30,258 results

1. 1B-GFP1-10

   Plasmid

   #193867

   Purpose

   E. coli expression vector for Hisx6 tagged GFP1-10.

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   sfGFP1-10
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETDuet S2

   Plasmid

   #171921

   Purpose

   Express RNA-based (p)ppGpp sensor and a NirFP

   Depositor

   Mingxu You

   Article

   Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

   Inserts

   RNA-based (p)ppGpp sensor
   NirFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. spNW100

   Plasmid

   #173488

   Purpose

   Expresses spNW100 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW100
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   P1049L- please see depositor comments

   Promoter

   T7

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pwtCas9-bacteria

   Plasmid

   #44250

   Purpose

   aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   wild-type Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YopH (residues 164-468)

   Plasmid

   #79749

   Purpose

   This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   YopH (residues 164-468)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Cys-60nmSAH-SPYcatcher

   Plasmid

   #214840

   Purpose

   This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   Cys-60nmSAH-SPYcatcher
   

   Use

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRelA*

   Plasmid

   #175590

   Purpose

   Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Catalytic domain of E.coli relA gene.
   

   Use

   Tags

   Fused with a flexible GS linker to mVenus

   Expression

   Bacterial

   Mutation

   Only the catalytic domain of the enzyme is used (…

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNCS BFP

   Plasmid

   #91757

   Purpose

   Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Blue Fluorescent Protein
   

   Use

   Tags

   Hexa-Histidine, Xpress epitope for detection with…

   Expression

   Bacterial

   Mutation

   GFP (Y66H)

   Promoter

   T7

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDM042

   Plasmid

   #216812

   Purpose

   Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mScarlet3
   

   Use

   Tags

   VinnyLinker

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3993 pET ubcH7

    Plasmid

    #8654

    Depositor

    Peter Howley

    Article

    Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

    Insert

    UbcH7 (UBE2L3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pTriEx-RhoA-wt_mScarlet-i_SGFP2

    Plasmid

    #85071

    Purpose

    Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
    

    Use

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pComb3HTT

    Plasmid

    #63900

    Purpose

    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.

    Depositor

    Carlos Barbas

    Article

    Barbas et al Methods, A companion to Methods in Enzym

    Insert

    TT Fab
    

    Use

    Phage display

    Tags

    geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD_mCherry_GFP_-+

    Plasmid

    #187390

    Purpose

    arabinose-inducible expression of GFP and mCherry

    Depositor

    Roi Avraham

    Insert

    GFP, mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTF-ahpC-rfp

    Plasmid

    #153035

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. plYJP022-int7

    Plasmid

    #202287

    Purpose

    Plasmid containing integrase for LPattB7 site

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    int7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX4T3-GST-Tev

    Plasmid

    #177142

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    Tev
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SPLICSFAST-LDh-LY-P2A

    Plasmid

    #214895

    Purpose

    Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-LDh-LY-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-24a-ELP[V-30]

    Plasmid

    #67014

    Purpose

    Expresses ELP[V-30] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    ELP[V-30]
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTlpA-Wasabi

    Plasmid

    #85455

    Purpose

    mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A

    Promoter

    TlpA and TlpA, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge