33,902 results
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Plasmid#174076PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,DepositorInsertSuperfolder GFP
TagsHis-6ExpressionBacterialMutationN150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRPN265NB-LaG16-MNase
Plasmid#170978PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/LaG16/MNase
TagsGSTExpressionBacterialAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRecT_2
Plasmid#167546PurposeEncodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.DepositorInsertE. faecium Com15 recT under an IPTG inducible promoter, with lacI
ExpressionBacterialPromoterIPTGAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pdCas9-sgRNA-RFP
Plasmid#166005PurposeBacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mGreenLantern
Plasmid#161913PurposemGreenLantern fluorescent protein: bacterial expression plasmidDepositorInsertmGreenLantern
Tags6xHisExpressionBacterialPromoteraraBAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαq-LgB97 (GNAQ Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AckRS
Plasmid#137976Purposesequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansionDepositorInsertAckRS and pylTcua
ExpressionBacterialPromoteraraBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC-GFP-AT
Plasmid#133306Purpose"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisationDepositorInsertaxe/txe toxin-antitoxin cassette
UseSynthetic BiologyExpressionBacterialAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorExpressionBacterial and MammalianPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
TagsMet-6xHisExpressionBacterialPromoterT5 promoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAJM.336
Plasmid#108528PurposeLacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.DepositorInsertLacIAM + PTac-YFP
ExpressionBacterialMutationLacI Q18M A47V F161YAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
ExpressionBacterialAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC18-mini-Tn7T-Gm-lacZ
Plasmid#63120Purposemini-Tn7 lacZ transcriptional fusion vectorDepositorPublicationTypeEmpty backboneExpressionBacterialAvailable sinceMay 17, 2018AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDB016
Plasmid#99220PurposeAccessory plasmid for aaRS PACE; negative selectionDepositorInsertPT7 [SD8] gIII-neg
ExpressionBacterialPromoterPT7Available sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pProEx-IDE-wt
Plasmid#99014PurposeExpress human Insulin Degrading Enzyme (IDE) in E. coli.DepositorAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS YFP
Plasmid#91759PurposeBacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)DepositorInsertVenus YFP (A206K)
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZA16mflon
Plasmid#75439PurposePlasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.DepositorInsertmf-lon
ExpressionBacterialPromoterpBADAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1
Plasmid#78462PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; Q2513R (please see depositor commen…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMAL-c4X
Plasmid#75288PurposeExpresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable sinceJuly 19, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by