

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP2 (USP2 Human)
    

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

    Plasmid

    #228663

    Purpose

    Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Zika virus portion of NS2B protease coexpressed with NS3 protease
    

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pET29b-AK1-His

    Plasmid

    #124135

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Adenylate kinase (AK1 Chicken)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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