30,736 results

1. pNatA (pACYCduet-naa10-naa15)

   Plasmid

   #72928

   Purpose

   Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   naa10 (naa10 Fission Yeast, Synthetic)
   naa15 (naa15 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Codon optomised for E.coli expression

   Promoter

   T7

   Available Since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET N6his GFP1-9

   Plasmid

   #182240

   Purpose

   Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

   Depositor

   Stéphanie Cabantous

   Article

   Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

   Insert

   GFP1-9
   

   Tags

   6HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. BirA His-tag pLEMO

   Plasmid

   #119817

   Purpose

   Express endogenous biotin ligase in E.coli

   Depositor

   Alexander Gabibov

   Article

   Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

   Insert

   Biotin ligase
   

   Tags

   6-Histidine-tag

   Expression

   Bacterial

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. spNW100

   Plasmid

   #173488

   Purpose

   Expresses spNW100 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW100
   

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   P1049L- please see depositor comments

   Promoter

   T7

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE 80L Kan T7WT

   Plasmid

   #102790

   Purpose

   Expresses T7 RNA polymerase

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   T7 RNA polymerase
   

   Expression

   Bacterial

   Available Since

   July 3, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pKL2299

   Plasmid

   #186332

   Purpose

   This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.

   Depositor

   Kan Wang

   Article

   Kang et al Front Plant Sci. 2022 May 4;13:860971. d

   Insert

   virulence genes from Agrobacterium tumefaciens pTiBo542
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPdR

   Plasmid

   #85083

   Purpose

   Expresses putidaredoxin reductase in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin reductase
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF-GFPplus

   Plasmid

   #172718

   Purpose

   Expression of a fast folding GFP variant GFP plus

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   GFP plus
   

   Expression

   Bacterial

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pF4Ag NanoLuc (ATG-42)

    Plasmid

    #137777

    Purpose

    Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

    Depositor

    Lance Encell

    Article

    Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

    Insert

    NanoLuc (Nluc)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NanoLuc contains 16 amino acid changes compared t…

    Promoter

    T7 and CMV

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid

    #119945

    Purpose

    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Renilla luciferase
    

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Promoter

    TetCMV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPdX

    Plasmid

    #85084

    Purpose

    Expresses putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Cysteine 73 to Serine and Cysteine 85 to …

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL2787

    Plasmid

    #15460

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    phage phi11 int gene
    

    Expression

    Bacterial

    Available Since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSJL1

    Plasmid

    #71261

    Purpose

    E. coli-Veillonella shuttle plasmid

    Depositor

    Felicia Qi

    Article

    Liu et al Appl Environ Microbiol. 2012 May;78(9):3

    Type

    Empty backbone

    Use

    E. coli-veillonella shuttle plasmid

    Expression

    Bacterial

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pET22b-T7-6h-GST-α-Synuclein

    Plasmid

    #225224

    Purpose

    Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

    Inserts

    GST
    α-Synuclein
    

    Tags

    6xHis Tag and TEV Cleavage Site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. MARS_pNIC-CTB10H

    Plasmid

    #153053

    Purpose

    Expression construct for producing Avi tagged Methionyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    MARS (MARS1 Human)
    

    Tags

    TEV-Avi-His10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbB8k-csg-NbGFP

    Plasmid

    #166858

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

    Insert

    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGRG36

    Plasmid

    #16666

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

    Insert

    mTn7::MCS
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDule2-pCNF

    Plasmid

    #85495

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC-SP6

    Plasmid

    #102418

    Purpose

    Ac transposase with SP6 promoter for in vitro transcription

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    Ac transposase
    

    Expression

    Bacterial

    Promoter

    SP6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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