30,737 results
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Plasmid#199378PurposeEncodes dual cistronic cytosolic jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP7s::SL2::mScarlet-I
ExpressionBacterialMutationno mutationsAvailable SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-mNeongreen
Plasmid#182389PurposeBacterial expression of Vesicle Nucleating peptide15-mNeongreen fusionDepositorInsertVNp15-mNeongreen-His
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD103
Plasmid#119887PurposeConstitutive expression of mCherry on pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpConstitutive_mCherry
ExpressionBacterialAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp43(exo-)
Plasmid#228202PurposeExpression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219ADepositorInsert43
TagsSce VMA intein-chitin binding domainExpressionBacterialMutationMutated aspartate 219 to alanine (D219A), elimina…PromoterT7Available SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-GST
Plasmid#42049DepositorInsertGST
TagsHisExpressionBacterialAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrCAB
Plasmid#174618PurposeBacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.DepositorInsertmtrCAB
UseSynthetic BiologyExpressionBacterialPromoterpLacIAvailable SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP 150TAG
Plasmid#85483PurposeArabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
ExpressionBacterialMutation150TAG (amino acid 150 mutated to stop codon)PromoterArabinoseAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII
Plasmid#122082PurposeExpress M.EcoGII with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII
UseRetroviralTagsV5 tag on Nter of M.EcoGIIExpressionBacterial and MammalianAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIVM_26
Plasmid#204501PurposeExpression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)DepositorExpressionBacterialMutation0PromoterAmpRAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
OmpA3-PNGaseF-TEV-His6
Plasmid#114274PurposeAmidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.DepositorInsertPeptide: N-glycosidase F
TagsOmpA3 leader sequence (for periplasmic localisati…ExpressionBacterialPromoterT7Available SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)
Plasmid#55209DepositorTypeEmpty backboneTags10xAsn, 10xHis, MPB, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNR220
Plasmid#79541PurposeInput plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.DepositorInsertsTP901
BxbI
A118
TagsHis tag and LAA degradation tagExpressionBacterialAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mRuby3
Plasmid#74234PurposeConstitutive expression of mRuby3 in bacteriaDepositorInsertmRuby3
Tags6xHisExpressionBacterialPromoterT7Available SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LY-MT-P2A
Plasmid#214897PurposeExpresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sitesDepositorInsertSPLICSFAST-LY-MT-P2A
TagsnoneExpressionBacterial and MammalianAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBintE
Plasmid#48136PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHis-hTG2
Plasmid#100719PurposeExpression of human transglutaminase 2 in E.coliDepositorInsertTGM2 (TGM2 Human)
TagsHisExpressionBacterialMutationsilent mutation Arg580 (AGG--CGT) to enhance expr…PromoterT7Available SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISYmu1_reRNA
Plasmid#205358PurposeBacterial expression of ISYmu1 TnpB and its reRNADepositorInsertISYmu1 TnpB
ExpressionBacterialAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBIS
Plasmid#17817DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miniGFP2
Plasmid#188897PurposeExpress 6xHis-Tag miniGFP2 in E.coliDepositorInsertminiGFP2
Tags6xHisExpressionBacterialPromoteraraBADAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only