Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,295 results

Showing: 1281 - 1300 of 32295 results

  8. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

    Enlarge

  10. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Anat Herskovits
    Article
    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)
    Insert
    pheS
    Expression
    Bacterial
    Mutation
    A309G
    Available Since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98783-plasmid-map-sequence-id-189220

    Enlarge

  16. pEcTrpRSH6

    Plasmid
    #177598
    Purpose
    Expresses His tagged TrpRS in Escherichia coli cells
    Depositor
    Kambiz Hamadani
    Insert
    Tryptophan tRNA Synthetase (trpS Escherichia coli)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    177598-plasmid-map-sequence-id-351508

    Enlarge

  17. pEcH6ArgRS

    Plasmid
    #177582
    Purpose
    Expresses His tagged ArgRS in Escherichia coli cells
    Depositor
    Kambiz Hamadani
    Insert
    Arginine tRNA Synthetase (argS Escherichia coli)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    177582-plasmid-map-sequence-id-350694

    Enlarge

  18. pEcCysRSH6

    Plasmid
    #177585
    Purpose
    Expresses His tagged CysRS in Escherichia coli cells
    Depositor
    Kambiz Hamadani
    Insert
    Cysteine tRNA Synthetase (cysS Escherichia coli)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    177585-plasmid-map-sequence-id-356947

    Enlarge

  19. pET30a-SSDH

    Plasmid
    #239573
    Purpose
    Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    William Plaxton
    Article
    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b
    Insert
    Succinate-semialdehyde dehydrogenase (gabD E. coli)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239573-plasmid-map-sequence-id-476594

    Enlarge

Showing: 1281 - 1300 of 32295 results