30,736 results

1. PG1424PGA

   Plasmid

   #74653

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PG1424PGA
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pET47b-T7-enAsCas12f

   Plasmid

   #204642

   Purpose

   Expression of enAsCas12f in E. coli

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   enAsCas12f
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D196K, N199K, G276R, N328G, D364R

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-Streptavidin-Glutamate_Tag

   Plasmid

   #46367

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

   Insert

   Streptavidin-Glutamate_Tag
   

   Tags

   6 glutamate tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET-45b(+)-Tn5

   Plasmid

   #112112

   Purpose

   Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

   Depositor

   Frank Pugh

   Article

   Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

   Insert

   Tn5
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E54K E110K P242A L372P

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-tdTomato-CNA35

   Plasmid

   #61606

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   tdTomato and CNA35
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. RARS_pNIC-Bio3

   Plasmid

   #153055

   Purpose

   Expression construct for producing Avi tagged Arginyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   RARS (RARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pM2s2AsG

   Plasmid

   #137924

   Purpose

   E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   Superfolder GFP
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMA7CR_2.0

   Plasmid

   #73950

   Purpose

   pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Inserts

   cas9
   lambda beta
   dam
   recX
   

   Expression

   Bacterial

   Promoter

   pAra and pTet

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pATetO 6XHis T4 DNA Ligase

   Plasmid

   #206207

   Purpose

   Inducible expression of T4 DNA ligase

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   T4 DNA ligase
   

   Expression

   Bacterial

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19 Giles integrase

    Plasmid

    #170049

    Purpose

    pUC19 with Giles integrase

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Insert

    GIles Integrase
    

    Expression

    Bacterial

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T-1 3xFlag-JNK1a1

    Plasmid

    #47574

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    JNK1a1 (MAPK8 Human)
    

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pET30a-HS-Tn5

    Plasmid

    #127916

    Purpose

    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Insert

    Hyperstable Tn5
    

    Tags

    E coli elongation factor Ts and Mxe intein - Chit…

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCN34e-yfp

    Plasmid

    #227641

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCN34e-kimA-yfp

    Plasmid

    #227638

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    kimA-yfp
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a(+)-T7-OmpA-MNaseA-6xHis

    Plasmid

    #214808

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease A
    

    Tags

    6xhis

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBbA6c-RFP

    Plasmid

    #35290

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. Butelase AY

    Plasmid

    #221847

    Purpose

    Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

    Insert

    Butelase 1 ligase
    

    Expression

    Bacterial

    Mutation

    Val237Ala, Thr238Tyr

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZE21-GFPaav

    Plasmid

    #26643

    Depositor

    Michael Elowitz

    Article

    Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

    Insert

    pZE21-GFPaav
    

    Tags

    GFPaav

    Expression

    Bacterial

    Mutation

    na

    Available Since

    June 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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